Two-way device for collecting magnetotactic bacteria

Abstract

The invention provides a two-way device for collecting magnetotactic bacteria, and relates to the technology of bacteria collection. A collector of the device is a container of which the upper end is provided with an opening; side walls of the container are oppositely provided with an opening respectively; a tubular separation tube is arranged in two openings respectively; a sample tube is sleeved on the outer ends of two separation tubes respectively; two sample tubes are communicated with an inner cavity of the collector through the separation tubes respectively; the collector is arranged between two Helmholtz coils; the two Helmholtz coils are opposite, in parallel and vertically arranged; the two separation tubes on the collector face one coil respectively; and the axes of the separation tubes are consistent with the direction of a magnetic force line; and the two coils are electrically connected with a controller respectively. The device applies uniform weak field to a sample containing the magnetotactic bacteria, makes a magnetic field between 0.1 and 1 mT, can simultaneously collect the magnetotactic bacteria moving along or against the magnetic force line and can effectively and rapidly collect the maximum magnetotactic bacteria without preference from an environment. The device has the advantages of simple structure, low cost and convenient use, and provides high-quality equipment for researches of the diversity and magnetic properties of the magnetotactic bacteria.

Description

technical field [0001] The invention relates to the technical field of bacteria collection, and is a novel two-way collection device for separating and purifying magnetotactic bacteria from the environment by means of a uniform magnetic field. Background technique [0002] Magnetotactic bacteria are a class of Gram-negative bacteria that can move along the direction of a magnetic field. They are widely distributed and can be found in lakes, oceans, and even wet soils. The study of the formation and magnetic properties of the magnetosomes in magnetotactic bacteria not only helps to reveal the process of biomineralization and explore the mechanism of bio-induced changes in the geomagnetic field, but also is an important field of paleomagnetism, rock magnetism and environmental magnetism. research content. In addition, magnetite of bacterial origin also shows extraordinary charm in modern biological and medical applications. Therefore, since the 1990s, the study of magnetotac...

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Problems solved by technology

However, these methods have two obvious disadvantages: one is that bacteria that move against the magnetic field lines cannot be collected, and more and more observations and test results show that there are a large number of bacteria that move against the magnetic force lines in the environment; The geomagnetic field in the environment is a low-intensity uniform field (about 0.05 millite), while the traditional method applies a high-intensity gradient field to the sample, and these external magnetic fields will have adverse effects on magnetotactic bacteria. will affect the collection

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