Pressure device and its application

A pressurization device and pressure technology, applied in pressure vessels, fixed-capacity gas storage tanks, mechanical equipment, etc., can solve the problems that cannot meet the needs of cell screening, the pressure is not easy to maintain stability, and the amount of reagents increases, so as to save culture and The effect of detecting reagents, reducing the amount of cells used, and simple and feasible operation methods

Inactive Publication Date: 2007-07-11
CHENGDU UNIV OF TRADITIONAL CHINESE MEDICINE
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  • Abstract
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Problems solved by technology

However, this device still has some shortcomings: the pressurization operation is cumbersome, multiple culture bottles need to be monitored at the same time, and the pressure between different culture bottles is not easy to maintain stability; the experiment requires a large number of samples, a large number of

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  • Pressure device and its application
  • Pressure device and its application

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Abstract

The invention provides a pressurized device for drug screening, comprising pressure hull constituted by lower shell (10) and shell cover (11), sealing part between contacting faces of lower shell (10) and shell cover (11), and locking mechanism (20) for fixing the shell cover (11) and the lower shell (10) after pressuring. The device in invention can save a mass of cultivating and measuring reagent and save cell dosage so as to decrease the experiment cost greatly. The invention is provided with convenience operation, high reproducibility and strong maneuverability. It can provide exacter figures for clinical drug screening test and basis for estimating drug use correctly. It also provides a new selection for drug screening of cell level by simulating the high pressure in body.

Description

technical field [0001] The invention relates to a pressurizing device, in particular to a pressurizing device for drug screening, and a method and application of using the pressurizing device for drug screening. Background technique [0002] The culture method of retinal nerve cells under normal pressure is relatively mature and stable, which can better reflect the growth state of cells and the effect of drugs on the survival of cells in vitro, but it cannot specifically investigate the effect of drugs on cells cultured under high pressure. Although there are many studies on the ocular hypertension model of whole animals, it is impossible to observe the pathological process of retinal nerve cell damage caused by ocular hypertension. Therefore, it is necessary to establish a method for in vitro high-pressure culture of retinal nerve cells, which is convenient for drug screening at the level of relevant cells. [0003] At present, most of the pressurized culture devices commo...

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Application Information

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IPC IPC(8): F17C1/00
Inventor孟宪丽张艺段俊国盛艳梅张静龙怡曾洁萍
OwnerCHENGDU UNIV OF TRADITIONAL CHINESE MEDICINE