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Method for using tissue culture to separate salt-tolerant tomato

A technique for tissue culture and tomato, applied in the field of screening salt-tolerant tomatoes by combining tissue culture with nutrient solution cultivation, can solve problems affecting identification results, infection, etc., and achieve the effect of verifying reliability and avoiding the infection of diseases, especially viruses

Inactive Publication Date: 2008-06-04
INST OF GARDENING ACAD OF AGRI OF SHANGHAI
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  • Abstract
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Problems solved by technology

Moreover, under natural conditions, tomato seedlings are susceptible to some diseases, especially viruses, which affect the identification results.

Method used

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  • Method for using tissue culture to separate salt-tolerant tomato
  • Method for using tissue culture to separate salt-tolerant tomato
  • Method for using tissue culture to separate salt-tolerant tomato

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Embodiment 1

[0024] (1) Disinfect the seeds of the tested variety, inoculate them in sterilized water, place them in a shaking incubator, and carry out constant temperature shaking and accelerating germination at 25 ± 1°C until the radicle length is 4mm to obtain germinated seeds;

[0025] Said test variety seeds are common cultivars of tomato, which are respectively: Dayu Zhongshu No. 4, Baoda 903, Jiangshu No. 14, American Dahong Qinhuang, Tomato Dahong, Zhongshu No. 4, Japan Dafen Queen, Baofen, Fenbao No. 18, Dayu Tianjin Ginkgo, Suhong 2003, Ginkgo Qiangfeng.

[0026] Said seed disinfection method is as follows: the surface disinfection of 75% ethanol with the volume concentration of the seeds of the tested variety is carried out for 20 seconds, and then 0.1% HgCl 2 Disinfect with aqueous solution for 10 minutes, rinse with sterile water for 4 times;

[0027] The shaking frequency of the shaking incubator is 100r·min -1 .

[0028] (2) NaCl stress treatment, the germinated seeds of ...

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Abstract

The invention discloses a method of further screening salt tolerant tomato from seedlings of tissue culture of certain salinity under hydroponics condition by utilizing tissue culturing in combination with nutrient solution culturing to screen the salt tolerant tomato. The method consists of the following steps: (1) sample seeds provided are sterilized and inoculated in sterilized water and germinated; (2) germinated seeds that are acquired from step (1) are respectively inoculated on MS culture medium for NaCl stress treatment; (3) salt damage investigation and calculation of salt stress indexes; (4) seedlings of tissue culture under NaCl concentration of 90mmol.L<-1> are transported into a greenhouse and transplanted into the nutrient solution of the concentration of 90mmol.L<-1> after seedling recovering. Seedlings are harvested 10 days later, and all indexes of the seedlings are recorded. The method of the invention can assess salt resistance of tomatoes of various breeds more accurately, and is of great significance for providing theoretical basis for screening and utilizing of salt resistant tomato seed resource and establishing salt resistance culture.

Description

technical field [0001] The invention relates to a screening method for salt-tolerant tomatoes, in particular to a method for screening salt-tolerant tomatoes by using tissue culture combined with nutrient solution cultivation. Background technique [0002] Tomato is a kind of vegetable that people like very much. It is rich in nutrition. However, the salt content of the soil has a great influence on the growth of tomato. Screening a salt-tolerant tomato is of great significance for the production of high-quality and high-yield tomato. [0003] At present, conventional screening methods, such as screening only in the field or using seed germination stage screening under tissue culture conditions; methods reported in the literature, such as Liu Zhiyuan reported that tomato seedlings were screened with salt treatment in the greenhouse, and Chen Huoying reported that NaCl stress has different effects on different species. The effect of different varieties of tomato seed germinat...

Claims

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Application Information

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IPC IPC(8): A01H4/00A01G1/00A01G7/06A01G31/00A01C1/00
Inventor 朱为民吴雪霞万延慧朱龙英
Owner INST OF GARDENING ACAD OF AGRI OF SHANGHAI
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