47results about How to "Specific response" patented technology

The present invention is directed to an artificial nucleic acid, particularly to an artificial RNA suitable for use in treatment and / or prophylaxis of an infection with Respiratory syncytial virus (RSV) or a disorder related to such an infection. The invention further concerns a method of treating or preventing a disorder or a disease, first and second medical uses of the artificial RNA, compositions, and vaccines. Further, the invention is directed to a kit, particularly to a kit of parts, comprising the artificial RNA, compositions and vaccines.

The present invention discloses a total bilirubin determining reagent box, which is liquid double reagent formed by reagent 1 and reagent 2. The constituents and the consistence range of the reagent 1 are: disodium of 13.5-81g / L, sodium dihydrogenphosphate of 0.6-3.6g / L, sodium chloride of 9.0g / L, ethylenediaminetetraacetic acid disodium salt of 0.2-1g / L, tween of -80 0.5-2ml / L, and sodium dodecyl sulfate of 1-10g / L. The constituents and the consistence range of the reagent 2 are: disodium of 1g / L, sodium chloride of 9g / L, sodium persulfate of 11.9-23.8g / L, and sulphuric acid of 0.1-2ml / L. The total bilirubin determining reagent box is stable and environment-friendly, and has proper linearity range, high test accuracy, high precision, high sensitivity and strong anti-jamming performance.

The invention provides a preparation method of 2-methyl-4-acetoxy-2-butenal (I) with high yield. The preparation method comprises the steps: with 2,5-dihydrofuran (II) and synthesis gas as raw materials, carrying out hydroformylation reaction under the action of a catalyst to prepare 3-formyl tetrahydrofuran (III); then carrying out a reaction with an acetylation reagent under the action of a catalyst to prepare 2-formyl-4-acetoxy-1-butene (IV); and carrying out double bond isomerization under the action of a catalyst to obtain 2-methyl-4-acetoxy-2-butenal (I). The method has the advantages ofcheap and accessible raw materials and low cost; the process flow is short, the reaction is easy to realize, the operation is safe, simple and convenient, the wastewater yield is low, and the green and environment-friendly effects are achieved; the method has the advantages of stable reaction intermediate product, proper reaction activity, high reaction selectivity, fewer side reactions and highyield, and is suitable for industrial production.

The invention relates to a method of rapidly preparing a rhodamine dye with a plurality of active functional groups under the mild condition and belongs to the technical field of fine chemicals. The method is used for synthesizing rhodamine with specific functional groups by carrying out simple chemical modification on raw materials and has an important guiding significance to derivatization of the rhodamine dye. Compared with the commercially available rhodamine dye, the rhodamine dye has multiple active functional groups and is beneficial to derivatization of the rhodamine dye. By adopting the method, the rhodamine dye with the specific functional groups can be synthesized by 6-7h only without protection of inert gas, so that the synthesis time of the rhodamine dye is greatly saved, the purpose of the rhodamine dye is expanded, the synthesis yield of the rhodamine dye is greatly increased, and the separation difficulty of the rhodamine dye is prevented. Through the advantages, the purpose of the rhodamine dye is expanded, so that the rhodamine dye can be fully developed in the fields of biolabeling, cell imaging, fluorescent probes and the like.

The invention relates to the field of chemical synthesis, particularly to a novel synthetic process of an antibiotic material drug special for animals, in particular to a novel synthetic method of cefquinome sulfate. The method comprises the following steps of: taking GCLE (7-Phenylacetamide-3-chlorormethyl-3-cepham-4-carboxylic acid p-meth-oxybenzyl ester) as a starting material, and reacting with 5,6,7,8-tetrahydroquinoline after chlorine is replaced by iodine in C-3 chlorine methyl; by preparing an intermediate (7), successively stripping C-7 amino protection and C-4 carboxyl protection under the action of phosphorus pentachloride and phenol; reacting with AE-active ester in the existing of triethylamine; and preparing cefquinome sulfate. The method effectively solves the problems of stringent requirements of reaction condition of an original process route, complicated reaction system, difficult separation and purification, low purity and bad tincture of products and the like. The method has the advantages of simple technology, mild reaction condition, high yield, low cost and high quality and is suitable for industrialized production; and raw auxiliary materials have low priceand are easily obtained.

