Inorganic-organic microbial compound fertilizer and preparation method thereof
A technology of microorganisms and compound fertilizers, applied in the direction of organic fertilizers, etc.
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Embodiment 1
[0055] A kind of preparation method of inorganic-organic microbial compound (mixed) fertilizer, it is characterized in that: comprise the following steps:
[0056] (1) Preparation of microbial powder
[0057] ①Preparation of various bacterial strains
[0058] Preparation of Rhodopseudomonas palustris ACCC 10649 bacterial liquid:
[0059] Under sterile conditions, Rhodopseudomonas palustris ACCC 10649 was inoculated into a liquid medium, the liquid medium being: the content of each component in 1.0L distilled water: yeast powder 3.0g, peptone 3.0g , Magnesium sulfate heptahydrate 0.5g, anhydrous calcium chloride 0.3g, pH value adjusted to 6.8-7.0, placed in a conventional light incubator, 28°C-30°C conventional light culture for 6-7 days, the number of bacteria ≥ 5.0 ×10 9 Cfu / ml seed culture solution, then inoculate into 50L sterile water containing 5% (weight percent) brown sugar, the inoculation amount is 2% of the weight of sterile water, when the dissolved oxygen is 5%,...
Embodiment 2
[0088] (1) Preparation of microbial composite powder
[0089] ①Preparation of various bacterial strains
[0090] According to the method for embodiment 1, the bacterial liquid of each strain is obtained;
[0091] ②Preparation of bacteria powder of various strains
[0092] According to the method of Example 1, the various strains of bacterial liquids prepared in step 1. are used to obtain bacterial powder, in which Pichia pastoris ACCC 20319 is fermented T Bacterial count of bacterial powder ≥ 2.0×10 8 cfu / g, the number of other bacterial powders ≥ 2.0×10 9 cfu / g (Pichia pastoris ACCC 20319 T except bacteria powder);
[0093] ③ Mixing of bacteria powder of various strains
[0094] The bacteria powder of each bacterial species is according to the percentage by weight: Rhodopseudomonas palustris bacteria powder 11%, Pichia fermentum bacteria powder 11%, Lactococcus lactis subsp. lactis bacteria powder 11%, Bifidobacterium bacteria powder 11% , Bacillus subtilis subsp. Stei...
Embodiment 3
[0098] (1) Preparation of microbial composite powder
[0099] ①Preparation of various bacterial strains
[0100] According to the method for embodiment 1, the bacterial liquid of each strain is obtained;
[0101] ②Preparation of bacteria powder of various strains
[0102] According to the method of Example 1, the various strains of bacterial liquids prepared in step 1. are used to obtain bacterial powder, in which Pichia pastoris ACCC 20319 is fermented T Bacterial count of bacterial powder ≥ 2.0×10 8cfu / g, the number of other bacterial powders ≥ 2.0×10 9 cfu / g (Pichia pastoris ACCC 20319 T except bacteria powder);
[0103] ③ Mixing of bacteria powder of various strains
[0104] The bacteria powder of each bacterial species is according to weight percentage: 12% of Rhodopseudomonas palustris bacteria powder, 12% of Pichia fermentum bacteria powder, 12% of Lactococcus lactis subsp. , Bacillus subtilis subsp. stigma powder 11%, Bacillus licheniformis powder 11%, Brevibaci...
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