Analytical method of ecological effect influence of domestic-garbage-leachate-contaminated soil

Abstract

The invention relates to an analytical method of ecological effect influence of domestic-garbage-leachate-contaminated soil, comprising the following steps: 1. adjusting moisture contents of a contaminated soil sample and an uncontaminated soil sample to be 60% of field moisture capacity and culturing at the temperature of 20-30 DEG C; 2. selecting microbial activity indexes (respiratory intensity and soil enzymatic activity(catalase, amylase, urease and invertase)) and biotoxicity indexes (protozoon fatality rate) as ecological effect indexes of a soil sample; 3. comparing according to the difference of ecological indicators of the contaminated soil sample and the uncontaminated soil sample and analyzing the contaminated degree of the soil by the domestic garbage leachate. Indexes monitoring referred in the analytical method disclosed by the invention is convenient to operate and is easy to carry out, and can be a supplement for chemical index monitoring.

Description

technical field

[0001] The invention belongs to the technical field of solid waste treatment, and relates to a method for analyzing the ecological effect of domestic garbage leachate polluted soil. Background technique

[0002] At present, there are a large number of simple domestic waste dumps, which will pollute the surrounding soil and groundwater due to the lack of effective anti-seepage measures. Some of the following problems in domestic waste sanitary landfills can also cause leachate leakage: the anti-seepage lining is damaged due to mechanical and natural effects, resulting in leachate leakage; the collection and drainage system of leachate is corroded and loses its function , leading to leachate leakage; the monitoring system of the landfill site is difficult to monitor the leachate leakage in the early stage, and the pollution caused cannot be controlled in time.

[0003] Leakage of household garbage leachate will inevitably cause serious soil pollution, and dome...

Examples

example 1

[0025] A site polluted by domestic waste leachate in a certain place was selected as the analysis object. After removing the top 10cm of soil, dig out 4-5kg of soil and put it in a sealed plastic bag or bucket for later use. Soil samples were air-dried at room temperature to constant weight, crushed manually or mechanically, and then sieved through a 10-mesh sieve. The control soil samples were treated in the same way. Take 1kg of soil for each sample and put it in a glass or plastic container. Make 3 parallel samples of the object soil and the control soil. Adjust the moisture content of each sample soil to 60% of the field water holding capacity, and cultivate it at a constant temperature at 25°C for 2-3 days. Perform indicator detection. The soil microbial activity (respiration intensity and soil enzyme activity (catalase, amylase, urease and sucrase)) and biological toxicity (protozoan lethality) of soil samples were detected. The protozoan adopts Tetrahymena piriformis,...

example 2

[0031] The sampling and testing methods are the same as in Example 1, wherein the protozoan is Pseudonephron moschii, the constant temperature culture temperature is 20° C., and the mixing ratio of medium and supernatant in the mixed liquid is 1:15.

[0032] The monitoring data are as follows:

[0033]

[0034] Note: The unit of respiratory intensity is mgCO 2 / (g soil h), the unit of catalase activity is ml (0.1mol KMnO 4 ) g -1 (soil), amylase activity unit is mg (glucose) g -1 (soil), the unit of urease activity is mg (NH 3 -N)·g -1 (soil), the sucrase activity unit is ml (0.1mol Na 2 S 2 o 3 ) g -1 (soil), the unit of protozoan lethality is %.

[0035] According to the rules of the analysis provided in step 3, it is considered that the leachate pollution has caused serious adverse ecological effects.

example 3

[0037] Sampling and testing methods are the same as in Example 1, wherein the protozoan adopts dilated nephrite, the constant temperature culture temperature is 25° C., and the mixing ratio of medium and supernatant in the mixed liquid is 1:20.

[0038] The monitoring data are as follows:

[0039]

[0040] Note: The unit of respiratory intensity is mgCO 2 / (g soil h), the unit of catalase activity is ml (0.1mol KMnO 4 ) g -1 (soil), amylase activity unit is mg (glucose) g -1 (soil), the unit of urease activity is mg (NH 3 -N)·g -1 (soil), the sucrase activity unit is ml (0.1mol Na 2 S2 o 3 ) g -1 (soil), the unit of protozoan lethality is %.

[0041] Following the rules of the analysis provided in Step 3, no adverse ecological effects from leachate contamination were considered.

technical field

[0001] The invention belongs to the technical field of solid waste treatment, and relates to a method for analyzing the ecological effect of domestic garbage leachate polluted soil. Background technique

[0002] At present, there are a large number of simple domestic waste dumps, which will pollute the surrounding soil and groundwater due to the lack of effective anti-seepage measures. Some of the following problems in domestic waste sanitary landfills can also cause leachate leakage: the anti-seepage lining is damaged due to mechanical and natural effects, resulting in leachate leakage; the collection and drainage system of leachate is corroded and loses its function , leading to leachate leakage; the monitoring system of the landfill site is difficult to monitor the leachate leakage in the early stage, and the pollution caused cannot be controlled in time.

[0003] Leakage of household garbage leachate will inevitably cause serious soil pollution, and dome...

Examples

example 1

[0025] A site polluted by domestic waste leachate in a certain place was selected as the analysis object. After removing the top 10cm of soil, dig out 4-5kg of soil and put it in a sealed plastic bag or bucket for later use. Soil samples were air-dried at room temperature to constant weight, crushed manually or mechanically, and then sieved through a 10-mesh sieve. The control soil samples were treated in the same way. Take 1kg of soil for each sample and put it in a glass or plastic container. Make 3 parallel samples of the object soil and the control soil. Adjust the moisture content of each sample soil to 60% of the field water holding capacity, and cultivate it at a constant temperature at 25°C for 2-3 days. Perform indicator detection. The soil microbial activity (respiration intensity and soil enzyme activity (catalase, amylase, urease and sucrase)) and biological toxicity (protozoan lethality) of soil samples were detected. The protozoan adopts Tetrahymena piriformis,...

example 2

[0031] The sampling and testing methods are the same as in Example 1, wherein the protozoan is Pseudonephron moschii, the constant temperature culture temperature is 20° C., and the mixing ratio of medium and supernatant in the mixed liquid is 1:15.

[0032] The monitoring data are as follows:

[0033]

[0034] Note: The unit of respiratory intensity is mgCO 2 / (g soil h), the unit of catalase activity is ml (0.1mol KMnO 4 ) g -1 (soil), amylase activity unit is mg (glucose) g -1 (soil), the unit of urease activity is mg (NH 3 -N)·g -1 (soil), the sucrase activity unit is ml (0.1mol Na 2 S 2 o 3 ) g -1 (soil), the unit of protozoan lethality is %.

[0035] According to the rules of the analysis provided in step 3, it is considered that the leachate pollution has caused serious adverse ecological effects.

example 3

[0037] Sampling and testing methods are the same as in Example 1, wherein the protozoan adopts dilated nephrite, the constant temperature culture temperature is 25° C., and the mixing ratio of medium and supernatant in the mixed liquid is 1:20.

[0038] The monitoring data are as follows:

[0039]

[0040] Note: The unit of respiratory intensity is mgCO 2 / (g soil h), the unit of catalase activity is ml (0.1mol KMnO 4 ) g -1 (soil), amylase activity unit is mg (glucose) g -1 (soil), the unit of urease activity is mg (NH 3 -N)·g -1 (soil), the sucrase activity unit is ml (0.1mol Na 2 S2 o 3 ) g -1 (soil), the unit of protozoan lethality is %.

[0041] Following the rules of the analysis provided in Step 3, no adverse ecological effects from leachate contamination were considered.

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