Microecologic preparation for inhibiting vibrio in litopenaeus vannamei culture as well as preparation and application

Abstract

The invention provides a microecologic preparation for inhibiting vibrio in litopenaeus vannamei culture as well as preparation and application, and relates to aquaculture. The microecologic preparation comprises at least one of bacillus safensis and bacillus pumilus, and at least one of lactobacillus plantarum and lactobacillus plantarum; when the microecologic preparation is in a form of microbial bacteria solution, the preparation method comprises the following step: mixing a bacillus safensis fermented solution, a bacillus pumilus fermented solution, a lactobacillus plantarum fermented solution and a lactobacillus plantarum fermented solution; at least one of vitamin C, fructo-oligosaccharide, mannitol, and the like is added as a protecting agent to the mixture. The microecologic preparation is applicable to the preparation of preparations for promoting the growing of litopenaeus vannamei, improving the immunity and disease resistance of the litopenaeus vannamei, preventing and controlling the diseases of the litopenaeus vannamei, increasing the survival rate, purifying culture water quality, and particularly preventing and controlling intestinal tract thickening, red-leg disease, eye-rot disease and white spot syndrome virus of the litopenaeus vannamei. The microecologic preparation is applicable to preparation of veterinary drugs, feed additives or water purifiers, and the like.

Description

technical field

[0001] The invention relates to aquaculture, in particular to a microecological preparation for inhibiting Vibrio in Litopenaeus vannamei culture, a preparation method and application thereof. Background technique

[0002] Since 2003, with the promotion of high-density farming mode, my country's shrimp farming industry has entered a period of rapid development. In 2006, the total amount of shrimp farming in my country was 1.24 million tons, accounting for 39.2% of the global farmed shrimp production, and it has remained the world's first for many years . However, the rapid development of intensive and high-density aquaculture has seriously damaged the ecological environment of aquaculture, and also produced a series of negative effects, including waste of feed resources, environmental deterioration, serious diseases, germplasm degradation, drug residues, etc. , especially disease problems are becoming more and more serious. Shrimp white spot virus, taura vir...

Examples

Embodiment 1

[0047] Embodiment 1 A kind of preparation of probiotics

[0048] The cultivation of the bacteria of each component in the probiotics:

[0049] Bacillus safensis (Bacillus safensis) 1A07588 deposit number CCTCC NO: M2013290 and Bacillus pumilus including Bacillus pumilus (Bacillus pumilus) 1A08151 deposit number CCTCC NO: M2013291 cultivation: the culture of starting strains Bacillus safensis and Bacillus pumilus Cultivate in the order of slant, liquid seed, and liquid fermentation, and the slant and seed medium are conventional nutrient agar medium or broth medium; fermentation medium formula (W / V): 3% bean cake powder, 1.5% starch, corn Syrup 1%, Glucose 0.5%, (NH 4 ) SO 4 0.1%, KH 2 PO 4 0.5%, NaH 2 PO 4 0.5%, MgSO 4 0.05%, MnSO 4 0.05%, the fermentation condition is 28~37℃, the aeration culture is 36-48h, the number of viable bacteria is ≥10 9 CFU / mL;

[0050] Lactobacillus plantarum includes Lactobacillus plantarum (Lactobacillus plantarum) 1A07806 preservati...

Embodiment 2

[0056] Application test 1 of embodiment 2 probiotics of the present invention in prawn culture

[0057] 1. Materials and methods

[0058] 1. Test product: the compound probiotic preparation bacterium liquid of embodiment 1, the total number of viable bacteria is more than 1 billion / ml.

[0059] 2. Test prawns: divide the prawns into 2 groups, 250 tails in each group, and set 3 repetitions in each group.

[0060] 3. Test water body: The test water is taken from sand-filtered seawater in Baicheng, Xiamen.

[0061] 4. Test site: The test is carried out in a half-ton glass fiber tank. The test cylinders were placed in an open place outdoors and placed randomly under natural conditions.

[0062]5. Test method: The test period is 45 days. The test adopts conventional prawn feed feeding, and the test group adds 1 kg of test product per ton of feed to ensure that the number of viable bacteria in each gram of feed is ≥ 10 6 CFU / mL, no test product was added to the control group. O...

Embodiment 3

[0072] The application test of the microecological preparation of the present invention in the culture of prawn shrimp fry of embodiment 3

[0073] 1. Test product: the compound probiotic preparation bacterium liquid of embodiment 1, the total number of live bacteria is more than 1 billion / ml.

