Artificial dermis bracket and preparation method of artificial dermis bracket

Abstract

The invention relates to an artificial dermis bracket. The artificial dermis bracket comprises a three-dimensional porous structure with a deep layer and a shallow layer, wherein sustained release microspheres loaded with functional factors are introduced into the artificial dermis bracket, and comprise microspheres loaded with antibiotics and microspheres loaded with skin growth factors, and a gradient structure is formed by the three-dimensional porous structure of the artificial dermis bracket due to different porosities of the deep layer and the shallow layer. The preparation method of the artificial dermis bracket mainly comprises the steps of: preparing the sustained release microspheres loaded with the functional factors; preparing collagen solutions with two concentrations as matrix solutions; and mixing the sustained release microspheres loaded with the functional factors into the corresponding matrix solutions and molding the three-dimensional porous structure comprising the deep layer and the shallow layer so as to form the artificial dermis bracket with introduction of the sustained release microspheres loaded with the functional factors. The artificial dermis bracket provided by the invention has antibacterial activity and the property of promoting wound healing.

Description

technical field

[0001] The invention belongs to the fields of biomedical material technology and biomedical engineering. More precisely, the present invention relates to an artificial dermal scaffold and its preparation method. technical background

[0002] As a human organ, the skin has the functions of retaining moisture, breathing and preventing bacterial invasion. When the skin is damaged by trauma, burns, inflammation, etc., especially when a large area of ​​skin is seriously damaged, the wound should be protected immediately. If only a superficial or small area of ​​skin is damaged, new skin will regenerate itself. If a large area of ​​deep skin is traumatized, the skin cannot repair itself, and autologous skin grafting, such as stamp skin grafting, mesh skin grafting, and skin inlays, is usually required. Or use artificial skin products. The artificial skin products in the prior art all have insufficient ability of the skin to induce autologous skin tissue repair,...

Examples

preparation example Construction

[0030] It can be understood that, as a modification of the above-mentioned embodiment, the two-factor slow-release microspheres (slow-release antibiotics and slow-release skin growth factors) can also be selectively loaded on either the superficial layer or the deep layer, or loaded on the same layer. level. The steps mainly included in the preparation method of the artificial dermal scaffold of the present invention are specifically described as follows.

[0031] Step 1: Prepare slow-release microspheres loaded with functional factors, which may include but not limited to microspheres loaded with antibiotics and / or microspheres loaded with skin factors.

[0032]Among them, in the process example of preparing antibiotic-loaded microspheres in step 1, first take 0.4 mL of an ultrapure aqueous solution of 10-200 mg / mL antibiotics such as gentamicin or vancomycin or penicillin to 4 mL of PLA or PLGA or PGA Dichloromethane DCM solution with a concentration of 20~200mg / mL is emuls...

example 1

[0042] A preparation method of a two-factor slow-release artificial dermis support is:

[0043] First, prepare antibiotic-loaded PLGA microspheres: take 0.4 mL of 10 mg / mL ultrapure aqueous solution of gentamycin to 4 mL of PLGA DCM solution, emulsify with an internal cutting homogenizer at a speed of 3000 rpm for 30 seconds, and prepare To obtain the inner emulsion (w / o); in 30mL of deionized water, add 0.3mL polysorbate-80, mechanically stir until it is uniformly dispersed, and obtain the outer aqueous phase; add the inner emulsion to the outer aqueous phase, and homogenize The double emulsion (w / o / w) was obtained by emulsifying at a speed of 3000 rpm for 30 seconds with a paddle machine; the double emulsion was stirred at a medium speed for 3 hours until the DCM was volatilized, and then left to stand overnight. After centrifugation, wash with water three times to obtain the suspension of PLGA microspheres loaded with antibiotics;

[0044] At the same time, prepare microsp...

example 2

[0054] A preparation method of a two-factor slow-release artificial dermis support is:

