MMP2 gene in-situ hybridization detection kit and detection method and use thereof

A detection kit and in situ hybridization technology, applied in the field of kits, can solve problems such as heterogeneity, drug resistance of tumor cells, failure of the anti-cancer war, etc., and achieve the effects of high sensitivity, strong specificity, and convenient operation

Inactive Publication Date: 2011-03-30
SUZHOU FUYING GENE TECH
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] In 2005, the United States Institute of Health, Cancer Institute, Center for Disease Control and other units made an annual report, "Considering that human beings have failed in the war against cancer", that is to say, the mortality rate of cancer has not been reduced. Several factors for the failure of the cancer war a...

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • MMP2 gene in-situ hybridization detection kit and detection method and use thereof
  • MMP2 gene in-situ hybridization detection kit and detection method and use thereof
  • MMP2 gene in-situ hybridization detection kit and detection method and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] An in situ hybridization detection kit for MMP2 gene, comprising a hybridization probe, a marker, and a potentiator, wherein the sequence of the hybridization probe is shown in SEQ ID NO.1. Hybridization probes were labeled with digoxigenin. The composition of other liquids and specimens in the kit is as follows:

[0046] Digestive solution 100μl / tube 1 tube / box Colorless transparent liquid

[0047] Protective solution 100μl / tube 1 tube / box Colorless transparent liquid

[0048] Pre-hybridization solution 1300μl / tube 2 tubes / box Colorless transparent liquid

[0049] Sense hybridization solution 10μl / tube 1 tube / box Colorless transparent liquid

[0050] Antisense hybridization solution 10μl / tube 1 tube / box Colorless transparent liquid

[0051] Blocking solution 1000μl / tube 1 tube / box Colorless transparent liquid

[0052] Alkaline phosphatase antibody 1μl / tube 1 tube / box Colorless transparent liquid

[0053] Chromogen A 175μl / tube 1 tube / box Yellow liquid

[0054] C...

Embodiment 2

[0096] A kind of MMP2 gene in situ hybridization detection method and its kit application

[0097] 1. Specimen processing

[0098] 1. Use a 10ml centrifuge tube to fill 4.5ml of lymphocyte separation medium, then slowly add 3ml of anticoagulated blood into the centrifuge tube containing lymphocyte separation medium (blood: lymphocyte separation medium = 1:1.5), and centrifuge at 2000r / min 10min;

[0099] 2. Take the white blood cells in the middle layer into another centrifuge tube, then add about twice the amount of 1× buffer I to this tube, mix well, and centrifuge at 1500g / min for 10min;

[0100] 3. Discard the supernatant. Add about twice the 1× buffer I to the precipitate, mix well, and centrifuge at 1500g / min for 10min;

[0101] 4. Discard the supernatant, and absorb the excess liquid from the mouth of the test tube with paper towels. Then the precipitate was made into a suspension, dropped on a glass slide, and allowed to dry naturally. (Hospitals with conditions ca...

Embodiment 3

[0137] Parallel experiments between the detection of cervical cancer metastases with the MMP2 gene kit and the detection of cervical cancer metastases with the CA125 gene kit.

[0138] In order to scientifically evaluate the specificity, sensitivity and accuracy of the above genes in cervical cancer metastasis. We used the method of parallel experiments to detect the mRNA of the above genes at the same time. The detection technology used nucleic acid in situ hybridization technology to detect the MMP2 gene and CA125 (CA125 (tumor marker), NM -024690) mRNA of the gene (nucleic acid in situ hybridization, immunohistochemical staining, microscopic counting, result reporting, etc. all adopt the same methods, steps and reagents as the in situ hybridization techniques of Example 1 and Example 2). It was found that the expression level of MMP2 gene in patients with cervical cancer metastasis was higher than that of CA125 gene in patients with the same disease. The results showed tha...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to an MMP2 gene in-situ hybridization detection kit, which comprises a hybridization probe and markers, wherein the sequence of the hybridization probe is represented by the sequence SEQ ID No.1. The invention also provides an MMP2 gene in-situ hybridization detection method and the use of the kit in the preparation of medicaments for detecting cancer early transfer and recurrent diseases. The invention has the advantage that the kit provided by the invention has the characteristics of high sensitivity and high specificity. The detection method of the invention is convenient and simple for operation and can be widely used and promoted in district or above hospitals.

Description

【Technical field】 [0001] The present invention relates to a kit, in particular to an in situ hybridization detection kit for MMP2 gene and its detection method and application 【Background technique】 [0002] In 2005, the United States Institute of Health, Cancer Institute, Center for Disease Control and other units made an annual report, "Considering that human beings have failed in the war against cancer", that is to say, the mortality rate of cancer has not been reduced. Several factors for the failure of the cancer war are: 1. Heterogeneity of tumor cells; 2. Drug resistance of tumor cells; 3. Incomplete design of anticancer drugs. At the same time, the report also proposed that existing cancer diagnosis and treatment measures should be re-examined. The inventor found in the research that another two important reasons for the non-declination of the cancer mortality rate are: 1. Real early diagnosis cannot be achieved; 2. The pathological mechanism of metastasis is not cl...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12Q1/68
Inventor 裘建英张云福
Owner SUZHOU FUYING GENE TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap