Method for extracting volatile oil from geotrichum candidum through fermentation of burdock by utilizing supercritical extraction method and application

A supercritical extraction method and burdock seed technology are applied in the field of cigarette spice extraction, which can solve the problems of changing the structure and composition of natural components, being unsuitable for direct application of cigarettes, low yield of burdock seed volatile oil, etc. The effect of harmonizing the aroma of tobacco and enhancing the aroma quality of tobacco

Inactive Publication Date: 2012-08-22
HUBEI CHINA TOBACCO IND +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the method of steam distillation has the following defects: 1. Because steam distillation is high-temperature distillation, it is easy to change the structure of some heat-sensitive substances or react with each other, thereby changing the structure and composition of natural components.
2. Steam distillation takes a long time and consumes a lot of energy
3. The steam distillation method has a low yield of volatile oil from burdock fruit, about 0.2%.
As mentioned above, none of these ingredients are suitable for direct application in cigarettes

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1 Get the fungus Geotrichum candidum strain preserved in the laboratory, inoculate it on the slant medium, place it in a constant temperature incubator at 30°C and cultivate it for 24 hours, and then inoculate it in the liquid medium (K 2 HPO 4 1g, MgSO 4 ·7H 2(0.5 g of peptone, 5 g of peptone, 10 g of glucose, and 1000 mL of water), cultivated at a constant temperature of 30° C. for 24 h, centrifuged the seed liquid and discarded the supernatant, diluted the precipitated bacteria with normal saline and oscillated evenly to obtain an activated seed liquid.

[0027] Chinese herbal medicine burdock root is crushed into coarse powder of 100 mesh sieves, mixed with nutrient solution (peptone 1g, glucose 2g, NaCl 0.05g, K 2 HPO 4 0.01g, MgSO 4 ·7H 2 O 0.05g, water 1000mL) were mixed uniformly at a mass ratio of 20:1, and then sterilized at 115°C for 15min.

[0028] Inoculate the treated seed liquid into the aseptic burdock seed powder according to the mass ra...

Embodiment 2

[0031] Example 2 Get the fungus Geotrichum candidum strain preserved in the laboratory, inoculate it on the slant medium, place it in a constant temperature incubator at 28° C. and cultivate it for 36 hours, and then inoculate it in the liquid medium (K 2 HPO 4 1g, MgSO 4 ·7H 2 (0.5 g of peptone, 5 g of peptone, 10 g of glucose, and 1000 mL of water), cultivated at a constant temperature of 28° C. for 36 hours, centrifuged the seed solution and discarded the supernatant, and diluted the precipitated cells with normal saline for uniform shaking to obtain an activated seed solution.

[0032] Chinese herbal medicine burdock root is crushed into coarse powder of 100 mesh sieves, mixed with nutrient solution (peptone 1g, glucose 2g, NaCl 0.05g, K 2 HPO 4 0.01g, MgSO 4 ·7H 2 O 0.05g, water 1000mL) were mixed uniformly at a mass ratio of 30:1, and then sterilized at 115°C for 20min.

[0033] Inoculate the treated seed liquid into the aseptic burdock seed powder according to t...

Embodiment 3

[0036] Example 3 Get the fungus Geotrichum candidum strains preserved in the laboratory, inoculate it on the slant medium, place it in a constant temperature incubator at 25°C and cultivate it for 48h, and then inoculate it in the liquid medium (K 2 HPO 4 1g, MgSO 4 ·7H 2 O 0.5g, peptone 5g, glucose 10g, water 1000mL), incubate at 25°C for 48 hours at a constant temperature, centrifuge the seed liquid to discard the supernatant, dilute the precipitated bacteria with normal saline and shake evenly to obtain activated seed liquid.

[0037] Chinese herbal medicine burdock root is crushed into coarse powder of 100 mesh sieves, mixed with nutrient solution (peptone 1g, glucose 2g, NaCl 0.05g, K 2 HPO 4 0.01g, MgSO 4 ·7H 2 O 0.05g, water 1000mL) were mixed uniformly at a mass ratio of 50:1, and then sterilized at 115°C for 20min.

[0038] Inoculate the treated seed liquid into the aseptic burdock seed powder according to the mass ratio of 1:50, stir, so that the coarse powder...

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PUM

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Abstract

The invention discloses a method for extracting volatile oil from geotrichum candidum through the fermentation of burdock by utilizing a supercritical extraction method and an application, belonging to the technical field of tobacco spice extraction. The method of the invention adopts the geotrichum candidum to ferment and degrade the grease components harmful to the sensory quality of tobacco, carries out the supercritical carbon dioxide extraction to obtain the burdock volatile oil after the fermentation, has the advantages of effective measures and obvious technical effects, and is simple in operation and easy in implementation, and the extracted volatile oil has the advantages of high yield and good quality. The obtained burdock volatile oil contains more fragrant components same or similar to the tobacco, can be coordinated with the initial fragrance of the tobacco when being added to tobacco shreds, and has the effect of enriching and coordinating the tobacco fragrance, promoting the quality of the tobacco fragrance and reducing the simulation. Particularly, the micro-molecular organic acid generated from the grease components after degradation can effectively enhance the tobacco fragrance and reduces the simulation.

Description

technical field [0001] The invention belongs to the technical field of extracting flavors of cigarettes, and specifically relates to a method for extracting volatile oil from herbal medicine Arctium candidum by fermenting Geotrichum candidum, and using the extracted volatile oil as an additive for cigarettes. Background technique [0002] In the prior art, Burdock Fructus is recorded in "Pharmacopoeia of the People's Republic of China (Part One)" (2005 Edition), which is the dry and mature fruit of Arctium lappa L., a composite plant. Harvest the infructome when the fruit is ripe in autumn, dry it in the sun, and lay down the fruit. It is distributed all over the country, and it was first recorded in "Bielu of Famous Doctors" and listed as a medium grade. Burdock seed is also known as: evil real, mouse sticky, millet sticky, vigorous, bat thorn, Mao Ranran, black wind, hair awl, sticky cubeb, rat tip, curved hook, ten thousand hooks, big cow , Niuzi; its Mongolian medicine...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C11B1/10C11B9/02A24B15/30A24B3/12C12P7/40C12R1/645
CPCY02P20/54
Inventor 黄龙肜霖吴昭
Owner HUBEI CHINA TOBACCO IND
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