A kind of α-amylase inhibitor and its extraction method and application

A technology of amylase inhibitor and extraction method, which is applied in the field of medicine, can solve the side effects of chemical drugs and other problems, and achieve the effects of promoting body weight, hindering digestion and absorption, and reducing calorie intake

CN105362281BActive Publication Date: 2018-06-19SHENZHEN UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Publication Date
2018-06-19

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Abstract

The invention discloses an alpha-amylase inhibitor and an extraction method and application thereof. The alpha-amylase inhibitor is phenylpropanoid glycoside. The general structural formula of phenylpropanoid glycoside is shown in the specification, wherein when R2=H, R1=R3=OH; when R2=rha, R1=R3=OH; when R2=rha, R1=R3=H; when R2=H, R1=OH, and R3=H; when R2=H, R1=R3=H. Phenylpropanoid glycoside of the structure is a natural compound instead of a chemical synthetic. Phenylpropanoid glycoside has a significant inhibiting effect on alpha-amylase, so that the decomposition catalysis of alpha-amylase on starch is effectively inhibited, digestion and absorption of an organism on starch are hindered, calorie intake is reduced, and accordingly weight reduction is promoted. Different from other weight-reducing chemical drugs, phenylpropanoid glycoside does not act on the nervous centralis of the organism and is small in side effect.
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Description

Technical field

[0001] The present invention relates to the technical field of medicine, in particular to an alpha-amylase inhibitor and its extraction method and application. Background technique

[0002] Preventing the digestion and absorption of starch that produces calories will greatly improve obesity and related diseases. α-Amylase can hydrolyze starch. Therefore, research and development of substances that inhibit α-amylase is an effective way to prevent and treat obesity. The weight-loss drugs with a high market share are mainly chemical drugs, but chemical drugs usually have certain side effects. Natural products have the advantages of high efficiency, low toxicity, and no side effects. Some plant polyphenol compounds can inhibit amylase, hinder the body's digestion and absorption of starch, reduce calorie intake, thereby promoting weight loss and reducing carbohydrate intake to a large extent, so amylase inhibitors are helpful Pharmacological potential for weight los...

Examples

Embodiment

[0030] (1) Extraction of phenylpropanoid glycosides

[0031] Take the dried leaves of Ligustrum lucidum, mash them, extract them under reflux with ethanol, stand still and filter, and concentrate under reduced pressure to obtain the extract. Repeat the operation 3 times. The supernatants are combined, and the combined supernatants are subjected to Diaion column chromatography Separate to obtain phenylpropanoside.

[0032] (2) Determination of enzyme activity

[0033] The enzyme activity of α-amylase was determined by Bernfeld method, soluble starch was used as the substrate, 0.25 mL of a-amylase solution with a concentration of 2 mg / mL was incubated in a 37 ℃ water bath for 10 minutes, and 0.5 mL of 1.5% soluble starch was added. After reacting at 37°C for 5 min, 1mL 3,5-dinitrosalicylic acid (DNS reagent) was added, heated in a boiling water bath for 10 min, cooled rapidly, and the absorbance value was measured at a wavelength of 520nm.

[0034] Inhibition rate and half inhibition r...