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modified sio 2 Preparation equipment and preparation method of nanocarrier

A nanocarrier and equipment technology, applied in the field of preparation of SiO2 nanoparticles, can solve the problems of large side effects of drugs and inability to inhibit tumor cell proliferation well, and achieve the effects of large side effects, high in vitro gene transfer efficiency, and simple operation.

Inactive Publication Date: 2017-03-08
雷世泽
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The prepared modified SiO 2 The application of nanocarriers can effectively solve the problem that the existing tumor therapy drugs have large side effects and cannot well inhibit the proliferation of tumor cells

Method used

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  • modified sio <sub>2</sub> Preparation equipment and preparation method of nanocarrier
  • modified sio <sub>2</sub> Preparation equipment and preparation method of nanocarrier
  • modified sio <sub>2</sub> Preparation equipment and preparation method of nanocarrier

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Experimental program
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Effect test

Embodiment 1

[0024] A modified SiO 2Nano-carrier preparation equipment, including a reaction vessel body 1 made of ceramic materials, an ultrasonic transducer 2, a rotor stirring paddle 3 and a high-frequency electromagnetic coil 4; the reaction vessel body 1 is a cylindrical body with a bottom, and the center of the inner bottom surface is A vertical upward support shaft is provided; a container cover 5 is provided on the top of the cylinder; the lower part of the support shaft is a shaft seat 6, the upper part is a shaft rod 7, and there is a shaft seat 6 and the shaft rod 7. The shaft is connected by step transition, and the diameter of the shaft seat 6 is larger than the diameter of the shaft rod 7; the above-mentioned ultrasonic transducer 2 is fixed on the outer bottom surface of the reaction vessel body 1; the above-mentioned rotor stirring paddle 3 is composed of a metal conductor square frame 8 and paddles 9, The surface of the square frame 8 is lined with corrosion-resistant mate...

Embodiment 2

[0033] The resulting modified SiO 2 Nanocarriers can be further cross-linked with negatively charged DNA molecules through chemical coupling, or carry carbonyl-containing anti-tumor drugs or tumor angiogenesis inhibitors, so as to be applied to tumor molecular imaging diagnosis and siRNA interference therapy. See Figure 7 ; The specific steps are: the prepared modified SiO 2 Mix 30mg of nanocarriers and 6mg of anti-tumor LivinlshRNA plasmid in 50mL of double distilled water, stir at 600r / min for 24-48h at room temperature to make it fully react, after standing and aging for 8-10h to precipitate, then wash with double distilled water After cleaning, dry it for 12-20 hours at 20-60°C under a vacuum of 5-10 Pa to obtain modified SiO 2 Nanocarrier-mediated anti-tumor gene therapy system.

[0034] The anti-tumor LivinlshRNA plasmid and modified SiO 2 The mass content ratio of nanocarriers is 1:3.

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Abstract

The invention relates to a device and a method for preparing a modified SiO2 nano-carrier. The device for preparing the modified SiO2 nano-carrier comprises a reaction vessel body made from a ceramic material, an ultrasonic transducer, a rotor stirring paddle and a high-frequency electromagnetic coil; the method for preparing the modified SiO2 nano-carrier comprises the steps of putting SiO2 nanoparticles into double-distilled water of a reaction vessel; evenly mixing by means of electromagnetic stirring, resuspending, adding octadecy trimethyl ammonium bromide (OTAB), carrying out electromagnetic stirring at the room temperature, centrifuging, standing, aging and removing supernatant; washing the product with the double-distilled water, resuspending again, centrifuging, and carrying out freeze drying to obtain the modified SiO2 nano-carrier. The modified SiO2 nano-carrier has good biocompatibility and free from immunogenicity. The prepared modified SiO2 nano-carrier can effectively solve the problems that the existing tumor treating medicines are large in side effects and cannot well inhibit tumor cell proliferation.

Description

technical field [0001] The present invention relates to SiO 2 Nanoparticle carrier preparation technology, especially related to modified SiO 2 Nanocarrier preparation equipment and a SiO modified with octadecyltrimethylammonium bromide 2 A method for preparing nanoparticles; using the preparation equipment can facilitate the preparation of modified SiO 2 Nanocarriers (OTAB-SiO 2 -NP); the modified SiO prepared by this method 2 Nanocarriers can be used in gene therapy for cancer. Background technique [0002] In recent years, with the maturity of gene transfer technology, cancer gene therapy has become one of the research hotspots in biological science and clinical medicine. At present, the viral vectors and non-viral vectors commonly used in gene therapy research and clinical application have insurmountable limitations. With the emergence of nanotechnology, nanoparticle carriers are attracting more and more attention due to their high transfection efficiency, low toxi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K49/00A61K48/00A61K45/00A61K47/04A61K47/18A61K47/54A61P35/00
CPCA61K31/7105A61K45/00A61K47/02A61K47/18A61K49/0002
Inventor 雷世泽
Owner 雷世泽
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