Quantitative measurement method of ligustilide

Abstract

The invention discloses a quantitative measurement method of ligustilide. In the method, butyl phthalide is used as a substitute reference, high-efficiency liquid chromatography is used for measurement, octadecylsilane chemically bonded silica is used as a filler, a flowing phase consists of methanol and water in a volume ratio of (30-70):(70-30) or acetonitrile and water in a volume ratio of (30-60):(70-40), the detection wavelength is 208 to 334 nanometers, the peak areas A and Ai of the ligustilide in a measured sample and the phthalide substitute reference are measured respectively, and based on the concentration Ci of the butyl phthalide, the concentration C of the measured ligustilide component is calculated by using a correction factor f according to formula below, wherein the correction factor f is between 0.08572 and 1.3283. Experiment results show that the result of the ligustilide content measurement performed by the method is consistent with that of the ligustilide contentmeasurement performed by a method using the freshly prepared ligustilide as a reference, and the measurement result of the method is stable.

Description

technical field The invention relates to a method for quantitatively detecting ligustilide components by substituting a reference substance. Background technique Reference substances are the key to drug quality evaluation and supervision and inspection. In actual work, because some reference substances are expensive, the inspection cost is high, and some reference substances cannot be provided due to instability, which affects the determination of the drug content, making it difficult to control the quality of the drug, which affects the quality of the drug. An important factor in the evaluation and supervision inspection. Ligustilide is the main active ingredient of volatile oils in commonly used traditional Chinese medicines such as Angelica and Chuanxiong. Due to its poor stability, pure ligustilide cannot be used as a reference substance for national legal determination of ligustilide for quantitative detection of ligustilide. In order to solve similar problems, peo...

AI Technical Summary

Problems solved by technology

For above-mentioned situation, the present invention provides a kind of quantitative detection method that replaces reference substance to carry out ligustilide through further research and test, to solve because ligustilide pure product stability is poor can not be used as national legal content determination with reference substance Quantitative detection of ligustilide to achieve quantitative detection and monitoring of drugs containing ligustilide to ensure drug quality

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