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Large yellow croaker mitochondrial molecule mark and its use in identification for cultivated population and wild population

A molecular marker, technology for large yellow croaker, applied in the direction of microbial determination/inspection, biochemical equipment and methods, etc., can solve problems such as can not be used as a reliable standard to distinguish between farmed and wild large yellow croaker, and price fraud.

Inactive Publication Date: 2005-03-02
XIAMEN UNIV
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  • Summary
  • Abstract
  • Description
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AI Technical Summary

Problems solved by technology

Because experience often makes mistakes and prices are cheating, these two methods cannot be used as reliable standards for distinguishing farmed and wild large yellow croakers. Especially in the case of disputes, a stable and reliable identification method is needed

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052]Example 1: Select the wild large yellow croaker of Naozhou nationality and the large yellow croaker cultured in the market, design and synthesize a pair of conservative primers L14841: 5'-CCATCCAACATCTCAGCATGATGAAA-3'; H15149: 5'-GCCCCTCAGAATGATATTTGT CCTCA-3'; Pigment B (cyt b) gene was amplified by PCR. The amplification program was 95°C 5min→(94°C 60sec→55°C 60sec→72°C 80sec)×35 cycles→72°C 5min→4°C∞; the amplification result was purified , Sequencing, and comparing the sequencing results.

Embodiment 2

[0053] Embodiment 2: Similar to Embodiment 1, the difference is that the wild large yellow croaker is selected from the wild large yellow croaker of the Fujian-Guangdong ethnic group.

Embodiment 3

[0054] Embodiment 3: Similar to Embodiment 1, the difference is that the wild large yellow croaker is selected from the large wild yellow croaker of the Daiqu nationality.

[0055] The sequencing results of Examples 1-3 show that the amplification results of the three wild populations of large yellow croaker are consistent, stable and highly reproducible.

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PUM

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Abstract

The present invention relates to identification between cultivated population and wild population of large yellow croaker, and mitochondrial molecule mark for identification between cultivated population and wild population of large yellow croaker has difference of four bases, including one transversion and three transformatiions. The identification process includes the steps of: designing and synthesizing one pair of conservative primers L14841, 5'-CCATCCAACATCTCAGCATGATGAAA-3' and H15149, 5'-GCCCCTCAGAATGATATTTGTCCTCA-3'; PCR amplification of cytochrome gene B(cyt b) in the mitochondrial DNA of both cultivated population and wild population; purification of amplification result; and sequencing and comparing the sequencing result. The identification method is simple.

Description

technical field [0001] The invention relates to an animal marker, in particular to a mitochondrial molecular marker of large yellow croaker and used for distinguishing large yellow croaker breeding populations from wild populations. Background technique [0002] Currently, there is no formal report on the methods used to identify wild and farmed large yellow croakers. Fishermen generally judge whether large yellow croakers are farmed or wild based on their experience such as body color and eye diameter. Generally, consumers judge according to the price, wild ones range from 120 to 260 yuan per catty, and farmed ones cost about 20 yuan per catty. Because experience often makes mistakes and prices are cheated, these two methods cannot be used as reliable standards for distinguishing farmed and wild large yellow croakers. Especially in the case of disputes, a stable and reliable identification method is needed. Contents of the invention [0003] The purpose of the present i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
Inventor 苏永全王德祥王军丁少雄覃映雪
Owner XIAMEN UNIV
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