2results about How to "Easy to clear" patented technology

This utility model discloses a car wash pool that is easy to clean, belonging to the field of car wash pool cleaning technology. It includes a car wash pool with multiple equally spaced grooves at the bottom and a frame installed on the top. The frame is equipped with cleaning components for treating mud and sand in the grooves. A collection pool is installed on the outer wall of the car wash pool, and the collection pool and the car wash pool are connected by a rectangular hole. A lifting frame is installed inside the collection pool, and the lifting frame is equipped with a lifting and cleaning component. Therefore, by setting trapezoidal sand-pushing blocks adapted to the shape of the trapezoidal grooves, with each sand-pushing block corresponding to a groove, the sand-pushing blocks can closely fit the inner wall of the grooves during movement, thoroughly scraping away and pushing the mud and sand attached to the bottom and side walls of the grooves into the collection pool, effectively solving the problems of mud and sand accumulation and incomplete cleaning in traditional trenches.

This invention relates to a method for constructing a polycystic kidney disease (PCD) model and its applications. The method involves overexpressing the MYCN gene in a target animal to obtain a PCD model that leads to PCD-related phenotypes. The method includes the following steps: obtaining a first strain of mice with Rosa26 knock-in overexpressing the CAG-LSL-HA tag-MYCN-IRES-BFP-Wpre-polyA gene; obtaining a second strain of mice by inserting Cre-WPRE-polyA into the start codon of the Pax8 gene; and crossing the first and second strains of mice to obtain a MYCN-overexpressing PCD model. This invention employs various experimental methods for validation, including histopathological analysis, immunohistochemical staining, and Western blotting. The model provided by this invention overcomes the limitations of existing in vitro cell models, organoids, and existing animal models in terms of limited phenotypes. The established MYCN-overexpressing PCD animal model exhibits stable disease progression and a short disease cycle, which not only helps to elucidate the disease mechanism but also serves as an ideal platform for drug screening and efficacy evaluation.