Microorganism detection and identification method and system

A detection method and technology of environmental microorganisms, applied in the direction of microbial determination/inspection, biochemical equipment and methods, etc., can solve problems such as difficult to identify trace species

Inactive Publication Date: 2017-04-05
TIANJIN FUDE XINTAI BIOTECH CO LTD
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Problems solved by technology

[0007] The purpose of the present invention is to provide a method and system for detecting and identifying microorganisms, aiming at solving the problem that the existing environmental microorganism detection methods are difficult to identify trace species

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  • Microorganism detection and identification method and system
  • Microorganism detection and identification method and system
  • Microorganism detection and identification method and system

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Embodiment Construction

[0032] In order to make the object, technical solution and advantages of the present invention clearer, the present invention will be further described in detail below in conjunction with the accompanying drawings and embodiments. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.

[0033] In the embodiment of the present invention, high-throughput sequencing technology is used to sequence the DNA extracted from environmental samples to obtain a DNA tag sequence. After removing possible carrier contamination in the DNA tag sequence, the DNA tag sequence is combined with the existing The known sequences in the known database are compared to obtain the classification of the DNA tag sequence.

[0034] figure 1 The implementation process of the environmental microorganism detection method provided by the embodiment of the present invention is shown, and the details are as follows...

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Abstract

The invention is suitable for the field of bioengineering, and provides a microorganism detection and identification method and system. The method comprises the following steps that sequencing is conducted on DNA extracted from an environmental sample through a high-throughput sequencing technology, and a DNA tag sequence is obtained; vector contaminates existing in the DNA tag sequence are removed; the DNA tag sequence is compared with a known sequence in a known database, and the category to which the DNA tag sequence belongs is determined according to a comparison result. According to the microorganism detection and identification method and system, some microorganism species or one kind of microorganism species which possibly exist in the environmental sample can be detected.

Description

technical field [0001] The invention belongs to the field of bioengineering, in particular to a method and system for detecting and identifying microorganisms. Background technique [0002] Both proteins and RNA molecules that determine biological traits store information in biological cells in the form of coding sequences of the four bases of DNA. This DNA molecule contains the complete set of genetic information of an organism. In order to understand the function and role of genetic information from an overall perspective, the most important step is to determine the complete set of genetic information of the organism, that is, to know the sequence of all DNA bases of the organism. Traditional genome sequencing mainly uses the "sanger" sequencing technology, also known as the "end termination" sequencing technology. The biggest disadvantage of this sequencing method is: high cost and low yield. In recent years, the "next-generation high-throughput sequencing technology" ...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12Q1/04
CPCC12Q1/6888C12Q1/6869C12Q2535/122C12Q2539/10
Inventor 李寿乐
Owner TIANJIN FUDE XINTAI BIOTECH CO LTD
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