A microbial preparation with triple effects of water purification, fertilizer and health care and its preparation method

Abstract

The invention relates to a microbial preparation with efficacy of purifying water, fertilizing water and caring health and a preparation method thereof. The composite microbial preparation is prepared by respectively performing fermentation on lactobacillus acidophilus, candida utilis and rhodopseudomonas palustris and combining the three fermentation products. The culture cost of aquatic-product farmers is reduced and the culture efficiency is improved.

Description

technical field

[0001] The invention relates to an aquatic microbial preparation, which belongs to the field of microbial technology application. Background technique

[0002] With the wide application of pharmaceutical feed additives such as antibiotics, olaquindox, and quinolone in the aquaculture industry, the side effects and disadvantages caused by pathogenic bacteria such as drug resistance, toxicity, and "three causes" (teratogenic, carcinogenic, and mutagenic) also gradually revealed. Residual poison, pollute the environment, and the problem of threatening human health has become a focus of great concern to breeding workers and health workers all over the world. With the widespread application of animal microecological preparations in animal husbandry and other aspects, the application in aquaculture has attracted more and more attention, and its good effect has been confirmed by a large number of experiments and production practices. Microecological preparations h...

Examples

Embodiment 1

[0016] Embodiment 1: produce 10 liters of microbial preparations with clean water provided by the invention, fertilizing water and health care triple effects, prepared according to the following formula and method:

[0017] 1: Insert the Lactobacillus acidophilus strains that have been activated and cultured for 24 hours into a liquid medium prepared at a ratio of 5% coconut water, 1% peptone, 1% yeast extract, and 0.5% sodium chloride, at 37°C After static culture for 72 hours, the amount of Lactobacillus acidophilus detected was 2.6×10 8 cfu / mL;

[0018] 2: Insert the Candida utilis strains that have been activated and cultured for 24 hours into a liquid prepared in proportions of 1% glucose, 1% peptone, 1% yeast extract, 0.5% dipotassium hydrogen phosphate, and 0.085% magnesium sulfate In the culture medium, cultured with shaking at 30°C for 48 hours, the amount of Candida utilis detected was 3.7×10 8 cfu / mL;

[0019] 3: Insert the Rhodopseudomonas palustris strains that...

Embodiment 2

[0024] Embodiment 2: produce 100 liters of microbial preparations with clean water provided by the invention, fertilizing water and health care triple effects, prepared according to the following formula and method:

[0025] 1: Insert the Lactobacillus acidophilus strains that have been activated and cultured for 24 hours into a liquid medium prepared at a ratio of 5% coconut water, 1% peptone, 1% yeast extract, and 0.5% sodium chloride, at 37°C After static culture for 72 hours, the amount of Lactobacillus acidophilus detected was 3.5×10 8 cfu / mL;

[0026] 2: Insert the Candida utilis strains that have been activated and cultured for 24 hours into a liquid prepared in proportions of 1% glucose, 1% peptone, 1% yeast extract, 0.5% dipotassium hydrogen phosphate, and 0.085% magnesium sulfate. In the culture medium, cultured with shaking at 30°C for 48 hours, the amount of Candida utilis detected was 4.2×10 8 cfu / mL;

[0027] 3: Insert the Rhodopseudomonas palustris strains th...

technical field

[0001] The invention relates to an aquatic microbial preparation, which belongs to the field of microbial technology application. Background technique

[0002] With the wide application of pharmaceutical feed additives such as antibiotics, olaquindox, and quinolone in the aquaculture industry, the side effects and disadvantages caused by pathogenic bacteria such as drug resistance, toxicity, and "three causes" (teratogenic, carcinogenic, and mutagenic) also gradually revealed. Residual poison, pollute the environment, and the problem of threatening human health has become a focus of great concern to breeding workers and health workers all over the world. With the widespread application of animal microecological preparations in animal husbandry and other aspects, the application in aquaculture has attracted more and more attention, and its good effect has been confirmed by a large number of experiments and production practices. Microecological preparations h...

Examples

Embodiment 1

[0016] Embodiment 1: produce 10 liters of microbial preparations with clean water provided by the invention, fertilizing water and health care triple effects, prepared according to the following formula and method:

[0017] 1: Insert the Lactobacillus acidophilus strains that have been activated and cultured for 24 hours into a liquid medium prepared at a ratio of 5% coconut water, 1% peptone, 1% yeast extract, and 0.5% sodium chloride, at 37°C After static culture for 72 hours, the amount of Lactobacillus acidophilus detected was 2.6×10 8 cfu / mL;

[0018] 2: Insert the Candida utilis strains that have been activated and cultured for 24 hours into a liquid prepared in proportions of 1% glucose, 1% peptone, 1% yeast extract, 0.5% dipotassium hydrogen phosphate, and 0.085% magnesium sulfate In the culture medium, cultured with shaking at 30°C for 48 hours, the amount of Candida utilis detected was 3.7×10 8 cfu / mL;

[0019] 3: Insert the Rhodopseudomonas palustris strains that...

Embodiment 2

[0024] Embodiment 2: produce 100 liters of microbial preparations with clean water provided by the invention, fertilizing water and health care triple effects, prepared according to the following formula and method:

[0025] 1: Insert the Lactobacillus acidophilus strains that have been activated and cultured for 24 hours into a liquid medium prepared at a ratio of 5% coconut water, 1% peptone, 1% yeast extract, and 0.5% sodium chloride, at 37°C After static culture for 72 hours, the amount of Lactobacillus acidophilus detected was 3.5×10 8 cfu / mL;

[0026] 2: Insert the Candida utilis strains that have been activated and cultured for 24 hours into a liquid prepared in proportions of 1% glucose, 1% peptone, 1% yeast extract, 0.5% dipotassium hydrogen phosphate, and 0.085% magnesium sulfate. In the culture medium, cultured with shaking at 30°C for 48 hours, the amount of Candida utilis detected was 4.2×10 8 cfu / mL;

[0027] 3: Insert the Rhodopseudomonas palustris strains th...

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