The invention discloses a method for producing gamma-
aminobutyric acid by using a banana biological
enzyme method, which comprises the following steps of:
cutting up and
grinding bananas, adding 1-3L of leaching liquor into every 1 kg of bananas, and after the obtained mixture is uniformly stirred, carrying out standing extraction on the obtained mixture for 3-5 h at a temperature of 4-25 DEG C; carrying out
centrifugation on the obtained product for 5-10 min at a speed of 5000-7000 rpm so as to obtain
glutamic acid decarboxylase crude-
enzyme; adding 30-50 g of
glutamic acid and
sodium glutamate with pH of 4.5-6.0 into every 1L of
enzyme, so that the obtained product can be converted completely in 12-24 h under the condition of 37 DEG C; and adding 0.1-3.0% of
active carbon into the obtained product, stirring for 30-60 min, and carrying out filtering, rotary
steaming and
crystallization on the obtained product so as to obtain 20-40 g of gamma-
aminobutyric acid. The method is simple in production process, specific in reaction, low in cost, high in yield, good in purity, short in cycle, less in waste, and environmental-friendly, and leaching liquor can be repeatedly used, therefore, the method is suitable for industrial production.