Two-dimensional liquid chromatographic analyzer

Abstract

A two-dimensional liquid chromatographic analyzer, in which a temperature control part changes a temperature in a single-step manner and switches the temperature at a high speed. Also, the temperature control part has a holder around a separation column so that the temperature of the separation column can be changed to a preset temperature. A first separation column thereof changes elution time(s) of the objective component(s) by temperature modulation and the mobile phase of the first separation column is an aqueous mobile phase having a constant composition.

Description

RELATED APPLICATIONS[0001]This application is a divisional patent application of U.S. application Ser. No. 15 / 081,103, filed Mar. 25, 2016, which claims priority under 35 U.S.C. § 119 to Japanese Patent Application Nos. 2015-080384 filed Mar. 25, 2015 and 2016-79130 filed Mar. 25, 2016, the entire contents of which are hereby incorporated by reference.BACKGROUND OF THE INVENTION1. Field of the Invention[0002]The disclosure relates to a liquid chromatograph. Specifically, the disclosure relates to a two-dimensional liquid chromatographic analyzer having a plurality of separation columns and an analytical method.2. Description of the Related Art[0003]A liquid chromatograph is an apparatus configured to separate objective components in a sample by a separation column while feeding a mobile phase and to detect the components that flow out in an order of their separation by a detector such as a spectrophotometer so as to analyze the components of the sample.[0004]For the liquid chromatog...

AI Technical Summary

Benefits of technology

[0020]The disclosure provides a two-dimensional liquid chromatographic analyzer and an analytical method, which are capable of performing high sensitive analysis of objective component(s) in a sample such as a biological one with a simple configuration in which a system for feeding a mobile phase for desalination is not needed by preventing the salt precipitation.

[0025]When the present analyzer is used, it becomes possible to analyze components of a biological sample or the like with a simple system.

[0026]By adopting a temperature-responsible gel modification separation column as a first-dimensional first separation column, it is possible to separate major contaminant components and a drug group in a biological sample such as serum with an aqueous mobile phase alone.

[0027]The temperature-responsible gel modification separation column can control elution time of a drug in the first-dimensional first separation column by temperature control. By using the temperature-responsible gel modification separation column, at the time when a plurality of drugs are simultaneously analyzed, the elution time of the drug group can be controlled by temperature modulation and thus any drug can be introduced into a second-dimensional second separation column.

[0028]The mobile phase of the first-dimensional first separation column is an aqueous mobile phase having a constant composition and it is not necessary to use a buffer solution from which a salt may be precipitated through mixing with a mobile phase of the second separation column containing an organic solvent at the introduction into the second-dimensional column. Therefore, it is unnecessary to feed a mobile phase for desalination and a trap column and a switching valve become unnecessary, so that system configuration becomes simple. The simple system configuration also improves operability and maintainability.

[0032]Thereby, in the second-dimensional chromatogram, the peak(s) of the objective component(s) becomes sharp and thus high sensitivity analysis is possible.

Problems solved by technology

In order to prevent the clogging, a trap column for feeding a mobile phase for desalination becomes necessary and there is a problem that system configuration is complicated by providing a switching valve for the trap column and the like.

Images