The invention provides a preparation method of 2-chloro-5-nitropyridine. The preparation method comprises the following steps: preparing 2-hydroxy-5-nitropyridine by taking 2-nitroacetaldehyde diethylacetal as an initial raw material through two methods; and then carrying out a chlorination reaction on the 2-hydroxy-5-nitropyridine and a chlorination reagent to prepare the 2-chloro-5-nitropyridine. The method has the advantages of cheap and accessible raw materials and low cost, does not use a diazotization hydrolysis reaction, is safe, simple and convenient to operate, does not use mixed acid, is less in wastewater yield and environmentally-friendly, does not use a nitration reaction, is high in reaction selectivity, few in side reactions, simple in post-treatment and high in product yield and product, and is suitable for industrial production.

The invention provides a preparation method of kresoxim-methyl, which comprises the following steps: carrying out an etherification reaction on 2-halogenated methyl halogenated benzene and 2-methylphenol to prepare 2 -(2-methylphenoxy methyl) halogenated benzene, and carrying out a Grignard reaction on the 2-(2-methylphenoxy methyl) halogenated benzene and magnesium metal to prepare reaction liquid containing a Grignard reagent; dropwise adding the obtained reaction liquid containing the Grignard reagent into dimethyl oxalate to prepare 2-(2-methyl phenoxy methyl) phenyl methyl oxalate, and finally performing condensation reaction with methoxylamine salt to prepare kresoxim-methyl. The method has the advantages of cheap and accessible raw materials and low cost; the method has the advantages of short process flow, easy realization of reaction conditions, safe, simple and convenient operation, less process wastewater generation amount, greenness and environmental protection, and can prepare kresoxim-methyl only through three steps of reactions; raw materials and intermediate products are high in stability, high in reaction activity and selectivity and less in side reaction; and theobtained kresoxim-methyl has few impurities and high purity and yield, and is beneficial to industrial production of kresoxim-methyl.

The invention relates to a preparation method for a cephalosporin anti-infective drug-cefazedone sodium, belonging to the field of pharmaceutical synthesis. According to the invention, the method uses GCLE as a raw material to substitute 7-ACA and overcomes the defects of low yield, high pollution and the like in prior art; the preparation method with mild reaction conditions, little side reaction and simple process is provided; meanwhile, the method has the advantages of cheap and easily-available raw materials, low cost, high product yield, high product purity and applicability to industrial production.

The invention discloses a method for preparing cholesterol by a microbial conversion method. The method is characterized in that cholesterol is prepared from raw material lanolin completely by microbial conversion through the steps of: 1) crushing raw material and adding a carbon source and a nitrogen source to prepare a seed medium and a fermentation medium; 2) screening strains; 3) inoculating the screened strain and then stirring and fermenting; and 4) separating, purifying and crystallizing. The method has the characteristics of mild conditions, specific reaction, small pollution and the like in preparing cholesterol. The cholesterol prepared by the method has high purity and yield, and conforms to the concept of environment protection.

The invention belongs to the technical field of tobacco analysis and detection and discloses a pretreatment method for analyzing the content of chrome in cigarette paper by HPLC-ICP / MS. The cigarette paper sample is subjected to shaking extraction by using a NaOH solution, the extraction solution is filtered by an aqueous filter membrane to obtain an initial filter solution, the pH value of the initial filter solution is adjusted by using an HCl solution, and finally the obtained filter solution is used as the analysis solution of the hexavalent chrome; or after the pH value of the initial filter solution is adjusted, the adjusted filter solution is allowed to complex with EDTA solution, and finally the complex reaction solution is used as the analysis solution of the trivalent chrome and the hexavalent chrome in the cigarette paper by HPLC-ICP / MS. The invention provides the chromatographic separation pretreatment method for the separation and measurement process of the trivalent chrome and the hexavalent chrome in the cigarette paper by using the high performance lquid chromatography (HPLC)-inductively coupled plasma mass spectrometry(ICP / MS). The pretreatment method has the advantages of convenience in operation, specific reaction and easiness in control, and provides powerful technical basis for measurement of the trivalent chrome and the hexavalent chrome in bulk samples.

