Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

HAb18Gedomab 1 monoclonal antibody and its light and heavy chain variable area genes, coding polypeptide and use

A variable region, gene technology, applied in the field of biotechnology and cell engineering, can solve the problem of no clear pharmacodynamic results and so on

Active Publication Date: 2006-06-07
FOURTH MILITARY MEDICAL UNIVERSITY
View PDF0 Cites 6 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Others, such as endothelin receptor antagonists, prolyl hydroxylase inhibitors, etc. are under research, and there is no clear pharmacodynamic result yet

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • HAb18Gedomab 1 monoclonal antibody and its light and heavy chain variable area genes, coding polypeptide and use
  • HAb18Gedomab 1 monoclonal antibody and its light and heavy chain variable area genes, coding polypeptide and use
  • HAb18Gedomab 1 monoclonal antibody and its light and heavy chain variable area genes, coding polypeptide and use

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0018] 1. Preparation and identification of monoclonal antibody HAb18Gedomab1.

[0019] Immunization method: 8-10 weeks old female BALB / C mice weighing about 18 g (provided by the animal center of our school) were routinely immunized with HAb18G / CD147 extracellular domain protein, and subcutaneously injected at multiple points, 100 μg / mouse , every 2 weeks with the same dose of antigen plus incomplete Freund's adjuvant to boost immunization, intraperitoneal injection, test the titer of polyclonal antibody in mouse serum before cell fusion, and the tail vein of the higher titer will be given a booster immunization once again, the dose is the same as above. After 3 days, the splenocytes of mice immunized with HAb18C / CD147 extracellular domain protein were fused with the myeloma cell line SP2 / 0 obtained by passage in vivo at a ratio of 5:1 under the action of PEG-1000, and the fused cells were incubated in HAT selective medium cultivated in.

[0020] Establishment of hybridoma c...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The present invention immunizes mouse with HAb18G / CD147 extra-cellular region protein to prepare specific hybrid tumor cell strain HAb18Gedomab1, obtain monoclonal antibody HAb18Gedomab1, which can combine specifically to HAb18G / CD147 expressed effectively in human liver fibrosis tissue and liver stellate cell and possesses functions of promoting MMPs secretion and degrades extra-cellular matrix. The antibody may be used in preparing medicine for reversing liver fibrosis and other matrix deposition related diseases. The present invention clones the light and heavy chain variable area genes successively. Several forms of small molecular gene engineering antibody are constituted and expressed based on the genes and gene engineering method, and the polypeptide coded based on the genes is cross-linked with several effect factors, so as to prepare medicine for reversing liver fibrosis and other matrix deposition related diseases.

Description

technical field [0001] The present invention relates to the field of biotechnology and cell engineering, especially monoclonal antibody HAb18Gedomab1 and its heavy chain, light chain variable region genes, polypeptides encoded by the genes, and the above-mentioned monoclonal antibodies, light and heavy chain variable region genes, and polypeptides application. Background technique [0002] Hepatic fibrosis is a common pathological change and a necessary path for the development of various chronic liver diseases to cirrhosis. Chronic liver disease mainly includes various types of viral hepatitis, especially hepatitis C and hepatitis B. There are more than 280 million HbsAg carriers worldwide. my country is a high prevalence area of ​​hepatitis B, with 130 million HbsAg carriers and more than 30 million hepatitis B patients. 25-40% of hepatitis B patients lead to liver cirrhosis and liver cancer, and the death toll is 400,000 per year. It has become a serious disease in my c...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N15/13A61K39/00A61P1/16C07K16/18C12N5/12
Inventor 陈志南邢金良张思河杨向民汪莉叶卉李别虎
Owner FOURTH MILITARY MEDICAL UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com