Extract of mulberry twig and its extracting process and novel usage

Abstract

An extract of mulberry twigs is dislcosed, which can be used to prepare the food or the medicine to cure hyperuricemia and gout diseases. The said extract is prepared from mulberry twigs through extracting in solvent (aqueous solution of alcohol, chloroform, acetone, etc) at 10-100 deg.C more than one time, mergint the liquid extracts, recovering solvent, regulating pH value to 7.5-9.5, centrifugal separation to obtain supernatant, regulating pH value to 4-6, deposition, water washing wand drying. Several other approaches for purifying it are also disclosed.

Description

Technical field:

[0001] The invention relates to a natural plant extract and its extraction process and new application, in particular to preparing the mulberry branch extract into a health food for preventing hyperuricemia and gout and a medicine for treating hyperuricemia and gout. Background technique:

[0002] Gout is a group of chronic life-long diseases characterized by purine metabolic disorders. Its pathogenesis is complex, and its clinical features are hyperuricemia and the resulting gouty arthritis recurrent attacks, tophi deposition, gouty chronic arthritis and joint deformities. Often involves the kidneys causing chronic interstitial nephritis and the formation of uric acid nephrolithiasis.

[0003] Hyperuricemia refers to a state in which uric acid in the blood exceeds normal levels. It is a prelude to gout and an important clinical manifestation of gout. Gouty arthritis is caused by excessive urate deposition in the joint tissue, which stimulates the joint tis...

Examples

Embodiment 1

[0026] Example 1: Effect of Mori Mori Extract WE on hyperuricemia and normal mice and rats serum uric acid levels and gouty arthritis caused by sodium urate in rats

[0027] Take dried mulberry branch decoction pieces, decoct three times with 10 times, 8 times, and 6 times of water, each time for 1 hour. The decoction liquids were combined, concentrated under reduced pressure, and dried to obtain WE with a yield of 11.52% and a total flavonoid content of 1.1%.

[0028] The results of the effect of WE on uric acid levels in hyperuricemia and normal mice and rats are shown in Table 1 and Table 2, respectively. Under these experimental conditions, the high, medium and low dose groups of WE all significantly reduced the serum uric acid levels of hyperuricemia mice and rats, among which the high and medium dose groups of WE could reduce the serum uric acid levels of hyperuricemia mice. Return to normal; the middle, low and high dose groups of WE can restore the serum uric acid lev...

Embodiment 2

[0038] Example 2: Effects of Mori Mori Extract AE-PH on serum uric acid levels in hyperuricemia and normal mice

[0039] Take the dried mulberry branch decoction pieces, and extract with 8 times, 8 times, and 6 times the amount of 80% ethanol aqueous solution under heat reflux three times, each time for 2 hours. Combine the extracts, recover ethanol under reduced pressure, adjust the pH to 8.5 with 10% NaOH, centrifuge after fully standing, adjust the supernatant to pH 4-6 with hydrochloric acid, let stand, take the precipitate, wash with distilled water until neutral, AE-PH is obtained after drying with a yield of 0.5% and a total flavonoid content of 23%.

[0040] AE-PH can significantly reduce the serum uric acid level of mice with hyperuricemia, which has a good dose-effect relationship (Table 4), but has no significant effect on the serum uric acid level of normal mice (data omitted).

[0041] group

[0042] Compared with the model blank group: P** P*** P<0.001...

Embodiment 3

[0043] Example 3: Effects of Morus mulberry extracts AE1, AE1-PE and AE1-1~AE1-4 on serum uric acid levels in hyperuricemia and normal mice

[0044] Take the dried mulberry branch decoction pieces, and extract with 8 times, 8 times, and 6 times the amount of 80% ethanol aqueous solution under heat reflux three times, each time for 2 hours. The extracts were combined, concentrated under reduced pressure, and dried to obtain AE1 with a yield of 4.18% and a total flavonoid content of 2.0%.

[0045] After mixing AE1 with diatomaceous earth, the Soxhlet reflux method was used to degrease petroleum ether and dry to obtain AE1-PE with a yield of 3.2% and a total flavonoid content of 3.5%.