[0055] First, prepare antibiotic-loaded PLGA microspheres: take 0.4 mL of 200 mg / mL penicillin ultrapure aqueous solution to 4 mL of PGA DCM solution, and emulsify with an internal cutting homogenizer at a speed of 5000 rpm for 60 seconds to obtain an inner emulsion (w / o); in 30mL deionized water, add 0.3mL polysorbate-80, mechanically stir until uniformly dispersed, and obtain the outer water phase; add the inner emulsion into the outer water phase, and use an internal cutting type homogenizer to The double emulsion (w / o / w) was prepared by emulsifying at 8000 rpm for 90 seconds; the double emulsion was stirred at a medium speed for 3 hours until the DCM was volatilized and left to stand overnight. After centrifugation, wash with water three times to obtain the PGA microsphere suspension loaded with penicillin;

[0056] At the same time, prepare microspheres loaded with skin factors: take...

technical field

[0001] The invention belongs to the fields of biomedical material technology and biomedical engineering. More precisely, the present invention relates to an artificial dermal scaffold and its preparation method. technical background

[0002] As a human organ, the skin has the functions of retaining moisture, breathing and preventing bacterial invasion. When the skin is damaged by trauma, burns, inflammation, etc., especially when a large area of ​​skin is seriously damaged, the wound should be protected immediately. If only a superficial or small area of ​​skin is damaged, new skin will regenerate itself. If a large area of ​​deep skin is traumatized, the skin cannot repair itself, and autologous skin grafting, such as stamp skin grafting, mesh skin grafting, and skin inlays, is usually required. Or use artificial skin products. The artificial skin products in the prior art all have insufficient ability of the skin to induce autologous skin tissue repair,...

Examples

preparation example Construction

[0030] It can be understood that, as a modification of the above-mentioned embodiment, the two-factor slow-release microspheres (slow-release antibiotics and slow-release skin growth factors) can also be selectively loaded on either the superficial layer or the deep layer, or loaded on the same layer. level. The steps mainly included in the preparation method of the artificial dermal scaffold of the present invention are specifically described as follows.

[0031] Step 1: Prepare slow-release microspheres loaded with functional factors, which may include but not limited to microspheres loaded with antibiotics and / or microspheres loaded with skin factors.

[0032]Among them, in the process example of preparing antibiotic-loaded microspheres in step 1, first take 0.4 mL of an ultrapure aqueous solution of 10-200 mg / mL antibiotics such as gentamicin or vancomycin or penicillin to 4 mL of PLA or PLGA or PGA Dichloromethane DCM solution with a concentration of 20~200mg / mL is emuls...

example 1

[0042] A preparation method of a two-factor slow-release artificial dermis support is:

[0043] First, prepare antibiotic-loaded PLGA microspheres: take 0.4 mL of 10 mg / mL ultrapure aqueous solution of gentamycin to 4 mL of PLGA DCM solution, emulsify with an internal cutting homogenizer at a speed of 3000 rpm for 30 seconds, and prepare To obtain the inner emulsion (w / o); in 30mL of deionized water, add 0.3mL polysorbate-80, mechanically stir until it is uniformly dispersed, and obtain the outer aqueous phase; add the inner emulsion to the outer aqueous phase, and homogenize The double emulsion (w / o / w) was obtained by emulsifying at a speed of 3000 rpm for 30 seconds with a paddle machine; the double emulsion was stirred at a medium speed for 3 hours until the DCM was volatilized, and then left to stand overnight. After centrifugation, wash with water three times to obtain the suspension of PLGA microspheres loaded with antibiotics;

[0044] At the same time, prepare microsp...

example 2

[0054] A preparation method of a two-factor slow-release artificial dermis support is:

[0055] First, prepare antibiotic-loaded PLGA microspheres: take 0.4 mL of 200 mg / mL penicillin ultrapure aqueous solution to 4 mL of PGA DCM solution, and emulsify with an internal cutting homogenizer at a speed of 5000 rpm for 60 seconds to obtain an inner emulsion (w / o); in 30mL deionized water, add 0.3mL polysorbate-80, mechanically stir until uniformly dispersed, and obtain the outer water phase; add the inner emulsion into the outer water phase, and use an internal cutting type homogenizer to The double emulsion (w / o / w) was prepared by emulsifying at 8000 rpm for 90 seconds; the double emulsion was stirred at a medium speed for 3 hours until the DCM was volatilized and left to stand overnight. After centrifugation, wash with water three times to obtain the PGA microsphere suspension loaded with penicillin;

[0056] At the same time, prepare microspheres loaded with skin factors: take...