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Separation method of nucleated cells derived from bone marrow for bone formation

A technology of nucleated cells and bone marrow, applied in the field of separation of bone marrow-derived nucleated cells for bone formation, can solve problems such as difficulty in applying emergency patients

Inactive Publication Date: 2009-05-13
SEWON CELLONTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
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Problems solved by technology

Although this method has many advantages and effects compared with the existing techniques of autologous transplantation, allogeneic transplantation and bone marrow injection, there are still problems. The proliferation of osteoblasts requires a culture period of 3-4 weeks, which is difficult. Applied to emergency patients or patients requiring repeated surgical operations

Method used

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Effect test

Embodiment

[0023] First, a kit for isolating bone marrow-derived nucleated cells for bone formation is prepared, which has the following composition:

[0024] 1) Two solutions for preserving and cleaning the extracted bone marrow;

[0025] 2) Lysis buffer for red blood cells;

[0026] 3) neutralization buffer;

[0027] 4) maintenance buffer for bone formation; and

[0028] 5) Bone graft syringe.

[0029] The method for isolating bone marrow-derived nucleated cells for bone formation of the present invention can be implemented using the above-mentioned kit composition as follows:

[0030] 1) Extract 2-5 milliliters of bone marrow from the patient, and store the extracted bone marrow in the washing solution. When the bone marrow is extracted from the iliac crest, the amount of pure bone marrow is about 2-5 milliliters, and due to the mixing of surrounding blood components, the amount extracted exceeds the stated amount. Thus, extracting bone marrow in an amount of 2-5 ml can effective...

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Abstract

The present invention relates to a method for separating bone marrow-derived nucleated cells for bone formation. The method comprises: the steps of: washing bone marrow; lysing red blood cells in the bone marrow; neutralizing the bone marrow lysate; purifying nucleated cells from the neutralized bone marrow; and mixing the nucleated cells with a maintenance buffer for bone formation. According to the invention, bone marrow-derived nucleated cells, which can be grafted into a site in need of bone formation or bone defect treatment, can be separated in a rapid and convenient manner. As a result, bone formation in emergency patients or patients, who require repeated surgical operations, can be effectively achieved by separating the nucleated cells for bone formation in surgical locations in a convenient and rapid manner and injecting the separated cells into the patients.

Description

technical field [0001] The present invention relates to a method for isolating bone marrow-derived nucleated cells for bone formation, and more particularly, to a method capable of rapidly and conveniently isolating bone marrow-derived nucleated cells capable of being It is transplanted to the site where bone formation is required or where bone defect treatment is required. According to the method of the present invention, it is possible to efficiently form bone in emergency patients or patients undergoing repeated surgical procedures by isolating nucleated cells used to form bone at the surgical site in a convenient and rapid manner and injecting the isolated cells into the patient. bone. Background technique [0002] According to the report of the World Health Organization (WHO), more than 50% of the elderly aged 65 or older are suffering from chronic bone diseases, and the proportion of fractures caused by osteoporosis has increased by two in the past ten years. times m...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/08C12N5/077
CPCC12N5/0654A61K35/28C12N5/0652
Inventor 朴炫信张在德李赛博张晶皓
Owner SEWON CELLONTECH CO LTD
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