Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Establishing method for finger print of Angelica sinensis and ligusticum wallichii

A technique for fingerprinting and establishing methods, which is applied in the direction of material separation, analyzing materials, measuring devices, etc., can solve the problem of no report on fingerprint research, and achieve the effects of reducing risks, simple and feasible detection methods, and high precision

Inactive Publication Date: 2011-06-22
SHENYANG PHARMA UNIVERSITY
View PDF1 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is no report on the HPLC fingerprint of Angelica sinensis and its compound preparations

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Establishing method for finger print of Angelica sinensis and ligusticum wallichii
  • Establishing method for finger print of Angelica sinensis and ligusticum wallichii
  • Establishing method for finger print of Angelica sinensis and ligusticum wallichii

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] The establishment of implementation example 1 angelica standard fingerprint

[0039] 1. Instruments and reagents

[0040] SPD-M10A VP High Performance Liquid Chromatograph (Shimadzu Corporation); SPD-M10A VP Diode Array Detector (Shimadzu Corporation); CTO-10AS VP Column Thermostat (Shimadzu Corporation); 98-1-B Type electronic thermostat heating mantle (Tianjin Test Instrument Co., Ltd.); FW-100 high-speed universal grinder (Tianjin Test Instrument Co., Ltd.).

[0041] Ligustilide reference substance (self-made, purity over 98%); Angelica decoction pieces purchased from Tianjin Traditional Chinese Medicine Decoction Pieces Factory; methanol is chromatographically pure, and other reagents are analytically pure.

[0042] 2. Chromatographic conditions

[0043] Chromatographic column: ODS C 18 Chromatographic analysis column (4.6×200mm, 5μm, Dima Company); mobile phase A: methanol-HOAC (99.6:0.4), mobile phase B; water-HOAC (99.6:0.4); gradient elution, the elution prog...

Embodiment 2

[0062] Implementation Example 2 Establishment of Standard Fingerprint of Rhizoma Chuanxiong

[0063] Based on the establishment of the fingerprints of Angelica medicinal materials in Implementation Example 1, the standard fingerprints of Rhizoma Chuanxiong medicinal materials were established. The instruments and reagents, chromatographic conditions, and fingerprint preparation methods were all the same as in Example 1. The precision inspection and reproducibility inspection of the method all meet the technical requirements of fingerprint research, and the solution is stable within 24 hours. The difference from Example 1 lies in the determination of common peaks. Through the mensuration of 10 batches of Rhizoma Chuanxiong medicinal material fingerprint chromatograms, compare its chromatogram, determine total peak 17, take No. 16 peak (ligustilide peak) as reference thing and exceed total peak area 10% common peak is No. 6 peak (A Ferulic acid, RT 0.373), No. 12 peak (RT 0.689...

Embodiment 3

[0069] Implementation Example 3 Establishment of Standard Fingerprint of Compound Angelica Chuanxiong Injection

[0070] 1. Instruments and reagents

[0071] SPD-M10A VP High Performance Liquid Chromatograph (Shimadzu Corporation); SPD-M10A VP Diode Array Detector (Shimadzu Corporation); CTO-10AS VP Column Thermostat (Shimadzu Corporation); 98-1-B Type electronic thermostat heating mantle (Tianjin Test Instrument Co., Ltd.); FW-100 high-speed universal pulverizer (Tianjin Test Instrument Co., Ltd.).

[0072] Ligustilide reference substance (self-made, purity above 98%); Angelica Chuanxiong decoction pieces were purchased from Tianjin Traditional Chinese Medicine Decoction Factory; Chromatographically pure, the rest of the reagents were analytically pure.

[0073] 2. Chromatographic conditions

[0074] Chromatographic column: ODS C 18 Chromatographic analysis column (4.6 × 200mm, 5 μm, Dima Company); mobile phase A: methanol-HOAC (99.6: 0.4), mobile phase B: water-HOAC (99....

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The present invention relates to fingerprint establishing method and standard fingerprint of angelica and Chuanxiong rhizome and their compound preparation. The standard fingerprint establishing method includes: extracting angelica and / or Chuanxiong rhizome with methanol or anhydrous ethanol, dissolving or diluting to proper concentration, high efficiency liquid chromatography detection, recording chromatogram map, and calculating relative retention time and relative peak area of the fingerprint peaks by referring to chromatograph peaks of ligustilide to establish the standard fingerprint. The present invention has the advantages of simultaneous measurement of two effective parts including water soluble and volatile oil in angelica and Chuanxiong rhizome and their compound preparation, and being scientific, simple, stable, accurate and high in repeatability.

Description

technical field [0001] The present invention relates to a research on fingerprints by high performance liquid chromatography (HPLC), to establish standard fingerprints of Chinese medicinal materials Angelica sinensis and Rhizoma Chuanxiong and preparations prepared by them alone or in combination, and to verify the authenticity of Chinese medicinal materials and preparations. and quality monitoring. Background technique [0002] Angelica sinensis is the dried root of Umbelliferae Angelica sinensis (Oliv.) Diels, and Chuanxiong is the dried rhizome of Umbelliferae Ligusticum chuanxiong Hort. The combined use of the two is called bergamot powder, which is published in "Thousand Gold Prescriptions", the name is "Yifang Leiju". They complement each other, promote blood circulation, nourish blood, and promote qi. It can control the dryness of Chuanxiong, and the dryness of Chuanxiong can control the greasyness of Angelica, dispel phlegm without hurting qi and blood, nourish bloo...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/06G01N30/02
Inventor 崔福德冯丽莉胡杰
Owner SHENYANG PHARMA UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com