Traditional Chinese medicine extract for suppressing lung cancer A549 cell proliferation and preparation method thereof

A technology of cell proliferation and extract, which is applied in the field of Chinese medicine extract and preparation for anti-proliferation of lung cancer A549 cells, and can solve the problems of toxic and side effects of patients, lack of specificity of radiotherapy and chemotherapy, etc.

Inactive Publication Date: 2011-01-19
NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The treatment of tumors including lung cancer has always been a hot spot in clinical medical research and new drug development. However, due to the lack of specificity of traditional radiotherapy and chemotherapy, it often brings relatively large toxic and side effects to patients while achieving curative effect, while traditional Chinese medicine (including single herbs) Traditional Chinese medicine and traditional Chinese medicine compound) have emerged in this respect, and quickly play an important role, and have broad development prospects

Method used

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  • Traditional Chinese medicine extract for suppressing lung cancer A549 cell proliferation and preparation method thereof
  • Traditional Chinese medicine extract for suppressing lung cancer A549 cell proliferation and preparation method thereof
  • Traditional Chinese medicine extract for suppressing lung cancer A549 cell proliferation and preparation method thereof

Examples

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Comparison scheme
Effect test

Embodiment 1

[0052] Example 1: Anti-proliferative traditional Chinese medicine extract of lung cancer A549 cells and its preparation method

[0053] (1) Prescription:

[0054] 10g of winter melon seeds, 12g of Pinellia chinensis, 10g of Dannanxing, 12g of shiitake mushrooms, 10g of cat's claw, 10g of golden buckwheat, 10g of Houttuynia cordata, 15g of agrimony, 12 parts of sumac, 15g of safflower, 10g of sand ginseng, Polygonatum 10g, Ophiopogon japonicus 10g.

[0055] (2) Preparation method:

[0056] 1. Preparation of prescription extract:

[0057] Take 10g of winter melon seeds, 12g of Pinellia chinensis, 10g of Dannanxing, 12g of mountain twigs, 10g of cat's claw, 10g of golden buckwheat, 10g of Houttuynia cordata, 15g of Agrimony, 12 parts of sumac, 15g of safflower, 10g of sand ginseng , Polygonatum Polygonatum 10g, Ophiopogon japonicus 10g, add 15 times the amount of water to decoct for 0.5 hour, filter and collect the extract, concentrate to get the whole party extract I (0.5g crud...

Embodiment 2

[0062] Example 2: Anti-proliferative traditional Chinese medicine extract of lung cancer A549 cells and its preparation method

[0063] (1) Prescription:

[0064] Winter melon seeds 5g, French pinellia 20g, Dannanxing 5g, mountain twig mushroom 10g, cat's claw 5g, golden buckwheat 5g, houttuynia 5g, agrimony 10g, sumac 20g, safflower 10g, sand ginseng 5g, Polygonatum odoratum 15g, Ophiopogon japonicus 15g.

[0065] 1. Preparation of prescription extract:

[0066] Take 5g of winter melon seeds, 20g of Pinellia chinensis, 5g of Dannanxing, 10g of mountain twigs, 5g of cat's claw, 5g of golden buckwheat, 5g of Houttuynia cordata, 10g of agrimony, 20g of sumac, 10g of safflower, 5g of sand ginseng, jade Bamboo 15g, Ophiopogon japonicus 15g, add 10 times the amount of water to decoct for 1 hour, filter and collect the extract, concentrate to obtain the whole recipe extract I (0.5g crude drug / ml).

[0067] 2. Refinement of prescription extracts:

[0068] Take the whole recipe ex...

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Abstract

Disclosed is a Chinese traditional medicine extractive for resisting lung cancer A549 cells to proliferate, which is composed of the following materials by certain weight ratio: ternate buttercup root, cymose buckwheat rhizome, herba houttuyniae, agrimony, Chinese waxgourd seed, scarlet begonia, brazilwood, safflower, prepared pinellia tuber, arisaema cum bile, root of straight ladybell, polygonatum; the preparing method is that: the above 13 materials are added with water to be decocted and filtered so as to take the filtered liquid out; and then the filtered liquid is condensed to obtain a full extracting liquid 1; and then the extracting liquid 1 is extracted by sherwood oil so as to obtain a prepared extracting liquid 2; and finally, the prepared extracting liquid 2 can be upgraded bymacroporous resin, eluted by pure water, and then eluted by ethanol; the eluted liquid is collected for condensing to 10g medicine / ml so as to obtain the Chinese traditional medicine extractive; indicated by the experiment result of pharmacodynamics, the Chinese traditional medicine extractive has obvious effects on resisting the lung cancer A549 cell proliferation.

Description

technical field [0001] The invention relates to the technical field of traditional Chinese medicines, in particular to a traditional Chinese medicine extract for resisting proliferation of lung cancer A549 cells and a preparation method thereof. Background technique [0002] Lung cancer (lung cancer) originates from bronchial mucosa and alveolar epithelial cells. It is one of the most common malignant tumors in the world today. Due to the rapid development of production, further deterioration of environmental pollution, and the prevalence of bad habits such as smoking, its incidence is on the rise in our country [1，2] . According to the forecast of the World Health Organization (WHO), by 2025, the number of new lung cancer deaths in my country will exceed 1 million each year, and the number of patients will rank first in the world. [3] . [0003] The treatment of tumors including lung cancer has always been a hot spot in clinical medical research and new drug development....

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/898A61P35/00
Inventor 尹莲张旭叶丽红阳美平孙怡周仲瑛
Owner NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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