Novel complex-forming proteins

a technology of complexes and proteins, applied in the field of complexes, can solve the problems of unfavorable formation of complexes with foreign protein partners, and the inability to prepare efficacious vaccines in a conventional way,

Inactive Publication Date: 2003-03-20
JEROME VALERIE +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For example, nonspecific, i.e. undesired, formation of complexes with foreign protein partners is, as is known, the main problem of analysis and diagnosis using antibodies.
Undesired formation of complexes with foreign protein partners can also be the cause of side effects in vivo, for example after injection of antibodies.
The possibilities of preparing efficacious vaccines in a conventional way are restricted.

Method used

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  • Novel complex-forming proteins
  • Novel complex-forming proteins
  • Novel complex-forming proteins

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Experimental program
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Embodiment Construction

[0808] Preparation and Testing of an Activator-Responsive Promoter Unit

[0809] An activator-responsive promoter unit was prepared in which an activator subunit A is linked to an activator subunit B (see FIG. 3). The combination is carried out by means of mutated c-jun and mutated c-fos (see FIG. 4). The complex is a transcription factor which activates an actvator-responsive promoter.

[0810] An embodiment of the activator component of the activator-responsive promoter unit according to the invention consists, downstream in succession, of the following different nucleotide sequences:

[0811] Activator Subunit A:

[0812] the promoter of the cyclin A gene (nucleic acids -214 to +100; Zwicker et al., EMBO J. 14,4514 (1995))

[0813] the nuclear localization signal (NLS) of SV40 (SV40 large T, amino acids 126-132; PKKKRKV (SEQ ID NO.: 20), Dingwall et al., TIBS 16, 478 (1991))

[0814] the acidic transactivation domain (TAD) of HSV-1 VP16 (VP16, amino acids 411 to 455; Triezenberg et al., Genes Dev....

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Abstract

The invention relates to a complex of specifically complex-forming proteins which are not naturally occurring, comprising the following components: a) at least one ligand specific for a target structure, b) at least one protein comprising a mutated dimerization domain, the mutated dimerization domain having been derived by mutation of a naturally occurring dimerization domain, it being possible for this mutated dimerization domain to interact specifically with component c) and the component b) being connected covalently to the component a), c) at least one protein comprising a mutated dimerization domain, the mutated dimerization domain having been derived by mutation of a naturally occurring dimerization domain, it being possible for this mutated dimerization domain to interact specifically with component b) and the component c) is linked covalently to the component d), and d) at least one effector. In addition, the invention relates to the use and preparation of these complexes, and to nucleic acid constructs coding for the proteins mentioned and use thereof.

Description

1. BACKROUND OF THE INVENTION[0001] Complex forming proteins are widespread in nature.[0002] Fundamentally, these proteins can be assigned to the following groups:[0003] antibodies which, with their antigen-binding sites, can bind specifically to the corresponding epitope of an antigen (which can also be another antibody)[0004] protein complexes, such as occur, for example, in the activation of the clotting system or of the complement system[0005] ligand receptor interactions of very different types, for example[0006] of antigens or their epitopes with the Tcell or B-cell receptor[0007] of growth factors, cytokines, chemokines, peptide hormones, steroid hormones and mediators with their respective receptors[0008] of enzymes such as, for example, uPA or tPA with its receptor[0009] of virus proteins with their respective cell receptor[0010] adhesions between adhesion molecules[0011] protein complexes in signal transmission, control of the cell cycle and control of the transcription of...

Claims

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Application Information

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IPC IPC(8): A61K31/7115A61K35/76A61K38/00A61K39/00A61K39/002C12N15/09A61K39/12A61K39/395A61K48/00A61P7/00A61P9/10A61P25/28A61P29/00A61P31/04A61P31/12A61P35/00A61P37/02A61P43/00C07K7/08C07K14/00C07K14/82C07K19/00C12N1/19C12N1/21C12N5/10C12N15/10C12N15/12C12N15/62C12P21/02
CPCA61K38/00C07K7/08C07K14/82C07K2319/00C07K2319/09C07K2319/50C07K2319/71C07K2319/73C07K2319/81C12N15/1055C12N15/62A61P25/28A61P29/00A61P31/04A61P31/12A61P35/00A61P37/02A61P43/00A61P7/00A61P9/10C07K19/00
InventorJEROME, VALERIESEDLACEK, HANS-HARALDMUELLER, ROLF
OwnerJEROME VALERIE