Technique for producing rice bran hemicellulose B by using rice bran pilotscale experiment
A technology of rice bran hemicellulose and process method, which is applied in the technical field of producing rice bran hemicellulose B on a pilot scale with rice bran, and can solve the problems of difficult industrial production, complicated process, and high production cost
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Embodiment 1
[0014] Dissolve 100 kg of undefatted fresh rice bran in 2000 L of 0.6% high-temperature resistant glucoamylase-containing ethyl acetate buffer, the pH of the buffer is 4.8, and the temperature is kept at 60° C. for 24 hours. After centrifugation and washing, add 2%-4% NaOH solution to the precipitate and keep it for 24 hours. After centrifugation, neutralize the extract with acetic acid to make the pH of the solution around 5.0. After centrifugation to remove the residue, use 4 times the volume of 95 % Ethanol precipitation, finally drying the precipitate at 60°C for 14 hours, and finely grinding to obtain about 0.3 kg of RBHB preparation.
Embodiment 2
[0016] Dissolve 50 kilograms of undefatted fresh rice bran in 1000L of 0.6% high temperature resistant glucoamylase-added ethyl sodium acetate buffer. The pH of the buffer is 4.8, kept at 60°C for 36 hours, centrifuged, washed, and precipitated Add 2%-4% NaOH solution and keep for 30 hours. After the solution is centrifuged, the extract is neutralized with acetic acid to make the solution pH 5.0. After the residue is removed by centrifugation, the solution is precipitated with 4 times volume of 95% ethanol, and finally the precipitate After drying at 60°C for 18 hours, about 3.5 kg of RBHB preparation was obtained after fine grinding.
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