The invention discloses a synthesis method of a pyridine derivative 2-tert-butoxy-6-methylene chloropyridine. The synthesis method of the pyridine derivative 2-tert-butoxy-6-methylene chloropyridine comprises the following steps: carrying out reaction on low-cost 2-bromo-6-tert-butoxy pyridine taken as a raw material as well as n-butyl lithium and DMF (dimethyl formamide) sequentially in presence of N2 and at low temperature, thus preparing a pyridine aldehyde compound; reducing aldehyde to obtain alcohol in a corresponding structure by utilizing sodium borohydride or lithium aluminium hydride as a reducing agent; and replacing hydroxyl in alcohol by utilizing a chlorine atom, thus obtaining a pyridine derivative containing methylene chloride and tert-butoxy groups with relatively high activity. The synthesis method of the pyridine derivative 2-tert-butoxy-6-methylene chloropyridine has the advantages that overall reaction steps are less, the reaction time is short, and the yield is high; and the prepared 2-tert-butoxy-6-methylene chloropyridine is easy to coordinate with a metal atom or other atoms and can be taken as a pyridine derivative ligand to be coordinated with metal, so as to prepare an organic metal catalyst or be applied to synthesis of a pyridine derivative drug.

The invention provides a preparation method of 5,6-dihydropyridine-2(1H)-one derivatives, specifically 1-(piperidin-2-one-1-yl)-4-(5,6-di The preparation method of hydrogen-3-R substituent pyridine-2(1H)-one-1-yl)benzene, the R substituent is chlorine or morpholin-4-yl. The present invention uses p-acetamidoaniline as a raw material, undergoes amidation with δ-valerolactone, halogenates with a halogenating agent or sulfonylates with sulfonyl chloride, condenses, deacetylates, and then reacts with 2,2-dichloro-δ - Amidation of valerolactone, halogenation with halogenating reagents or sulfonylation with sulfonyl chloride, followed by condensation elimination or condensation elimination substitution in the presence of morpholine to prepare the target product. The raw materials used in the preparation method of the invention are cheap and easy to obtain, and the cost is low; the process is simple and easy to operate, the reaction conditions are easy to realize, the amount of waste water generated is small, and it is safe and green; the reaction selectivity of each step is high, the product yield and purity are high, and it is beneficial to industrial production.

The invention belongs to the field of biological detection, in particular relates to a method for detecting enzymatic activity by using quantum dot fluorescence. The method provided by the invention is used for detecting the enzymatic activity through measuring the influence of a reaction system to water-soluble quantum dot fluorescence property in the enzyme reaction process. The method concretely comprises the following steps of: adding a certain quantity of enzymes to be detected in a reaction system containing water-soluble quantum dots or further in a reaction system containing coenzymes, enabling the fluorescence intensity of the water-soluble quantum dots to be varied due to the reaction of the enzymes to be detected, and realizing the high selective quantitative detection on the activity of the enzymes to be detected through the variable quantity of the fluorescence intensity of the water-soluble quantum dots. The method provided by the invention is simple in operation, rapid to detect and low in cost and can be used for detecting the activity of various physiological enzymes relevant to disease diagnosis.

The present invention discloses a total bilirubin determining reagent box, which is liquid double reagent formed by reagent 1 and reagent 2. The constituents and the consistence range of the reagent 1 are: disodium of 13.5-81g / L, sodium dihydrogenphosphate of 0.6-3.6g / L, sodium chloride of 9.0g / L, ethylenediaminetetraacetic acid disodium salt of 0.2-1g / L, tween of -80 0.5-2ml / L, and sodium dodecyl sulfate of 1-10g / L. The constituents and the consistence range of the reagent 2 are: disodium of 1g / L, sodium chloride of 9g / L, sodium persulfate of 11.9-23.8g / L, and sulphuric acid of 0.1-2ml / L. The total bilirubin determining reagent box is stable and environment-friendly, and has proper linearity range, high test accuracy, high precision, high sensitivity and strong anti-jamming performance.