Total bilirubin determination reagent kit
The technology of total bilirubin and kit is applied in the field of kits for determination of total bilirubin by sodium persulfate oxidation method, which can solve the problems of high sample blank, fluctuation of reaction curve, decrease of pH value of reagents, etc. The effect of strong lipid turbidity, high accuracy and precision, and long storage time
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Embodiment 1
[0067] Components of reagent 1 (R1) and their concentration ranges:
[0068] Disodium hydrogen phosphate 13.5g / L
[0069] Sodium dihydrogen phosphate 0.6g / L
[0071] Disodium edetate 0.2g / L
[0072] Tween-80 0.5ml / L
[0073] Sodium Lauryl Sulfate 1g / L
[0074] The components of reagent 2 and their concentration ranges are as follows:
[0075] Sodium dihydrogen phosphate 1g / L
[0076] Sodium chloride 9g / L
[0077] Sodium persulfate 12g / L
[0078] Sulfuric acid 2ml / L
[0079] The configuration method of Reagent 1 and Reagent 2 is a conventional method, that is, the components of Reagent 1 and Reagent 2 are added to distilled water and then mixed and stirred evenly.
Embodiment 2
[0081] Components of reagent 1 (R1) and their concentration ranges:
[0082] Disodium hydrogen phosphate 81g / L
[0083] Sodium dihydrogen phosphate 3.6g / L
[0084] Sodium chloride 9g / L
[0085] Disodium EDTA 1g / L
[0086] Tween-80 2ml / L
[0087] Sodium Lauryl Sulfate 10g / L
[0088] The components of reagent 2 and their concentration ranges are as follows:
[0089] Sodium dihydrogen phosphate 1g / L
[0090] Sodium chloride 9g / L
[0091] Sodium persulfate 24g / L
[0092] Sulfuric acid 0.1ml / L
[0093] The preparation method of the kit in Example 2 is the same as in Example 1.
Embodiment 3
[0095] Components of reagent 1 (R1) and their concentration ranges:
[0096] Disodium hydrogen phosphate 60g / L
[0097] Sodium dihydrogen phosphate 2.4g / L
[0098] Sodium chloride 9g / L
[0099] Disodium edetate 0.7g / L
[0100] Tween-80 1.5ml / L
[0101] Sodium Lauryl Sulfate 7.5g / L
[0102] Wherein each component of reagent 2 and its concentration range are as follows:
[0103] Sodium dihydrogen phosphate 1g / L
[0104] Sodium chloride 9g / L
[0105] Sodium persulfate 22g / L
[0106] Sulfuric acid 0.5ml / L
[0107] The preparation method of the kit of Example 3 is the same as that of Example 1.
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