Needle mushroom extract and preparation method and application thereof

Abstract

The invention relates to the field of active substances, in particular to a needle mushroom extract and a preparation method and application thereof. The needle mushroom extract prepared through the method provided by the invention mainly comprises polysaccharide, protein, vitamins and mineral substances. In vitro experiments show that the needle mushroom extract provided by the invention can inhibit the activity of in vivo alpha-glycosidase. In vivo experiments show that the needle mushroom extract can obviously reduce the blood glucose of II-type diabetic rats, is used for adjusting lipid metabolism disorder, improving insulin resistance, reducing serum free fatty acid level, increasing hepatic glycogen and muscle glycogen storage and repairing damaged pancreas, and has an obvious effect of treating II-type diabetes.

Description

technical field

[0001] The invention relates to the field of active substances, in particular to an extract of Flammulina velutipes and its preparation method and application. Background technique

[0002] Diabetes mellitus is a chronic comprehensive disease mainly caused by the disorder of glucose metabolism, which is formed by the joint action of genetic and acquired environmental factors. Clinically, hyperglycemia is the main feature. In typical cases, symptoms such as polyuria, polydipsia, polyphagia, and weight loss can occur, namely "three excesses and one deficiency", which is called "diabetes syndrome" in Chinese medicine. With the improvement of living standards, the incidence of diabetes has been increasing year by year, and it has become the third chronic disease that seriously endangers human health after cancer and cardiovascular and cerebrovascular diseases. There are two types of diabetes, that is, type 1, insulin-dependent diabetes, which is caused by autoim...

Examples

Embodiment 1

[0073] The preparation of embodiment 1 Flammulina velutipes extract

[0074] The cleaned Flammulina velutipes was crushed and mixed with water, the ratio of solid to water between Flammulina velutipes and water was 1g:7mL, extracted for 6h in a constant temperature water bath at 90°C, poured out the extract, added 2% activated carbon to decolorize in a water bath at 60°C for 2h, and then used Centrifuge at 4000r / min for 15min, filter with suction, concentrate the extract in a water bath at 60°C to 1 / 4 to 1 / 5 of the original volume, then precipitate with ethanol with a volume fraction of 90%, precipitate overnight at room temperature, and centrifuge to obtain The extract was placed in an evaporating dish and evaporated to dryness on a 60°C water bath to obtain Flammulina velutipes extract. The yield is 25.31%.

Embodiment 2

[0075] The preparation of embodiment 2 Flammulina velutipes extract

[0076] Dry the washed Flammulina velutipes at 60°C, press them into powder, pass through a 300-mesh sieve, and mix with water. After decolorizing 2% activated carbon in a water bath at 45°C for 2h, centrifuge at 4000rpm / min for 15min with a centrifuge, filter with suction, and concentrate the extract in a water bath at 45°C to 1 / 4 to 1 / 5 of the original volume, then wash with 90% ethanol Precipitation, deproteinization by sevag method, dialysis bag dialysis, freeze-drying to obtain Flammulina velutipes extract. The yield is 15.33%.

Embodiment 3

[0077] Example 3 Flammulina velutipes extract in vitro α-glucosidase inhibitory effect

[0078] 1.1 Experimental method

[0079] With 4-nitrophenyl-D-glucopyranoside (PNPG) as the substrate, acarbose as the positive drug, and a 96-well microplate reader as the reaction carrier, the final reaction system is 200uL, as measured in Examples 1-2 Provides the inhibitory activity of the extract against α-glucosidase. Add 40uL sample to 40uL α-glucosidase solution with 300U activity, react at 37°C for 5min, add 20uL 0.5mmol / PNPG solution, react at 37°C for 30min, then use 100uL 0.1mol / LNa 2 CO 3 The solution terminates the reaction, and the absorbance of the solution is measured at a wavelength of 405nm. In the above reaction system, the α-glucosidase with absorbance between 0.2 and 0.4 was screened. The inhibition rate (%) of sample to enzyme activity is calculated according to the following formula:

[0080] Inhibition rate (%) = [A Blank ﹣(A 0 ﹣A)] / A Blank ×100%

[0081] I...

technical field

[0001] The invention relates to the field of active substances, in particular to an extract of Flammulina velutipes and its preparation method and application. Background technique

[0002] Diabetes mellitus is a chronic comprehensive disease mainly caused by the disorder of glucose metabolism, which is formed by the joint action of genetic and acquired environmental factors. Clinically, hyperglycemia is the main feature. In typical cases, symptoms such as polyuria, polydipsia, polyphagia, and weight loss can occur, namely "three excesses and one deficiency", which is called "diabetes syndrome" in Chinese medicine. With the improvement of living standards, the incidence of diabetes has been increasing year by year, and it has become the third chronic disease that seriously endangers human health after cancer and cardiovascular and cerebrovascular diseases. There are two types of diabetes, that is, type 1, insulin-dependent diabetes, which is caused by autoim...

Examples

Embodiment 1

[0073] The preparation of embodiment 1 Flammulina velutipes extract

[0074] The cleaned Flammulina velutipes was crushed and mixed with water, the ratio of solid to water between Flammulina velutipes and water was 1g:7mL, extracted for 6h in a constant temperature water bath at 90°C, poured out the extract, added 2% activated carbon to decolorize in a water bath at 60°C for 2h, and then used Centrifuge at 4000r / min for 15min, filter with suction, concentrate the extract in a water bath at 60°C to 1 / 4 to 1 / 5 of the original volume, then precipitate with ethanol with a volume fraction of 90%, precipitate overnight at room temperature, and centrifuge to obtain The extract was placed in an evaporating dish and evaporated to dryness on a 60°C water bath to obtain Flammulina velutipes extract. The yield is 25.31%.

Embodiment 2

[0075] The preparation of embodiment 2 Flammulina velutipes extract

[0076] Dry the washed Flammulina velutipes at 60°C, press them into powder, pass through a 300-mesh sieve, and mix with water. After decolorizing 2% activated carbon in a water bath at 45°C for 2h, centrifuge at 4000rpm / min for 15min with a centrifuge, filter with suction, and concentrate the extract in a water bath at 45°C to 1 / 4 to 1 / 5 of the original volume, then wash with 90% ethanol Precipitation, deproteinization by sevag method, dialysis bag dialysis, freeze-drying to obtain Flammulina velutipes extract. The yield is 15.33%.

Embodiment 3

[0077] Example 3 Flammulina velutipes extract in vitro α-glucosidase inhibitory effect

[0078] 1.1 Experimental method

[0079] With 4-nitrophenyl-D-glucopyranoside (PNPG) as the substrate, acarbose as the positive drug, and a 96-well microplate reader as the reaction carrier, the final reaction system is 200uL, as measured in Examples 1-2 Provides the inhibitory activity of the extract against α-glucosidase. Add 40uL sample to 40uL α-glucosidase solution with 300U activity, react at 37°C for 5min, add 20uL 0.5mmol / PNPG solution, react at 37°C for 30min, then use 100uL 0.1mol / LNa 2 CO 3 The solution terminates the reaction, and the absorbance of the solution is measured at a wavelength of 405nm. In the above reaction system, the α-glucosidase with absorbance between 0.2 and 0.4 was screened. The inhibition rate (%) of sample to enzyme activity is calculated according to the following formula:

[0080] Inhibition rate (%) = [A Blank ﹣(A 0 ﹣A)] / A Blank ×100%

[0081] I...

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