Hyperimmune serum against Vibrio parahaemolyticus, its preparation and inspecotion for astacin pathogenic bacterium

Abstract

A method for preparing high immune serum resisting parahemolytic vibrio uses germ suspension of parahemolytic vibrio to carry out immune on male rabbit and a method of utilizing prepared high immune serum to detect prawn corpus rubrum disease ¿C producing germ includes enveloping standard antigen; blocking enzyme labeled board; making competitive reaction of antiserum on antigen to be tested; making enzyme labeled antibody reaction, color display reaction and ending reaction; and determining absorbance value.

Description

technical field [0001] The present invention relates to a method for detecting the pathogenic bacteria of prawn red body disease, in particular to a preparation method of hyperimmune serum against Vibrio parahaemolyticus, and an enzyme-linked immunosorbent assay method using the prepared hyperimmune serum ( Competition ELISA) method for detecting Vibrio parahaemolyticus in shrimp. Background technique [0002] The red body disease caused by Vibrio parahaemolyticus (V.parahaemolyticus) occurs in all stages of shrimp from juvenile to adult shrimp, with a long course of disease and high cumulative mortality. Because one of the obvious manifestations of the disease in prawns is "red body", in the absence of scientific detection methods, farmers often associate the disease with "Taura syndrome" and other diseases that also have the manifestation of "red body". Confusion and misjudgment are made, and the timing of treatment is delayed due to the inappropriate measures adopted, re...

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This technology has the characteristics of strong specificity and high sensitivity, but the current price of fluor

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