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SNP markers, intervals, primers and applications related to methionine content in soybean seeds

A seed methionine and methionine technology, applied in recombinant DNA technology, biochemical equipment and methods, and microbial determination/inspection, etc., can solve the problems of undiscovered, genetic improvement molecular marker-assisted selection breeding difficulties, etc., to improve efficiency, reduce Choice cost, reliable use effect

Inactive Publication Date: 2021-03-09
JILIN ACAD OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, no SNP (Single nucleotide polymorphism, single nucleotide polymorphism) molecular markers related to the methionine content traits of soybean seeds have been found, which brings difficulties to the genetic improvement of soybean seed methionine content traits and molecular marker-assisted selection breeding

Method used

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  • SNP markers, intervals, primers and applications related to methionine content in soybean seeds
  • SNP markers, intervals, primers and applications related to methionine content in soybean seeds
  • SNP markers, intervals, primers and applications related to methionine content in soybean seeds

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Embodiment Construction

[0023] The present invention will be described in detail below in conjunction with the accompanying drawings and specific embodiments, but should not be construed as a limitation of the present invention. Unless otherwise specified, the technical means used in the following examples are conventional means well known to those skilled in the art, and the materials, reagents, etc. used in the following examples, unless otherwise specified, can be obtained from commercial sources.

[0024] In the following examples, 3000 copies of soybean germplasm resources were widely collected in the north of 40°N in my country, and the content of methionine in soybean seeds was determined by high performance liquid chromatography. According to the distribution of methionine in the resources, each distribution group was sampled, and then Compare the genetic diversity of the sampling population and the total population, and determine the number of sampling samples when they are consistent. A total...

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Abstract

The invention belongs to the technical field of soybean molecular biology, and in particular relates to a SNP marker, interval, primer and application related to the methionine content of soybean seeds. The SNP marker is located at position 3233657 of soybean chromosome 10, and the nucleotide of the molecular marker is G or A; the nucleotide sequence containing the marker sequence is shown in SEQ ID NO.1; the marker interval It is the 3223657-3243657 interval of soybean chromosome 10. The SNP marker is a co-dominant marker, reliable and easy to use, which provides great convenience for the breeding of soybean lines with high methionine content, and can be applied to molecular marker-assisted selection in the improvement of soybean seed methionine content and in the improvement of seed methionine content. The fine mapping of related genes and the application of map-based cloning can accelerate the improvement process of soybean seed methionine content traits.

Description

technical field [0001] The invention belongs to the technical field of soybean molecular biology, and specifically relates to a SNP marker, interval, primer and application related to the methionine content of soybean seeds. Background technique [0002] Soybean protein is one of the important sources of nutrition for humans and poultry. Its amino acid composition is similar to that of milk protein. It is a relatively complete protein, except for the lack of methionine necessary for human body. The quality of soybean protein affects the market competitiveness of soybean products, and the content of methionine is the primary limiting factor. The methionine content in soybeans is generally only 12mg / g protein, which is far from the 26mg / g recommended by the World Health Organization and the Chinese Nutrition Society, which meets the nutritional and health needs of the human body. In order to meet people's increasing nutritional needs, increasing the methionine content of cult...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 王跃强王洋邱红梅侯云龙马晓萍高淑芹陈健胡金海王丽
Owner JILIN ACAD OF AGRI SCI
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