Total flavone extract of Conyza canadensis as well as preparation method and pharmaceutical use thereof
What is Al technical title?
Al technical title is built by PatSnap Al team. It summarizes the technical point description of the patent document.
A kind of technology of total flavonoids and extracts, applied in the field of traditional Chinese medicine
Active Publication Date: 2011-09-07
长春天力健康食品有限公司
View PDF0 Cites 0 Cited by
Summary
Abstract
Description
Claims
Application Information
AI Technical Summary
This helps you quickly interpret patents by identifying the three key elements:
Problems solved by technology
Method used
Benefits of technology
Problems solved by technology
[0004] In terms of pharmacological research, there is no literature report on the pharmacological effects of Xiaofei Peng
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more
Image
Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
Click on the blue label to locate the original text in one second.
Reading with bidirectional positioning of images and text.
Smart Image
Examples
Experimental program
Comparison scheme
Effect test
preparation example Construction
[0073] (6), IL-2 preparation and activity detection: induction of IL-2: aseptic preparation of spleen cell suspension method is the same as before, washed 2 times with PRMI-1640 culture medium, made into 1 × 10 8 / ml cell suspension, add 100 μl per well into a 96-well culture plate, so that the cells in each well are 5×10 6 indivual. The supernatant was collected by centrifugation and stored in a -20°C refrigerator for later use.
[0074] Preparation of activated splenocyte suspension: aseptic preparation of splenocyte suspension in the same way as before, made into 5×10 6 cells / ml cell suspension, add ConA to make the concentration 5μl / ml, set at 37°C, 5% CO 2 After culturing in saturated humidity for 48 hours, the cells were collected, centrifuged twice, and resuspended in PRMI-1640 culture medium, which were used as IL-2 activity detection cells.
[0075] Detection of IL-2 activity: Take IL-2 supernatant stored at -20°C and detect mouse splenocytes, add 100 μl of IL-2 su...
Embodiment 1
[0115] a. Take the dried whole herb of Xiaofei Peng, add 4 times the amount of 40% ethanol to reflux and extract twice, each time for 1 hour and 3 hours respectively;
[0116] b. Combine the extracts, concentrate to dryness, dissolve the obtained dry product in water, filter, add the filtrate to the treated macroporous resin D101 column, and elute through the macroporous adsorption resin D101 column, first elute with water, until washed The eluate is colorless, and then eluted with 10% ethanol, and then the 10% ethanol eluate is concentrated and dried to obtain the total flavonoids extract of P.
Embodiment 2
[0118] a. Take the dried whole herb of Xiaofei Peng, add 6 times the amount of 70% ethanol to reflux and extract for 3 times, each time for 1 hour, 2 hours and 3 hours respectively;
[0119] b. Combine the extracts, concentrate to dryness, dissolve the obtained dry product in water, filter, add the filtrate to the treated macroporous resin DM301 column, and elute through the macroporous adsorption resin DM301 column, first elute with water, until washed The deliquored is colorless, and then eluted with 70% ethanol, and then the 70% ethanol is eluted, concentrated and dried to obtain the total flavonoids extract of P.
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more
PUM
Login to view more
Abstract
The invention relates to a horseweed herb general flavone extract, a preparation method thereof and the medical application, belonging to the field of traditional Chinese medicines. The preparation method comprises the following steps: a. horseweed herb is integrally dried, 40-95% ethanol or methanol with the volume of 4 to 8 times of that of the horseweed herb is added into the horseweed herb, the obtained mixture is processed by reflux extraction for 2 to 4 times, and each time lasts for 1 to 3 hours; b. extracted solutions are combined and are concentrated to be dry, water is added into for dissolving the obtained dry products, the mixture is filtered, and filtered liquid is placed on a processed macroporous resin column; or, the ethanol or the methanol is added into for dissolving thedry products, the macroporous resin is used for proportioning samples, and the proportioned samples are placed on the processed macroporous resin column; and after being eluted by the macroporous absorption resin column, the proportioned samples are eluted by water until the eluted liquid become colorless, the eluted liquid is eluted by 10-90% ethanol with 4 to 7 cylindrical volumes, and finally,the eluted liquid is recycled and is concentrated to be dry, thereby gaining the horseweed herb general flavone extract. The invention has the beneficial effects of filling in the gap of preparing the horseweed herb general flavone extract and expanding the range of application of the horseweed herb general flavone extract in preparing antineoplastic medicines and adjuvant antineoplastic medicines.
Description
technical field [0001] The invention belongs to the field of traditional Chinese medicine. Background technique [0002] The small flying canopy is the whole herb of the genus E. canadensis L. of the family Asteraceae. It is an annual herb that grows on hillsides, grasslands or fields, and roadsides. Distributed in Northeast China and Shanxi, Inner Mongolia, Zhejiang, Fujian, Jiangxi, Shandong, Henan, Hubei, Guangxi, Sichuan, Yunnan, Shaanxi and Taiwan. [0003] Plants of the genus Phaleria contain a variety of chemical components. So far, natural products obtained from plants of this genus include phenylpropanoids, flavonoids, terpenoids, alkynes, and coumarins. The study found that phenylpropanoids and flavonoids are the main characteristic components of the genus Filamentus, and they are also the most concentrated parts of the current research. According to the Chinese Medicine Dictionary, the whole plant of Xiaofei Peng contains volatile oil, and the aerial part contai...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more
Application Information
Patent Timeline
Application Date:The date an application was filed.
Publication Date:The date a patent or application was officially published.
First Publication Date:The earliest publication date of a patent with the same application number.
Issue Date:Publication date of the patent grant document.
PCT Entry Date:The Entry date of PCT National Phase.
Estimated Expiry Date:The statutory expiry date of a patent right according to the Patent Law, and it is the longest term of protection that the patent right can achieve without the termination of the patent right due to other reasons(Term extension factor has been taken into account ).
Invalid Date:Actual expiry date is based on effective date or publication date of legal transaction data of invalid patent.
Login to view more 
Login to view more