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Detection And Quantification Of Acetylamantadine In Urine Samples

a technology of acetylamantadine and urine samples, which is applied in the direction of material magnetic variables, instruments, diagnostics using spectroscopy, etc., can solve the problems of relatively time-consuming and costly detection and quantification of acetylamantadine using liquid chromatography mass spectrometry, and achieve the effect of low cos

Inactive Publication Date: 2016-02-11
BIOMARK TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

The patent describes a way to detect and measure a chemical called acetylamantadine in a cost-effective way.

Problems solved by technology

However, the detection and quantification of acetylamantadine using liquid chromatography mass spectrometry is relatively time consuming and costly.

Method used

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  • Detection And Quantification Of Acetylamantadine In Urine Samples
  • Detection And Quantification Of Acetylamantadine In Urine Samples
  • Detection And Quantification Of Acetylamantadine In Urine Samples

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Embodiment Construction

[0015]Disclosed herein is the use of Raman spectroscopy to identify and quantify acetylamantadine, a product of spermidine / spermine N1-acetyltransferase (SSAT) metabolism, in urine samples. Urine is a concentrated solution of many salts, polar metabolites and multiple non-polar steroids. Expected concentration of acetylamantadine is about 1000 times smaller than that of amantadine in urine samples. The distinction between amantadine and acetylamantadine can be based on the vibrational band of a carbonyl group at an approximately 1600 cm−1 wavenumber. There are a few other differences between the spectra of amantadine and acetylamantadine, but this Raman band may be of particular interest as it is present only in the spectrum of acetylamantadine.

[0016]A urine sample was prepared and different constituents of the urine sample were separated using solid phase extraction (SPE). The urine sample is accordingly pre-treated using solid phase extraction to remove impurities prior to using R...

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Abstract

A method for quantifying acetylamantadine in a urine sample comprises eluting acetylamantadine from the urine sample using solid phase extraction and quantifying the acetylamantadine eluted from the urine sample using Raman spectroscopy.

Description

BACKGROUND OF THE INVENTION[0001]1. Field of the Invention[0002]The present invention relates to the detection and quantification of biomarkers and, in particular, to the detection and quantification of acetylamantadine in urine samples.[0003]2. Description of the Related Art[0004]Liquid chromatography mass spectrometry has been successfully employed to detect and quantify extremely low concentrations of acetylamantadine in biological samples such as urine. This may facilitate the diagnosis of cancer at an early stage as the quantification of acetylated forms of spermidine / spermine N1-acetyltransferase (SSAT) including amantadine may be used to detect various pathological conditions including cancer as disclosed in U.S. Pat. No. 6,811,967 which issued to Sitar et al. on Nov. 4, 2004, and the full disclosure of which is incorporated herein by reference. However, the detection and quantification of acetylamantadine using liquid chromatography mass spectrometry is relatively time consu...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): G01N21/65G01N33/493
CPCG01N21/658G01N33/493G01N1/4055A61B5/0075C12Q1/48G01N15/0205G01N15/1434G01N21/65G01N27/44721G01N27/74G01N33/574G01N33/57484
Inventor GORDON, REUVENCHENG, BRIANBUX, RASHIDRAMJIAWAN, BRAMAHMED, AFTABHOF, FRASER ALAN
Owner BIOMARK TECH