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Environmental microorganism detection method and system

A technology of environmental microorganisms and detection methods, applied in the field of environmental microorganism detection methods and systems, can solve problems such as difficult to identify trace species

Active Publication Date: 2013-05-08
SHENZHEN HUADA GENE INST +1
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0007] The purpose of the present invention is to provide an environmental microorganism detection method and system, aiming to solve the problem that the existing environmental microorganism detection method is difficult to identify trace species

Method used

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  • Environmental microorganism detection method and system
  • Environmental microorganism detection method and system
  • Environmental microorganism detection method and system

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Embodiment Construction

[0032] In order to make the object, technical solution and advantages of the present invention clearer, the present invention will be further described in detail below in conjunction with the accompanying drawings and embodiments. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.

[0033] In the embodiment of the present invention, high-throughput sequencing technology is used to sequence the DNA extracted from environmental samples to obtain a DNA tag sequence. After removing possible carrier contamination in the DNA tag sequence, the DNA tag sequence is combined with the existing The known sequences in the known database are compared to obtain the classification of the DNA tag sequence.

[0034] figure 1 The implementation process of the environmental microorganism detection method provided by the embodiment of the present invention is shown, and the details are as follows...

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Abstract

The invention is applicable to the field of biological engineering, and provides an environmental microorganism detection method and a system thereof. The method comprises the following steps: sequencing the DNA extracted from environment samples by the high throughput sequencing technology to obtain a DNA tag sequence; eliminating carrier pollution existing in DNA tag sequence; comparing the DNAtag sequence with the known sequence in the known database, and determining the category to which the DNA tag sequence belongs according to the comparison result. The embodiment of the invention can detect which microorganism species or which class of microorganism species possibly exists in environment samples.

Description

technical field [0001] The invention belongs to the field of bioengineering, in particular to an environmental microorganism detection method and system. Background technique [0002] Both proteins and RNA molecules that determine biological traits store information in biological cells in the form of coding sequences of the four bases of DNA. This DNA molecule contains the complete set of genetic information of an organism. In order to understand the function and role of genetic information from an overall perspective, the most important step is to determine the complete set of genetic information of the organism, that is, to know the sequence of all DNA bases of the organism. Traditional genome sequencing mainly uses the "Sanger" sequencing technology, also known as the "terminal termination" sequencing technology. The biggest disadvantage of this sequencing method is: high cost and low yield. In recent years, the "next-generation high-throughput sequencing technology" r...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68C12M1/34G06F19/24
Inventor 覃俊杰李瑞强张秀清王俊杨焕明汪建
Owner SHENZHEN HUADA GENE INST
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