Method for improving rapid differentiation and bud formation of anthurium andraeanum callus

Abstract

The invention relates to a method for improving rapid differentiation and bud formation of anthurium andraeanum callus, and relates to a culture method of authurium andraeanum seedling, which comprises the following steps that: A. the anthurium andraeanum variety which is difficult to induce the callus or the callus of which is difficult to be differentiated to adventitious buds is selected as seed; B. watering and fertilizer and medicine application are stopped for one week before the seed production, and the leaf surface is maintained clean; C. the leaves which are just unfolded but is not changed are selected as explant materials; D. the callus is induced; E. adventitious buds are differentiated. Different culture medium formulas and different cutting technologies are used at differentkey stages during the induction of the anthurium andraeanum callus and the bud differentiation so as to realize the rapid induction and rapid bud differentiation. By adopting the method, the clone ofthe anthurium andraeanum varieties which are difficult to develop is effectively established, and the development of the factory-oriented production of the test tube plantlet of different anthurium andraeanum varieties can be realized.

Description

technical field [0001] The invention relates to a method for cultivating anthurium tissue culture seedlings, in particular to a method for improving the rapid differentiation of anthurium callus into buds. technical background [0002] Anthurium andraeanum (Anthurium andraeanum) is a perennial epiphytic evergreen herb belonging to the genus Anthurium in the Araceae family. Its flowers and leaves are beautiful and elegant, with bright red color, long flowering period, strong ornamental value and high ornamental value. It is one of the most popular rare flowers at home and abroad in recent years. Anthurium is popular both as a cut flower and as a potted flower. In the global tropical flower trade, the sales volume of anthurium ranks second only to orchids. Domestic anthurium seedlings have been mostly dependent on imports. With the development of anthurium production and the urgent need of the market, there are more and more domestic manufacturers engaged in the production ...

AI Technical Summary

Problems solved by technology

However, there are some technical difficulties in the actual production of leaves as explant materials to induce callus to produce adventitious buds. One is that different varieties have different degrees of difficulty in inducing callus, and some varieties of leaves are easier to induce callus, and some varieties are difficult to induce callus, such as dark crystal Anthurium (A.andraeanum'Clavinervium'), foliage Anthurium (A.andraeanum'Jungle King'), green jadeite (A.andraeanum' Casparo') and other varieties; the second is that after induction of callus, the callus is difficult to differentiate into adventitious buds, such as enthusiasm (A.andraeanum'Tropical'), Tanis (A.andraeanum'Tnessee'), and Bao (A.andraeanum'ZiBao') and other varieties; the third is the way to induce adventitious buds from leaves, and there are many variations in seedlings

Due to the existence of these problems, the process of industrialized production of Anthurium tissue culture seedlings has been greatly restricted