A screening method for androgen chemical risk substances in toiletries

A technology for cleaning products and androgen, applied in scientific instruments, electric vehicle charging technology, climate sustainability, etc., can solve the problems of lagging supervision of chemical risk substances, insufficient prevention ability, and unreported detection methods, and achieve faster The effects of sustainable and healthy development, promotion of healthy and standardized development, and standardization of market economic rules and business order

CN107543891BActive Publication Date: 2020-01-03CHINESE ACAD OF INSPECTION & QUARANTINE
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Publication Date
2020-01-03

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Abstract

The invention discloses a method for screening androgen chemical risk substances in toiletries, comprising the following steps: (1) establishing an accurate mass database and a mass spectrum library of a compound to be tested; (2) pretreatment and detection of actual samples: After the pretreatment steps of extraction, centrifugation, purification, filtration and other pretreatment steps, the sample solution obtained is subjected to ultra-high pressure liquid chromatography-quadrupole-electrostatic field orbitrap high-resolution mass spectrometry analysis and detection; (3) Step (2) The obtained test results are compared and analyzed with the accurate mass database and mass spectral library established in step (1), to carry out the screening of androgen chemical risk substances. The screening method for the androgen chemical risk substances in the toiletries established by the invention has high accuracy, strong specificity and high sensitivity, and is applicable to daily detection and quality control of the toiletries.
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Description

technical field

[0001] The invention relates to a screening method for chemical substances, in particular to a screening method for androgen chemical risk substances in toiletries based on ultra-high pressure liquid chromatography-quadrupole-electrostatic field orbitrap high-resolution mass spectrometry check method. Background technique

[0002] 1. Chemical risk substances in toiletries have potential harmful effects on consumers' personal health.

[0003] From the very beginning of its birth, toiletries have provided protection for people's pursuit of a clean, healthy and fashionable life. With the continuous development and progress of society, people's pursuit of a clean, healthy and fashionable lifestyle has also been continuously upgraded, thus driving the development of toiletries. The industry has stepped into the fast lane of development and upgrading. At present, there are a wide variety of products in the cleaning and care products market, and functional, subdiv...

Examples

Embodiment 1

[0048] A method for detecting androgen chemical risk substances in toiletries, comprising the steps of:

[0049] After the pretreatment steps of extraction, centrifugation, purification, filtration and other pretreatment steps, the sample solution obtained by ultra-high pressure liquid chromatography-quadrupole-electrostatic field orbitrap high-resolution mass spectrometry analysis and detection; ultra-high pressure liquid chromatography-quadrupole In rod-electrostatic field orbitrap high-resolution mass spectrometry analysis and detection, the chromatographic separation conditions are as follows:

[0050] Chromatographic column: HSS T3 chromatographic column, length 100mm, inner diameter 2.1mm, particle size 1.8μm; column temperature: 25°C; flow rate: 0.35mL min -1 ;Injection volume: 5μL, see Table 1 for mobile phase and gradient elution program:

[0051] Table 1 Chromatographic mobile phase and gradient elution program

[0052]

[0053] In the ultra-high pressure liquid...

Embodiment 2

[0061] 1. Instruments and reagents

[0062] Dionex UltiMate 3000 fast high-performance liquid chromatography system (Thermo Fisher, USA); QExactive Focus quadrupole-electrostatic field orbitrap high-resolution mass spectrometer (Thermo Fisher, USA); Milli-Q ultrapure water instrument (Millipore, USA); Vortex-Genie 2 vortex oscillator (Scientific Industries, USA).

[0063] Methanol, acetonitrile (chromatographically pure, American Thermo Fisher Company); ammonium hydroxide, formic acid (chromatographically pure, American DimaTechnology Company); laboratory water is deionized water. Carbon eighteen, N-propyl ethylenediamine and graphitized carbon black (Germany Macherey-Nagel company); Multi-wall carbon nanotubes (Germany Miltenyi Biotec GmbH company); Anhydrous magnesium sulfate, sodium chloride (Guangzhou Xilong Chemical Industry limited liability company); 0.22 μm microporous membrane (Pall Corporation, USA).

[0064] Prepare 500-1000μg mL respectively -1 The standard stoc...