Waveguide coupling metal photonic crystal biosensor and detecting method thereof

A metal photonic crystal, biosensor technology, applied in instruments, measuring devices, scientific instruments, etc., can solve the problems of complex structure, high cost, and complicated system operation of biosensor devices.

Active Publication Date: 2014-03-26
BEIJING UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Biosensors based on SPR technology still have the disadvantages of complex device structure, high preparation technology requirements, cumbersome system operation, long test cycle, and high cost.

Method used

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  • Waveguide coupling metal photonic crystal biosensor and detecting method thereof
  • Waveguide coupling metal photonic crystal biosensor and detecting method thereof
  • Waveguide coupling metal photonic crystal biosensor and detecting method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0097] This embodiment is a front transmissive waveguide coupling one-dimensional metal photonic crystal biosensor and its detection method:

[0098] The substrate 21 used in this embodiment is made of glass with thickness D=1mm, and the waveguide layer 22 is made of indium tin oxide (ITO) thin film glass sheet with thickness d=200nm.

[0099] In this embodiment, a one-dimensional gold photonic crystal with a period of Λ=330nm is prepared on the ITO waveguide layer of a glass substrate by a preparation method of interference lithography combined with a solution method, and a waveguide-coupled one-dimensional gold photonic crystal biosensor is obtained. When using this sensor for detection, the detection method is as follows:

[0100] 1) A receptor (antibody) 4 with a specific recognition function for the ligand (antigen) 3 is immobilized on the surface of the metal photonic crystal 23 in the waveguide-coupled one-dimensional metal photonic crystal biosensor to form the initial...

Embodiment 2

[0106] This embodiment is a back transmission type two-dimensional waveguide coupling metal photonic crystal biosensor and its detection method:

[0107] The substrate used in this embodiment is glass with a thickness of D=1mm, and the waveguide layer is made of ITO glass sheet with a thickness of d=200nm.

[0108] In this example, two-dimensional gold photonic crystals with Λ=350nm period in both directions were prepared on the ITO waveguide layer of the glass substrate by the preparation method of interference lithography combined with the solution method, and a waveguide-coupled two-dimensional gold photonic crystal biosensor was obtained. When using this sensor for detection, the detection method is as follows:

[0109] 1) Immobilize the receptor (antibody) 4 that has a specific recognition function for the ligand (antigen) 3 on the surface of the metal photonic crystal in the two-dimensional waveguide-coupled metal photonic crystal biosensor to form the initial diaphragm ...

Embodiment 3

[0115] This embodiment is a front reflective waveguide coupling one-dimensional metal photonic crystal biosensor and its detection method:

[0116] The substrate used in this embodiment is glass with a thickness of D = 1 mm, and the waveguide layer is an ITO glass sheet with a thickness of d = 210 nm.

[0117] In this embodiment, a one-dimensional gold photonic crystal with a period of Λ=400nm is prepared on an ITO waveguide of a glass substrate by a preparation method of interference lithography combined with a solution method, and a waveguide-coupled one-dimensional gold photonic crystal biosensor is obtained;

[0118] 1) Immobilize the antibody with specific recognition function on the antigen on the surface of the metal photonic crystal in the waveguide-coupled one-dimensional metal photonic crystal biosensor to form the initial diaphragm of the sensor, and contact the blank sample solution with the surface of the metal photonic crystal 23 ;

[0119] 2) The spectral test ...

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Abstract

The invention relates to a waveguide coupling metal photonic crystal biosensor and a detecting method thereof, which are used for the high-sensitivity sensation of biomolecule concentration and bioactive molecule idiosyncrasy. The waveguide coupling metal photonic crystal biosensor comprises a base, a waveguide layer and metal photonic crystals arranged on the waveguide layer. When the waveguide coupling metal photonic crystal biosensor is used for detecting, light transmitted by a light source irradiates the metal photonic crystals at an angle, and an optical detector is used for detecting an extinction spectrum of transmitted light passing through the waveguide layer and the base or detecting an extinction spectrum of transmitted light passing through the metal photonic crystals; after receptors are fixed on the metal photonic crystals, a sample solution flows through the surfaces of the metal photonic crystals, the detector is used for detecting the extinction spectrum of the transmitted light which passes through the waveguide layer and the base at the moment or detecting the extinction spectrum of the transmitted light which passes through the metal photonic crystals, and the two extinction spectrums are calculated by secondary extinction spectrums to quantificationally detect ligand concentration. The waveguide coupling metal photonic crystal biosensor has high sensitivity, low cost, simple preparation, simple use method, and the like.

Description

technical field [0001] The invention is a waveguide-coupled metal photonic crystal biosensor and its detection method, which is based on the physical mechanism of the strong spectroscopic coupling between the particle plasmon resonance in the metal photonic crystal and the waveguide resonance mode, which can be used for biomolecules (proteins) , sugar molecules, DNA, etc.) and the high-sensitivity sensing of the specific reaction of biologically active molecules, which belong to the cross field of optoelectronic technology and biotechnology. Background technique [0002] Biomolecular sensors with high sensitivity and high resolution characteristics play a very important role in scientific research and practical applications such as biology, life science, and medicine. Biosensors based on spectral response characteristics show unique advantages due to their reliable physical principles and sophisticated technical means. [0003] The basic structure of the traditional surface...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/553G01N21/55G01N21/552
Inventor 张新平刘红梅
Owner BEIJING UNIV OF TECH
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