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Method for preparing cholesterol by microbial conversion method

A technology of microbial transformation and cholesterol, applied in the field of biochemistry, can solve the problems of not conforming to the scientific concept of ecological environmental protection, increasing the production cost of enterprises, consuming a lot of manpower and material resources, etc., and achieving the effects of mild conditions, low pollution and specific reactions.

Active Publication Date: 2014-09-03
HENAN LIWEI BIOLOGICAL PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These methods are chemical methods, use a variety of organic solvents, and easily produce a variety of by-products in the reaction process, which is easy to cause environmental pollution. If it is processed, it will consume a lot of manpower and material resources, which does not conform to the scientific concept of ecological environmental protection and increases cost of production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] A method for preparing cholesterol by microbial transformation, the method is completely transformed by microorganisms, and lanolin is used as raw material to produce cholesterol, and the steps are as follows:

[0026] Soil collection: Soil samples were collected separately at the scouring waste dump and the cholesterol production factory.

[0027] Seed medium: 1000ml of potato juice, 20g of sugar, 20g of agar, 30g of lanolin.

[0028] Fermentation medium: 1000ml potato juice, 90g maltose, 60g agar, 80g lanolin;

[0029] Screen strains

[0030] Take 1.0 g of the soil sample collected above, put it in a sterilized test tube, add 10 ml of sterile water, shake it with a vortex shaker, let it stand, take the supernatant, drop it on the plate medium, and remove it with a glass rod. Spread it evenly, and culture it in a constant temperature incubator at 27°C for 2-5 days. After colonies grow on the plate, pick out a single colony on the isolation medium, judge the pure stra...

Embodiment 2

[0039] A method for preparing cholesterol by microbial transformation, the method is completely transformed by microorganisms, and lanolin is used as raw material to produce cholesterol, and the steps are as follows:

[0040] Soil collection: Soil samples were collected separately at the scouring waste dump and the cholesterol production factory.

[0041] Seed medium: 1000ml of potato juice, 20g of sugar, 20g of agar, 30g of lanolin.

[0042] Fermentation medium: 1000ml potato juice, 90g glucose, 60g agar, 80g lanolin;

[0043] Screen strains

[0044] Take 1.0 g of the soil sample collected above, put it in a sterilized test tube, add 10 ml of sterile water, shake it with a vortex shaker, let it stand, take the supernatant, drop it on the plate medium, and remove it with a glass rod. Spread evenly, and culture in a constant temperature incubator at 27°C for 2-5 days. After colonies grow on the plate, pick out a single colony on the seed medium, judge the pure strain accordin...

Embodiment 3

[0053] A method for preparing cholesterol by microbial transformation, the method is completely transformed by microorganisms, and lanolin is used as raw material to produce cholesterol, and the steps are as follows:

[0054] Soil collection: Soil samples were collected separately at the scouring waste dump and the cholesterol production factory.

[0055] Seed medium: 1000ml of potato juice, 20g of sugar, 20g of agar, 30g of lanolin.

[0056]Fermentation medium: 1000ml potato juice, 90g sucrose, 60g agar, 80g lanolin;

[0057] Screen strains

[0058] Take 1.0 g of the soil sample collected above, put it in a sterilized test tube, add 10 ml of sterile water, shake it with a vortex shaker, let it stand, take the supernatant, drop it on the plate medium, and remove it with a glass rod. Spread it evenly, and culture it in a constant temperature incubator at 27°C for 2-5 days. After colonies grow on the plate, pick out a single colony on the separation medium, judge the pure stra...

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Abstract

The invention discloses a method for preparing cholesterol by a microbial conversion method. The method is characterized in that cholesterol is prepared from raw material lanolin completely by microbial conversion through the steps of: 1) crushing raw material and adding a carbon source and a nitrogen source to prepare a seed medium and a fermentation medium; 2) screening strains; 3) inoculating the screened strain and then stirring and fermenting; and 4) separating, purifying and crystallizing. The method has the characteristics of mild conditions, specific reaction, small pollution and the like in preparing cholesterol. The cholesterol prepared by the method has high purity and yield, and conforms to the concept of environment protection.

Description

technical field [0001] The invention relates to a method for preparing cholesterol by a microbial conversion method, which belongs to the field of biochemistry. Background technique [0002] Lanolin is a multi-component mixture of esters secreted by sheep's sebaceous glands and adhering to the wool duvet. It is mainly an ester formed of fatty acids and roughly equal amounts of fatty alcohols, sterols, and trimethylsterols. Contain about 10% cholesterol in lanolin, cholesterol can be used as a kind of multi-functional cosmetic auxiliary agent with biological activity, and U.S. CTFA (Cosmetic Fragrance and Fragrance Association) Cosmetic Raw Materials Handbook has listed cholesterol as a natural active substance that can be added to cosmetics. Cholesterol is also the main raw material for the synthesis of vitamin D3. There are a lot of lanolin resources in our country, and it is very meaningful to study the technology of extracting cholesterol from them. [0003] At present,...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P33/00
Inventor 马志军薛家禄
Owner HENAN LIWEI BIOLOGICAL PHARMA
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