[0074] 2. Experimental prawns: The prawns from seedlings to 30 days old are used throughout. Set up 3 experimental pools and 7 control pools.

[0075] 3. Test site: a prawn farm in Shentu Town, Zhangpu County.

[0076] 4, test method: when raising daily, get embodiment 1 compound bacterial agent pool and evenly splash. It is used once every three days, and the dosage per mu of aquaculture water body is 0.5-1L, and it is doubled when the water quality deteriorates. The control group did not use the bacteria agent. Get the composite bacterial agent of embodiment 1 at the same time and evenly spray on the feed surface after diluting with appropriate amount of water, mix and feed the p...

technical field

[0001] The invention relates to aquaculture, in particular to a microecological preparation for inhibiting Vibrio in Litopenaeus vannamei culture, a preparation method and application thereof. Background technique

[0002] Since 2003, with the promotion of high-density farming mode, my country's shrimp farming industry has entered a period of rapid development. In 2006, the total amount of shrimp farming in my country was 1.24 million tons, accounting for 39.2% of the global farmed shrimp production, and it has remained the world's first for many years . However, the rapid development of intensive and high-density aquaculture has seriously damaged the ecological environment of aquaculture, and also produced a series of negative effects, including waste of feed resources, environmental deterioration, serious diseases, germplasm degradation, drug residues, etc. , especially disease problems are becoming more and more serious. Shrimp white spot virus, taura vir...

Examples

Embodiment 1

[0047] Embodiment 1 A kind of preparation of probiotics

[0048] The cultivation of the bacteria of each component in the probiotics:

[0049] Bacillus safensis (Bacillus safensis) 1A07588 deposit number CCTCC NO: M2013290 and Bacillus pumilus including Bacillus pumilus (Bacillus pumilus) 1A08151 deposit number CCTCC NO: M2013291 cultivation: the culture of starting strains Bacillus safensis and Bacillus pumilus Cultivate in the order of slant, liquid seed, and liquid fermentation, and the slant and seed medium are conventional nutrient agar medium or broth medium; fermentation medium formula (W / V): 3% bean cake powder, 1.5% starch, corn Syrup 1%, Glucose 0.5%, (NH 4 ) SO 4 0.1%, KH 2 PO 4 0.5%, NaH 2 PO 4 0.5%, MgSO 4 0.05%, MnSO 4 0.05%, the fermentation condition is 28~37℃, the aeration culture is 36-48h, the number of viable bacteria is ≥10 9 CFU / mL;

[0050] Lactobacillus plantarum includes Lactobacillus plantarum (Lactobacillus plantarum) 1A07806 preservati...

Embodiment 2

[0056] Application test 1 of embodiment 2 probiotics of the present invention in prawn culture

[0057] 1. Materials and methods

[0058] 1. Test product: the compound probiotic preparation bacterium liquid of embodiment 1, the total number of viable bacteria is more than 1 billion / ml.

[0059] 2. Test prawns: divide the prawns into 2 groups, 250 tails in each group, and set 3 repetitions in each group.

[0060] 3. Test water body: The test water is taken from sand-filtered seawater in Baicheng, Xiamen.

[0061] 4. Test site: The test is carried out in a half-ton glass fiber tank. The test cylinders were placed in an open place outdoors and placed randomly under natural conditions.

[0062]5. Test method: The test period is 45 days. The test adopts conventional prawn feed feeding, and the test group adds 1 kg of test product per ton of feed to ensure that the number of viable bacteria in each gram of feed is ≥ 10 6 CFU / mL, no test product was added to the control group. O...

Embodiment 3

[0072] The application test of the microecological preparation of the present invention in the culture of prawn shrimp fry of embodiment 3

[0073] 1. Test product: the compound probiotic preparation bacterium liquid of embodiment 1, the total number of live bacteria is more than 1 billion / ml.

[0074] 2. Experimental prawns: The prawns from seedlings to 30 days old are used throughout. Set up 3 experimental pools and 7 control pools.

[0075] 3. Test site: a prawn farm in Shentu Town, Zhangpu County.

[0076] 4, test method: when raising daily, get embodiment 1 compound bacterial agent pool and evenly splash. It is used once every three days, and the dosage per mu of aquaculture water body is 0.5-1L, and it is doubled when the water quality deteriorates. The control group did not use the bacteria agent. Get the composite bacterial agent of embodiment 1 at the same time and evenly spray on the feed surface after diluting with appropriate amount of water, mix and feed the p...

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