Process for preparing transformed microorganism, D-aminoacylase

Abstract

A transformed microorganism prepared by inserting into a host microorganism with zinc tolerance a D-aminoacylase-producing gene which selectively produces D-aminoacylase alone between D-aminoacylase and L-aminoacylase. A process comprising culturing the transformed microorganism in a culture medium containing zinc ion and obtaining D-aminoacylase from the culture at a high efficiency.

Description

technical field [0001] The present invention relates to the introduction of a D-acylated amino acid hydrolase gene that selectively generates D-acylated amino acid hydrolase among D-acylated amino acid hydrolase and L-acylated amino acid hydrolase into a zinc-tolerant microorganism The transformed microorganism formed in the invention, and the preparation method of D-acylated amino acid hydrolase using the transformed microorganism. Background technique [0002] D-amino acids with high optical purity are amino acids required for the production of side chains of antibiotics and polypeptide drugs, and D-acylated amino acid hydrolase is an enzyme used in industry to prepare D-amino acids. [0003] At present, as microorganisms capable of simultaneously producing D-acylated amino acid hydrolase and L-acylated amino acid hydrolase, Pseudomonas ( Pseudomonas sp.) AAA6029 strain; Actinomyces such as Streptomyces olivaceus S.62 reported in Japanese Patent Laid-Open No. Sho 53-59092...

AI Technical Summary

Problems solved by technology

When using this strain, although the trouble of separating D-acylated amino acid hydrolase and L-acyl amino acid hydrolase is saved, the production ability of D-acylated amino acid hydrolase is insufficient.

In addition, since the nucleotide sequence of the D-acyl amino acid hydrolase production gene is not clarified in JP-A-1-5488, it is impossible to modify the gene to improve the production ability of D-acyl amino acid hydrolase and to create a high-productivity transformed microorganisms

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