Alpha-amylase inhibitor and extraction method and application thereof

A technology of amylase inhibitor and extraction method, which is applied in the field of medicine, can solve the side effects of chemical drugs and other problems, and achieve the effects of promoting body weight, hindering digestion and absorption, and reducing calorie intake

CN105362281AActive Publication Date: 2016-03-02SHENZHEN UNIV
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
Publication Date
2016-03-02

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Abstract

The invention discloses an alpha-amylase inhibitor and an extraction method and application thereof. The alpha-amylase inhibitor is phenylpropanoid glycoside. The general structural formula of phenylpropanoid glycoside is shown in the specification, wherein when R2=H, R1=R3=OH; when R2=rha, R1=R3=OH; when R2=rha, R1=R3=H; when R2=H, R1=OH, and R3=H; when R2=H, R1=R3=H. Phenylpropanoid glycoside of the structure is a natural compound instead of a chemical synthetic. Phenylpropanoid glycoside has a significant inhibiting effect on alpha-amylase, so that the decomposition catalysis of alpha-amylase on starch is effectively inhibited, digestion and absorption of an organism on starch are hindered, calorie intake is reduced, and accordingly weight reduction is promoted. Different from other weight-reducing chemical drugs, phenylpropanoid glycoside does not act on the nervous centralis of the organism and is small in side effect.
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Description

technical field

[0001] The invention relates to the technical field of medicine, in particular to an α-amylase inhibitor and its extraction method and application. Background technique

[0002] Obesity and related diseases will be greatly improved by preventing the digestion and absorption of starch, which is a source of heat. α-amylase can hydrolyze starch. Therefore, research and development of substances that can inhibit α-amylase is an effective way to prevent and treat obesity. The slimming medicines with higher share in the market are mainly some chemical drugs, but the chemical drugs are usually accompanied by certain side effects. Natural products have the advantages of high efficiency, low toxicity, and no side effects. Some plant polyphenolic compounds can inhibit amylase, hinder the body's digestion and absorption of starch, reduce calorie intake, thereby promoting weight loss and reducing carbohydrate intake to a large extent, so amylase inhibitors are helpful ...

Examples

Embodiment

[0030] (1) Extraction of phenylpropanoid glycosides

[0031] Take the dry leaves of Ligustrum adenophorum, mash them, extract them under hot reflux with ethanol, stand and filter, concentrate under reduced pressure to obtain the extract, repeat the operation 3 times, combine the obtained supernatants, and the combined supernatants are subjected to Diaion column chromatography Separated to obtain phenylpropanoid glycoside.

[0032] (2) Determination of enzyme activity

[0033] α-amylase enzyme activity was measured by Bernfeld method, using soluble starch as substrate, taking 0.25 mL of α-amylase solution with a concentration of 2 mg / mL and incubating it in a water bath at 37°C for 10 minutes, then adding 0.5 mL of 1.5% soluble starch After reacting at 37°C for 5 minutes, add 1 mL of 3,5-dinitrosalicylic acid (DNS reagent), heat in a boiling water bath for 10 minutes, cool rapidly, and measure the absorbance at a wavelength of 520 nm.

[0034] Inhibition rate and half inhibit...