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Total flavone extract of Conyza canadensis as well as preparation method and pharmaceutical use thereof

A kind of technology of total flavonoids and extracts, applied in the field of traditional Chinese medicine

Active Publication Date: 2009-07-08
长春天力健康食品有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In terms of pharmacological research, there is no literature report on the pharmacological effects of Xiaofei Peng

Method used

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  • Total flavone extract of Conyza canadensis as well as preparation method and pharmaceutical use thereof
  • Total flavone extract of Conyza canadensis as well as preparation method and pharmaceutical use thereof
  • Total flavone extract of Conyza canadensis as well as preparation method and pharmaceutical use thereof

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0075] (6), IL-2 preparation and activity detection: Induction of IL-2: the method of aseptically making spleen cell suspension was the same as before, washed twice with PRMI-1640 culture medium, and made into 1 × 10 8 / ml cell suspension, add 100 μl per well into a 96-well culture plate, so that the cells in each well are 5×10 6 indivual. The supernatant was collected by centrifugation and stored in a -20°C refrigerator for later use.

[0076] Preparation of activated splenocyte suspension: aseptic preparation of splenocyte suspension in the same way as before, made into 5×10 6 cells / ml cell suspension, add ConA to make the concentration 5μl / ml, set at 37°C, 5% CO 2 After culturing in saturated humidity for 48 hours, the cells were collected, centrifuged twice, resuspended in PRMII-1640 medium, and used as IL-2 activity detection cells.

[0077] Detection of IL-2 activity: Take IL-2 supernatant stored at -20°C and detect mouse splenocytes, add 100 μl of IL-2 supernatant an...

Embodiment 1

[0117] a. Take the dried whole herb of Xiaofei Peng, add 4 times the amount of 40% ethanol to reflux and extract twice, each time for 1 hour and 3 hours respectively;

[0118] b. Combine the extracts, concentrate to dryness, dissolve the obtained dry product in water, filter, add the filtrate to the treated macroporous resin D101 column, and elute through the macroporous adsorption resin D101 column, first elute with water, until washed The eluate is colorless, and then eluted with 10% ethanol, and then the 10% ethanol eluate is concentrated and dried to obtain the total flavonoids extract of P.

Embodiment 2

[0120] a. Take the dried whole herb of Xiaofei Peng, add 6 times the amount of 70% ethanol to reflux and extract for 3 times, each time for 1 hour, 2 hours and 3 hours respectively;

[0121] b. Combine the extracts, concentrate to dryness, dissolve the obtained dry product in water, filter, add the filtrate to the treated macroporous resin DM301 column, and elute through the macroporous adsorption resin DM301 column, first elute with water, until washed The deliquored is colorless, and then eluted with 70% ethanol, and then the 70% ethanol is eluted, concentrated and dried to obtain the total flavonoids extract of P.

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Abstract

The invention relates to a horseweed herb general flavone extract, a preparation method thereof and the medical application, belonging to the field of traditional Chinese medicines. The preparation method comprises the following steps: a. horseweed herb is integrally dried, 40-95% ethanol or methanol with the volume of 4 to 8 times of that of the horseweed herb is added into the horseweed herb, the obtained mixture is processed by reflux extraction for 2 to 4 times, and each time lasts for 1 to 3 hours; b. extracted solutions are combined and are concentrated to be dry, water is added into for dissolving the obtained dry products, the mixture is filtered, and filtered liquid is placed on a processed macroporous resin column; or, the ethanol or the methanol is added into for dissolving the dry products, the macroporous resin is used for proportioning samples, and the proportioned samples are placed on the processed macroporous resin column; and after being eluted by the macroporous absorption resin column, the proportioned samples are eluted by water until the eluted liquid become colorless, the eluted liquid is eluted by 10-90% ethanol with 4 to 7 cylindrical volumes, and finally, the eluted liquid is recycled and is concentrated to be dry, thereby gaining the horseweed herb general flavone extract. The invention has the beneficial effects of filling in the gap of preparing the horseweed herb general flavone extract and expanding the range of application of the horseweed herb general flavone extract in preparing antineoplastic medicines and adjuvant antineoplastic medicines.

Description

technical field [0001] The invention belongs to the field of traditional Chinese medicine. Background technique [0002] The small flying canopy is the whole herb of the genus E. canadensis L. of the family Asteraceae. It is an annual herb that grows on hillsides, grasslands or fields, and roadsides. Distributed in Northeast China and Shanxi, Inner Mongolia, Zhejiang, Fujian, Jiangxi, Shandong, Henan, Hubei, Guangxi, Sichuan, Yunnan, Shaanxi and Taiwan. [0003] Plants of the genus Phaleria contain a variety of chemical components. So far, natural products obtained from plants of this genus include phenylpropanoids, flavonoids, terpenoids, alkynes, and coumarins. The study found that phenylpropanoids and flavonoids are the main characteristic components of the genus Filamentus, and they are also the most concentrated parts of the current research. According to the Chinese Medicine Dictionary, the whole plant of Xiaofei Peng contains volatile oil, and the aerial part contai...

Claims

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Application Information

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IPC IPC(8): A61K36/28A61P35/00
Inventor 严铭铭赵大庆刘威何晓红毕胜男邵帅
Owner 长春天力健康食品有限公司
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