Antigen Transduced T Cells Used as a Delivery System for Antigens
a technology of antigen transduction and delivery system, which is applied in the direction of antibody medical ingredients, dsdna viruses, immunological disorders, etc., to achieve the effect of monitoring the effectiveness of vaccination and stimulating the immune response against transgen
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example 1
[0204]The infusion of donor lymphocytes in the context of allogeneic bone marrow transplantation (allo-BMT) for leukaemia is an established strategy to control tumor relapse and viral infections. To circumvent the inherent risk of graft-vs-host disease (GVHD) BM-donor lymphocytes can be transduced by a suicide gene, conferring sensitivity to a drug, that may allow the selective elimination of the transduced cells for both therapeutic and safety issues.
[0205]Peripheral blood mononuclear cells (PBMC) were isolated by Lymphoprep (Nycomed, Oslo, Norvay) density-gradient centrifugation from all the BM-donors and the healthy controls studied.
[0206]Activation. Two different activation signals were used to activate T cells (hereafter referred to as T blasts): phytoemoagglutinine (PHA) 2 μg / ml (Boehringer Mannheim); anti-CD3 mAb (OKT3) 30 ng / ml (Orthoclone, Milan, Italy); OKT3 (30 ng / ml). Activated T cells were cultured at 1×106 cells / ml, in RPMI 1640 supplemented with 5% autologous serum in...
example 2
[0213]Further studies were carried out to identify the target antigens of the immune response. Effector cells derived from one of the patient were tested against autologous T blasts expressing single components of the vector. T blasts expressing HSV-TK were lysed while target cells expressing the ΔLNGFr were not recognized at all (FIG. 2). Analogous results were obtained with effector cells isolated from other patients.
example 3
[0214]The results from Example 1 (i.e. generation of HSV-TK-specific immunity) were analyzed in relation to the time of the infusions and the number of engineered T cells infused. Time of the infusions, measured as months from bone marrow transplantation (abscissa) and number of engineered T cells injected (ordinate), are presented. The development of an immune response against the transduced cells was evaluated by ex vivo stimulation of PBLs against autologous-transduced and allogeneic T cells as described in example 1. In the presence of a positive anti-allo immune response the patient was considered not immunized (empty symbols) or immunized (full symbols) to the transgene depending on the presence of a cytolytic activity against the transduced cells. Data points represent the cell infusion responsible (full symbols) or not responsible (empty symbols) for the induction of the immune response. For patient UPN-15 two different time points are represented. These results suggested th...
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