Preparation method and application of microbial agent for preventing and treating panax notoginseng root rot

Abstract

The invention relates to a preparation method and application of a microbial agent for preventing and treating panax notoginseng root rot, and belongs to the technical field of microbial pesticides. The microbial agent for preventing and treating the panax notoginseng root rot is prepared from a strain of streptomyces sp. YIM PH20246 with the preservation number of CCTCC (China Center for Type Culture Collection) NO: M 2018221 through a secondary fermentation method, and the preservation number of the streptomyces sp. YIM PH20246 is CCTCC NO: M 2018221. The microbial agent for preventing and treating the panax notoginseng root rot has good in-vitro inhibition activity on panax notoginseng root rot protofungi fusarium oxysporum, fusarium solani and cucumber wilt germs, has the advantages of being easy to produce repeatedly, economical, safe and environmentally friendly, and has potential and practical application value in biological prevention and treatment of the panax notoginseng root rot.

Description

technical field

[0001] The invention relates to a preparation method and application of a microbial control agent for notoginseng root rot, belonging to the technical field of microbial pesticides. Background technique

[0002] Root rot of Panax notoginseng is a typical soil-borne disease. Soil bacteria is an important factor that causes root rot of Panax notoginseng. There are three types of pathogens that can cause root rot: fungi, bacteria, and nematodes. Pathogens can cause disease alone or in combination Among them, fungi such as Fusarium solani, Fusarium oxysporum and Phoma phloem are the main pathogenic bacteria of field root rot of Panax notoginseng. At present, the prevention and treatment of Panax notoginseng root rot still largely depends on the use of chemical pesticides such as organochlorine, organophosphorus, and carbamate for root irrigation. Due to the extensive use of pesticides, the residues of Panax notoginseng pesticides have seriously Affected the qual...

Examples

Embodiment 1

[0019] Embodiment one: the preparation method of microbial control Radix Notoginseng root rot agent

[0020] The bacterial strain used in the present invention is Streptomyces sp.YIM PH20246 (Streptomyces sp.YIM PH20246), which was deposited in China Typical Culture Collection Center on April 19, 2018, address: Wuhan University, Wuhan, China, and the preservation number is CCTCC NO : M 2018221.

[0021] The preparation method of the microbial control Radix Notoginseng root rot agent of the present invention comprises the following steps:

[0022] (1) Test tube seed culture: inoculate the Streptomyces sp. YIM PH20246 (Streptomyces sp. YIM PH20246) strain with the preservation number CCTCC NO: M2018221 on the solid medium slant of the test tube, and cultivate it at 28°C for 6 days to obtain the strain test tube kind.

[0023] Among them, the solid medium formula is: yeast powder 4.0g; glucose 4.0g; malt extract 5.0g; multivitamin 3.5mg; agar powder 15.0g; water 1000mL; pH 7.2....

Embodiment 2

[0028] Embodiment 2: Microbiological control of Panax notoginseng root rot agent inhibits the test of pathogenic fungi of Panax notoginseng root rot

[0029] Indoor toxicity test (inhibition of spore germination method and plate antibacterial test method) test was used to measure the inhibitory effect of the microbial control agent for controlling root rot of Panax notoginseng prepared by the above method on the pathogenic fungus of root rot of Panax notoginseng.

[0030] A. Inhibition of spore germination test

[0031] 1. Microbial anti-ginseng root rot agent for preparation test

[0032] The specific preparation method of the agent for preventing and controlling root rot of Panax notoginseng is the same as that described in the summary of the invention.

[0033] 2. Preparation of Panax notoginseng root rot pathogenic fungus spore suspension: Inoculate Panax notoginseng root rot pathogenic fungi Fusarium oxysporum, Fusarium solani, Pointomyces graminopsis and Cucumber wilt f...

Embodiment 3

[0048] Embodiment three: the preparation method of microbial control Radix Notoginseng root rot agent

[0049] The preparation method of the microbial control Radix Notoginseng root rot agent of the present invention comprises the following steps:

[0050] (1) Test tube seed culture: inoculate the Streptomyces sp. YIM PH20246 (Streptomyces sp. YIM PH20246) strain with the preservation number CCTCC NO: M2018221 on the solid medium slant of the test tube, and cultivate it at 28°C for 6 days to obtain the strain test tube kind.

