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Soybean PLP enzyme, encoding gene and application thereof

A technology for encoding genes and soybeans, applied in application, genetic engineering, plant genetic improvement, etc., can solve the problem of reducing the effectiveness of other amino acids, and achieve the effect of improving quality

Inactive Publication Date: 2010-11-03
NANJING AGRICULTURAL UNIVERSITY
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  • Claims
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Problems solved by technology

However, the content of sulfur-containing cysteine ​​and methionine is low, which is the limiting factor for the nutritional value of soybean protein. The first limiting amino acid in soybean protein is methionine, which limits and reduces the effectiveness of other amino acids.

Method used

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  • Soybean PLP enzyme, encoding gene and application thereof
  • Soybean PLP enzyme, encoding gene and application thereof
  • Soybean PLP enzyme, encoding gene and application thereof

Examples

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Embodiment Construction

[0026] The methods used in the following examples are conventional methods unless otherwise specified.

[0027] 1. cDNA cloning and identification of soybean GmCBL and its coding gene

[0028] Soybean (Glycine max (L.) Merr.) material Nannong 88-31 (abbreviated as NN88-31) (Yu Benshui, Guide to China Planting Technology and Breeding Achievements, 2003, 237) was provided by the Germplasm Resources Research Office of the National Soybean Improvement Center of Nanjing Agricultural University Provided, the material is planted in the net room, and the conventional field management. Based on the cystathionine β-lyase (cystathionine β-lyase) gene reported and identified on the model plant Arabidopsis thaliana (AtHCBL, L40511), and based on the EST information of soybean and the homology analysis of amino acid sequences between species, the sequence was spliced 1. Analysis, which has the nucleotide sequence of SEQ ID NO.1 in the sequence listing and encodes the amino acid residue seq...

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Abstract

The invention discloses soybean PLP enzyme as well as an encoding gene and application thereof, which belong to the field of biotechnology. The soybean PLP enzyme is named GmCBL enzyme and has protein of an amino acid sequence of SEQ ID NO.2 in a sequence table. The encoding gene of the soybean PLP enzyme is a DNA sequence of SEQ ID NO.1 of a GmCBL gene. The soybean PLP enzyme and the encoding enzyme thereof can be used for cultivating high-content methionine plant varieties, particularly high-content methionine soybean varieties. Compared with non-transgenic tobacco, the methionine content of tobacco with over-expression GmCBL remarkably increases, so the GmCBL gene plays an important role in increasing the methionine content of plants.

Description

technical field [0001] The invention relates to the field of plant genetic engineering. Specifically related to pyridoxal-5'-phosphate-dependent carbon-sulfur (C-S) lyase (pyridoxal-5'-phosphate-dependent carbon-sulfur (C-S) lyase, PLP) and its coding gene and application related to methionine synthesis, It also relates to the PLP enzyme GmCBL related to methionine synthesis derived from soybean and its coding gene and its application in cultivating high-content methionine plant varieties. Background technique [0002] Soybean [Glycine max (L.) Merr.] is the main grain and oil crop in the world, and it is the main source of high-quality vegetable protein in human and animal food. Depending on the cultivation conditions and growth environment, soybeans contain 30-55% protein, and the demand for soybean protein exceeds that of the most common consumption food resources. However, the content of sulfur-containing cysteine ​​and methionine is low, which is the limiting factor f...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N9/88C12N15/60C12N15/63C12N1/00C12N1/21C12N15/11C12N15/82
Inventor 喻德跃宁慧霞姚瑶
Owner NANJING AGRICULTURAL UNIVERSITY
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