Total bilirubin determination reagent kit

Abstract

The present invention discloses a total bilirubin determining reagent box, which is liquid double reagent formed by reagent 1 and reagent 2. The constituents and the consistence range of the reagent 1 are: disodium of 13.5-81g / L, sodium dihydrogenphosphate of 0.6-3.6g / L, sodium chloride of 9.0g / L, ethylenediaminetetraacetic acid disodium salt of 0.2-1g / L, tween of -80 0.5-2ml / L, and sodium dodecyl sulfate of 1-10g / L. The constituents and the consistence range of the reagent 2 are: disodium of 1g / L, sodium chloride of 9g / L, sodium persulfate of 11.9-23.8g / L, and sulphuric acid of 0.1-2ml / L. The total bilirubin determining reagent box is stable and environment-friendly, and has proper linearity range, high test accuracy, high precision, high sensitivity and strong anti-jamming performance.

Description

technical field [0001] The invention relates to a kit for measuring total bilirubin in serum, plasma or urine, in particular to a kit for measuring total bilirubin by a sodium persulfate oxidation method. Background technique [0002] Total bilirubin is the general term for conjugated and unconjugated bilirubin produced by heme metabolism in the human body. Abnormally high total bilirubin is often seen in liver and gallbladder diseases. In recent years, some studies have shown that bilirubin in blood is lower than normal Often associated with coronary heart disease. Therefore, the determination of total bilirubin in human serum, plasma or urine is one of the most routine items in medical testing. [0003] The present inventor once proposed a kit for determining total bilirubin by chemical oxidation of potassium persulfate, patent application number 200610154480.2. This patent application provides a kit for the determination of total bilirubin with the existing technology o...

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Problems solved by technology

However, hemoglobin has obvious absorption peaks at wavelengths around 450nm (main wavelength) and 546nm (secondary wavelength), which seriously interferes with the determination of total bilirubin.

[0006] 2. Existing kits are inaccurate when detecting samples with severe lipid turbidity

At present, people's diet is high in fat content, and the blood is not necessarily fasted overnight, so the probability of fat turbidity in serum samples is also increasing, resulting in a high sample blank and affecting the determination of bilirubin

The surfactant Triton X-100 (Triton X-100) used in the existing kits mainly solves the solubility problem of the protein in the sample, and also has a certain anti-interference effect on slight fat turbidity, but if the sample is seriously fat turbid, it will affect Bilirubin determination

Although increasing the amount of Triton 100 can promote the disappearance of fat turbidity, it takes a long time to balance after adding reagent 2, which leads to fluctuations in the response curve, and the irregular response curve makes the measured value inaccurate

[0007] 3. Potassium persulfate in the existing kit slowly degrades to generate acidic substances, which causes the pH value of the reagent to drop after a certain period of time, and the reaction system does not meet the required pH value environment during measurement, which will affect the measurement accuracy

In addition, the content of potassium persulfate is too high. Although the upper limit of detection linearity is large, it is easy to cause potassium persulfate to pollute the post-test items when measuring samples in an automatic biochemical analyzer, resulting in inaccurate measurement.

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