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Process for preparing poly n-butyl cyan acrylate nanometer particle

A technology of n-butyl cyanoacrylate and nanoparticles, which can be used in medical preparations of non-active ingredients, gene therapy, pharmaceutical formulations, etc., can solve problems such as insurmountable limitations, and achieve high in vitro gene transfer efficiency and protection The effect of avoiding damage and optimizing process conditions

Inactive Publication Date: 2006-02-15
张阳德
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Problems solved by technology

At present, the viral vectors and non-viral vectors commonly used in the rese

Method used

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Embodiment Construction

[0006] The present invention will be described in detail below in conjunction with the embodiments.

[0007] Dissolve Tween 80 in an appropriate amount of double-distilled water, and adjust the pH value of the solution to below 2.8 with 0.1N HCl. Under the action of a constant temperature magnetic stirrer, slowly add n-butyl α-cyanoacrylate monomer into the solution, and continue stirring for 5 hours at room temperature. Centrifuge at room temperature and 5000 rpm for 15 minutes, take the supernatant, and filter it with a filter membrane with a diameter of 0.22 μm, and the product is PBCA-NP. Centrifuge at 40,000 rpm for 1 hour, remove the supernatant, wash twice with double distilled water, weigh after drying, and resuspend the precipitate with an appropriate amount of double distilled water to prepare a PBCA-NP colloidal storage solution.

[0008] Dilute the PBCA-NP colloidal stock solution into a 0.625% aqueous solution. Under the action of a magnetic stirrer, take 16.67ml...

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Abstract

The invention provides a process for preparing polybutylcyanoacrylate nanoparticles (CTAB-PBCA-NP), which comprises, dissolving Twain-80 into right amount of water, stirring at constant temperature, charging alpha-butylcyanoacrylate monomer, centrifuging, filtering the supernatant fluid, removing supernatant fluid, obtaining PBCA-NP colloid storage liquid and diluting, charging into aqueous solution of cetyltrimethyl-ammonium bromide (CTAB), stirring at room temperature, centrifuging to remove the supernatant fluid, finally drying.

Description

technical field [0001] The invention relates to a method for preparing liver cancer gene therapy drug nanoparticles, in particular to the preparation of cetyltriethylammonium bromide-modified polycyanoacrylate n-butyl ester nanoparticles (CTAB-PBCA-NP) method. technical background [0002] In recent years, with the maturation of gene transfer technology, the gene therapy of liver cancer has become one of the research hotspots in biological science and clinical medicine. Regulating the specific introduction of suicide genes into liver cancer cells, or achieving specific expression in liver cancer cells, to maximize the killing of liver cancer cells without damaging normal liver cells is the key to determining its curative effect and feasibility. At present, the viral vectors and non-viral vectors commonly used in the research and clinical application of gene therapy have insurmountable limitations. Nanoparticle gene transporter is a non-viral gene ...

Claims

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Application Information

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IPC IPC(8): A61K48/00A61K9/14A61K47/32A61P35/00
CPCA61K9/1635C09D4/00A61P35/00
Inventor 张阳德
Owner 张阳德
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