Method for verification of color spectrum fingerprint pattern peak purity

A fingerprint and inspection method technology, which is applied to the inspection of the peak purity of the chromatographic fingerprint of traditional Chinese medicine by its own orthogonal projection method, and the inspection field of the peak purity of the chromatographic fingerprint of traditional Chinese medicine, which can solve the problems of failing to make full use of the spectral information of the combined chromatographic instrument and the like. , to achieve the effect of fast processing

Inactive Publication Date: 2008-09-03
WANNAN MEDICAL COLLEGE
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Problems solved by technology

[0003] None of the above methods can make full use of the spectral information of each effluent component recorded by the coupled chromatographic instrument during the chromatographic separation process.

Method used

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  • Method for verification of color spectrum fingerprint pattern peak purity
  • Method for verification of color spectrum fingerprint pattern peak purity
  • Method for verification of color spectrum fingerprint pattern peak purity

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Experimental program
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Embodiment Construction

[0038] Such as figure 1 As shown, step 101 is: adopt the high-performance liquid chromatograph that contains DAD detector to carry out spectrum analysis to substance, utilize MATLAB language to process, obtain the whole information of spectrum, described information comprises the peak area of ​​corresponding peak, peak height, keep time.

[0039] Step 102 is: extract the chromatographic peak to be identified from all the information of the fingerprint.

[0040] Step 103 is: subtracting the noise signal from the chromatographic peak to be extracted and identified to obtain the chromatographic peak to be identified without instrument interference; the noise signal is not greater than 0.05.

[0041] Step 104 is: setting a standard window in the chromatographic peak from which the noise signal is subtracted, and moving the standard window to establish an orthographic projection matrix.

[0042] Step 105 is: project the chromatographic peak to be identified to the orthographic p...

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Abstract

The invention discloses a method for testing purity of chromatographic fingerprint peak, which includes the following steps: processing the chromatographic fingerprint to the Chinese medicine by HPLC with DAD tester, obtaining whole information of the fingerprints; extracting chromatographic peaks to be identified in the chromatogram; deducting noise signals from the chromatographic peaks to be extracted and identified; setting standard windows; moving windows and establishing an orthogonal projection matrix; projecting chromatographic peaks to be identified to the orthogonal projection matrix, obtaining the residual spectrum; calculating the included angle between the chromatographic peak to be identified and the residual spectrum; determining whether the cosine of the included angle is less than the noise signal; if the cosine of the included angle is less than the noise signal, the chromatographic peak to be identified is a pure peak; if the cosine of the included angle is more than the noise signal, the chromatographic peak to be identified is a mixed peak. Compared with the prior art, the purity of the chromatographic peak is directly judged without standard or reference substance, the process is fast and easy, and is programmed.

Description

technical field [0001] The invention belongs to a method for testing the purity of a chromatographic peak, in particular to a method for testing the peak purity of a chromatographic fingerprint of a traditional Chinese medicine, in particular to a method for testing the peak purity of a chromatographic fingerprint of a traditional Chinese medicine by using a self-orthogonal projection method. Background technique [0002] Accurately identifying the purity of chromatographic peaks is the prerequisite for qualitative and quantitative analysis of chromatograms. Since the chemical components of traditional Chinese medicines are mostly secondary metabolites, the chemical structure differences between them are very small, and their physical and chemical properties are similar. Their chromatographic peaks inevitably overlap on the chromatographic fingerprints, and it is even more important to accurately determine the purity of the chromatographic peaks. Identifying the purity of th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/86G06F17/00
CPCG01N30/8686G01N30/8689
Inventor 邹纯才鄢海燕
Owner WANNAN MEDICAL COLLEGE
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