[0046] After mixing AE1 with diatomaceous earth, Soxhlet reflux method, degreasing with petroleum ether, and sequentially extracting with chloroform, ethyl acetate, methanol and water until colorless, each extract was concentrated and dried to obtain AE1-1, The yields of AE1-2, AE1-3 and AE1-4 we...

Technical field:

[0001] The invention relates to a natural plant extract and its extraction process and new application, in particular to preparing the mulberry branch extract into a health food for preventing hyperuricemia and gout and a medicine for treating hyperuricemia and gout. Background technique:

[0002] Gout is a group of chronic life-long diseases characterized by purine metabolic disorders. Its pathogenesis is complex, and its clinical features are hyperuricemia and the resulting gouty arthritis recurrent attacks, tophi deposition, gouty chronic arthritis and joint deformities. Often involves the kidneys causing chronic interstitial nephritis and the formation of uric acid nephrolithiasis.

[0003] Hyperuricemia refers to a state in which uric acid in the blood exceeds normal levels. It is a prelude to gout and an important clinical manifestation of gout. Gouty arthritis is caused by excessive urate deposition in the joint tissue, which stimulates the joint tis...

Examples

Embodiment 1

[0026] Example 1: Effect of Mori Mori Extract WE on hyperuricemia and normal mice and rats serum uric acid levels and gouty arthritis caused by sodium urate in rats

[0027] Take dried mulberry branch decoction pieces, decoct three times with 10 times, 8 times, and 6 times of water, each time for 1 hour. The decoction liquids were combined, concentrated under reduced pressure, and dried to obtain WE with a yield of 11.52% and a total flavonoid content of 1.1%.

[0028] The results of the effect of WE on uric acid levels in hyperuricemia and normal mice and rats are shown in Table 1 and Table 2, respectively. Under these experimental conditions, the high, medium and low dose groups of WE all significantly reduced the serum uric acid levels of hyperuricemia mice and rats, among which the high and medium dose groups of WE could reduce the serum uric acid levels of hyperuricemia mice. Return to normal; the middle, low and high dose groups of WE can restore the serum uric acid lev...

Embodiment 2

[0038] Example 2: Effects of Mori Mori Extract AE-PH on serum uric acid levels in hyperuricemia and normal mice

[0039] Take the dried mulberry branch decoction pieces, and extract with 8 times, 8 times, and 6 times the amount of 80% ethanol aqueous solution under heat reflux three times, each time for 2 hours. Combine the extracts, recover ethanol under reduced pressure, adjust the pH to 8.5 with 10% NaOH, centrifuge after fully standing, adjust the supernatant to pH 4-6 with hydrochloric acid, let stand, take the precipitate, wash with distilled water until neutral, AE-PH is obtained after drying with a yield of 0.5% and a total flavonoid content of 23%.

[0040] AE-PH can significantly reduce the serum uric acid level of mice with hyperuricemia, which has a good dose-effect relationship (Table 4), but has no significant effect on the serum uric acid level of normal mice (data omitted).

[0041] group

[0042] Compared with the model blank group: P** P*** P<0.001...

Embodiment 3

[0043] Example 3: Effects of Morus mulberry extracts AE1, AE1-PE and AE1-1~AE1-4 on serum uric acid levels in hyperuricemia and normal mice

[0044] Take the dried mulberry branch decoction pieces, and extract with 8 times, 8 times, and 6 times the amount of 80% ethanol aqueous solution under heat reflux three times, each time for 2 hours. The extracts were combined, concentrated under reduced pressure, and dried to obtain AE1 with a yield of 4.18% and a total flavonoid content of 2.0%.

[0045] After mixing AE1 with diatomaceous earth, the Soxhlet reflux method was used to degrease petroleum ether and dry to obtain AE1-PE with a yield of 3.2% and a total flavonoid content of 3.5%.

[0046] After mixing AE1 with diatomaceous earth, Soxhlet reflux method, degreasing with petroleum ether, and sequentially extracting with chloroform, ethyl acetate, methanol and water until colorless, each extract was concentrated and dried to obtain AE1-1, The yields of AE1-2, AE1-3 and AE1-4 we...