31 results about "Sensitivity drugs" patented technology

A method for establishing a three-dimensional skin model of the present invention comprises the following steps: 1) preparing an extracellular matrix polymer hydrogel solution; 2) mixing fibroblasts with the extracellular matrix polymer hydrogel solution, and putting them into a culture chamber Cultivate; 3) Inoculate keratinocytes and culture through air-liquid interface to obtain a three-dimensional skin model including dermis, epidermis and stratum corneum. The method of the invention can effectively establish a three-dimensional skin model, and the preparation method is simple and convenient, can be used for screening skin anti-inflammatory and anti-sensitivity drugs, and has excellent application prospects.

Embodiments of the invention provide a manufacturing method for 3D-printed artificial bone, belonging to the field of medical technology. The manufacturing method comprises the following steps: acquiring a first sensitive medicine; acquiring a second sensitive medicine; dissolving chitosan with weight percentage of 1 to 90 wt% in double-distilled water to obtain a solvent; dissolving the first sensitive medicine in the solvent and preparing a first suspension; adding a calcium-containing compound with weight percentage of 5 to 50% into the first suspension to prepare a first solid; dissolvingthe second sensitive medicine in the solvent and preparing a second suspension; adding the first solid into the second suspension, and then adding a calcium-containing compound with weight percentageof 0.5 to 30% to prepare a first paste material; and adding the first paste material into a biological 3D printer for printing so as to form the first artificial bone. In a medicine-containing paste material for the artificial bone, the medicine-containing solid is prepared from chitosan and double-distilled water, so a plurality of medicines are released at the same time; and drug effect is improved due to combined usage of the medicines.

The invention provides a manufacturing method of a 3D printed artificial bone, and relates to the technical field of medicine. The manufacturing method comprises the following steps: obtaining a firstsensitive drug; obtaining a second sensitive drug, wherein the sensitivity of the first sensitive drug is lower than that of the second sensitive drug; dissolving 0.1 to 20 wt% of copolymer containing ethyl acetate into 1,4-dioxane to form a solvent; dissolving the first sensitive drug in the solvent to form a first turbid liquid; adding 5 to 50 wt% of calcium containing compounds into the firstturbid liquid to prepare a first solid; dissolving the second sensitive drug in the solvent to form a second turbid liquid; adding the first solid into the second turbid liquid, then adding 0.5 to 30wt% of calcium containing compounds to prepare a first paste like material; adding the first paste like material into a biological 3D printing machine, and carrying out printing to form a first artificial bone. The technical effects of step-by-step drug administration and prolonged sustained release time are realized.

The invention belongs to the field of gene engineering, discloses an application of human miR-148a to preparation of medicine for improving adipose cell insulin sensitivity, and provides an application of human miR-148a to preparation of medicine for improving adipose tissue insulin sensitivity and / or promoting glucose uptake of adipose tissue under a state of insulin stimulation in the technical scheme. Researches show that overexpression of the human miR-148a in adipose cells can obviously promote glucose uptake of the adipose cells under the state of insulin stimulation, and the human miR-148a can be used for preparing the medicine for improving adipose tissue insulin sensitivity and / or promoting glucose uptake of the adipose tissue under the state of insulin stimulation as well as used for preparing medicine for preparing diabetes mellitus type 2.