[0051] Among them, the solid medium formula is: yeast powder 4.0g; glucose 4.0g; malt extract 5.0g; multivitamin 3.5mg; agar powder 15.0g; water 1000mL; pH 7.2.

[0052] (2) Cultivate liquid seeds: Use 500mL Erlenmeyer flasks for culture, first prepare liquid seed medium, fill each Erlenmeyer flask with 100mL liquid medium, sterilize at 120°C for 30 minutes, and insert after cooling 1cm 2 Large strains were cultured in test tubes at a temperature of 2...

technical field

[0001] The invention relates to a preparation method and application of a microbial control agent for notoginseng root rot, belonging to the technical field of microbial pesticides. Background technique

[0002] Root rot of Panax notoginseng is a typical soil-borne disease. Soil bacteria is an important factor that causes root rot of Panax notoginseng. There are three types of pathogens that can cause root rot: fungi, bacteria, and nematodes. Pathogens can cause disease alone or in combination Among them, fungi such as Fusarium solani, Fusarium oxysporum and Phoma phloem are the main pathogenic bacteria of field root rot of Panax notoginseng. At present, the prevention and treatment of Panax notoginseng root rot still largely depends on the use of chemical pesticides such as organochlorine, organophosphorus, and carbamate for root irrigation. Due to the extensive use of pesticides, the residues of Panax notoginseng pesticides have seriously Affected the qual...

Examples

Embodiment 1

[0019] Embodiment one: the preparation method of microbial control Radix Notoginseng root rot agent

[0020] The bacterial strain used in the present invention is Streptomyces sp.YIM PH20246 (Streptomyces sp.YIM PH20246), which was deposited in China Typical Culture Collection Center on April 19, 2018, address: Wuhan University, Wuhan, China, and the preservation number is CCTCC NO : M 2018221.

[0021] The preparation method of the microbial control Radix Notoginseng root rot agent of the present invention comprises the following steps:

[0022] (1) Test tube seed culture: inoculate the Streptomyces sp. YIM PH20246 (Streptomyces sp. YIM PH20246) strain with the preservation number CCTCC NO: M2018221 on the solid medium slant of the test tube, and cultivate it at 28°C for 6 days to obtain the strain test tube kind.

[0023] Among them, the solid medium formula is: yeast powder 4.0g; glucose 4.0g; malt extract 5.0g; multivitamin 3.5mg; agar powder 15.0g; water 1000mL; pH 7.2....

Embodiment 2

[0028] Embodiment 2: Microbiological control of Panax notoginseng root rot agent inhibits the test of pathogenic fungi of Panax notoginseng root rot

[0029] Indoor toxicity test (inhibition of spore germination method and plate antibacterial test method) test was used to measure the inhibitory effect of the microbial control agent for controlling root rot of Panax notoginseng prepared by the above method on the pathogenic fungus of root rot of Panax notoginseng.

[0030] A. Inhibition of spore germination test

[0031] 1. Microbial anti-ginseng root rot agent for preparation test

[0032] The specific preparation method of the agent for preventing and controlling root rot of Panax notoginseng is the same as that described in the summary of the invention.

[0033] 2. Preparation of Panax notoginseng root rot pathogenic fungus spore suspension: Inoculate Panax notoginseng root rot pathogenic fungi Fusarium oxysporum, Fusarium solani, Pointomyces graminopsis and Cucumber wilt f...

Embodiment 3

[0048] Embodiment three: the preparation method of microbial control Radix Notoginseng root rot agent

[0049] The preparation method of the microbial control Radix Notoginseng root rot agent of the present invention comprises the following steps:

[0050] (1) Test tube seed culture: inoculate the Streptomyces sp. YIM PH20246 (Streptomyces sp. YIM PH20246) strain with the preservation number CCTCC NO: M2018221 on the solid medium slant of the test tube, and cultivate it at 28°C for 6 days to obtain the strain test tube kind.

[0051] Among them, the solid medium formula is: yeast powder 4.0g; glucose 4.0g; malt extract 5.0g; multivitamin 3.5mg; agar powder 15.0g; water 1000mL; pH 7.2.

[0052] (2) Cultivate liquid seeds: Use 500mL Erlenmeyer flasks for culture, first prepare liquid seed medium, fill each Erlenmeyer flask with 100mL liquid medium, sterilize at 120°C for 30 minutes, and insert after cooling 1cm 2 Large strains were cultured in test tubes at a temperature of 2...

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