Fluorescence labeled X-STR locus composite amplification system and use thereof

A compound amplification system and the technology of the compound amplification system, which are applied in the field of genetic markers, can solve the problems of limiting the application of X chromosome genetic markers, poor gene frequency distribution, unfavorable promotion, etc. The result is accurate and the effect of ensuring correctness

Inactive Publication Date: 2009-04-22
SUN YAT SEN UNIV
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AI Technical Summary

Benefits of technology

This patented inventor describes an improved diagnosing tool called Fluorescein Locus Composite Amplified System(FLSAS)that uses specific molecules attached at one end to each part of DNA sequence fragments from two adjacent parts during hybridization process. These markers have unique properties like strong sensory signals, easy handling, long shelf life, stable performance over time, accuracy, reproduction ability, and compatibility with various types of analysis instruments. It allows researchers to identify unknown mutated alleles associated with inherited disorders through their own assay systems without having access to expensive equipment.

Problems solved by technology

The technical problem addressed in this patented text relates to improving the accuracy and efficiency of detecting specific types of microsporons called X-Chromoëtellites (SCY). Existing methods involve analyzing each individual's entire set of X Chrome Loci (Coxs) that contains thousands of repetitive sequences known about 3 million bases long per 100 kilobase bones located within their own body. By identifying certain repeated elements associated with disease trachoms like Down syndrome, Fanconi Anemia, Tayneworth Syndrome, Catheter Abnormalities, and Autism Spectrum Disorder, we aim to develop new molecular markers useful for early child development assessment during sexual fertility tests.

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  • Fluorescence labeled X-STR locus composite amplification system and use thereof
  • Fluorescence labeled X-STR locus composite amplification system and use thereof
  • Fluorescence labeled X-STR locus composite amplification system and use thereof

Examples

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Embodiment 1

[0055] Example 1 Application of MX-STR Fluorescence-labeled Multiplex Amplification System for Identification of Sisters and Identification of Grandmother and Granddaughter

[0056] Principle: The X chromosome is the human sex chromosome, and X-STR is a type of polymorphic genetic marker on the sex chromosome. The X-STR of a male can only be obtained from his mother in the form of haplotype, and can only be passed on to his daughter. Therefore, sisters born to the same father have 1 identical allele at the X-STR locus. Same paternal origin cannot be ruled out if 1 identical allele at all X-STR loci is present in a sample of suspect sisters, but can be ruled out if no identical allele at 3 or more X-STR loci It is from the same father. Similarly, the granddaughter’s X-STR locus has 1 allele that is the same as her grandmother’s, so the suspected grandmother and granddaughter have 1 of the same alleles in all X-STR loci, and their genetic relationship cannot be ruled out. If th...

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Abstract

The invention discloses a fluorescent-labeled X-STR locus multiplex amplification system and application thereof. The system analyzes ten loci, namely DXS7133, DXS6801, DXS981, DXS7424, DXS6789, DXS7132, GATA165B12, DXS6803, DXS101, and GATA31E08 through multiplex amplification, and primers of the ten loci are labeled by four fluorescences, namely FAM, HEX, TAM, and ROX respectively. The invention can prepare a reagent kit; and as an X-STR locus multiplex amplification regent kit with distinct Chinese characteristics, the reagent kit can be used for gene location of identification in disputed paternity, individual recognition, sex appraisal and X-linked inheritable diseases, particularly for prenatal diagnosis and identification in disputed paternity such as sister relationship claim, half sister relationship claim with half blood, generation-skipping relationship claim and so on.

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Claims

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Application Information

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Owner SUN YAT SEN UNIV
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