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Method for extracting additive from geotrichum candidum fermented burdock by using alcohol and application of additive

An ethanol extraction and tobacco additive technology, which is applied in the field of cigarette flavor extraction, can solve the problems of residue, miscellaneous gas, influence on the application effect of the extract, etc., and achieve the effect of improving the aroma quality and enriching the coordination of tobacco aroma.

Inactive Publication Date: 2011-04-06
HUBEI CHINA TOBACCO IND +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The addition of these oils and fats to cigarettes will cause serious miscellaneous gas, irritation and oral residues, which will affect the quality of cigarettes
If ethanol is used to extract directly, some of these oil components will be extracted at the same time, thus affecting the application effect of the extract in cigarettes

Method used

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  • Method for extracting additive from geotrichum candidum fermented burdock by using alcohol and application of additive
  • Method for extracting additive from geotrichum candidum fermented burdock by using alcohol and application of additive
  • Method for extracting additive from geotrichum candidum fermented burdock by using alcohol and application of additive

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1 Get the fungus Geotrichum candidum strain preserved in the laboratory, inoculate it on the slant medium, place it in a constant temperature incubator at 30°C and cultivate it for 24 hours, and then inoculate it in the liquid medium (K 2 HPO 4 1g, MgSO 4 ·7H 2 (0.5 g of peptone, 5 g of peptone, 10 g of glucose, and 1000 mL of water), cultivated at a constant temperature of 30° C. for 24 h, centrifuged the seed liquid and discarded the supernatant, diluted the precipitated bacteria with normal saline and oscillated evenly to obtain an activated seed liquid.

[0028] Chinese herbal medicine burdock root is crushed into coarse powder of 100 mesh sieves, mixed with nutrient solution (peptone 1g, glucose 2g, NaCl 0.05g, K 2 HPO 4 0.01g, MgSO 4 ·7H 2 O 0.05g, water 1000mL) were mixed uniformly at a mass ratio of 20:1, and then sterilized at 115°C for 15min.

[0029]Inoculate the treated seed liquid into the aseptic burdock seed powder according to the mass ra...

Embodiment 2

[0031] Example 2 Get the fungus Geotrichum candidum strain preserved in the laboratory, inoculate it on the slant medium, place it in a constant temperature incubator at 28° C. and cultivate it for 36 hours, and then inoculate it in the liquid medium (K 2 HPO 4 1g, MgSO 4 ·7H 2 (0.5 g of peptone, 5 g of peptone, 10 g of glucose, and 1000 mL of water), cultivated at a constant temperature of 28° C. for 36 hours, centrifuged the seed solution and discarded the supernatant, and diluted the precipitated cells with normal saline for uniform shaking to obtain an activated seed solution.

[0032] Chinese herbal medicine burdock root is crushed into coarse powder of 100 mesh sieves, mixed with nutrient solution (peptone 1g, glucose 2g, NaCl 0.05g, K 2 HPO 4 0.01g, MgSO 4 ·7H 2 O 0.05g, water 1000mL) were mixed uniformly at a mass ratio of 30:1, and then sterilized at 115°C for 20min.

[0033] Inoculate the treated seed liquid into the aseptic burdock seed powder according to t...

Embodiment 3

[0035] Example 3 Get the fungus Geotrichum candidum strains preserved in the laboratory, inoculate it on the slant medium, place it in a constant temperature incubator at 25°C and cultivate it for 48h, and then inoculate it in the liquid medium (K 2 HPO 4 1g, MgSO 4 ·7H 2 O 0.5g, peptone 5g, glucose 10g, water 1000mL), incubate at 25°C for 48 hours at a constant temperature, centrifuge the seed liquid to discard the supernatant, dilute the precipitated bacteria with normal saline and shake evenly to obtain activated seed liquid.

[0036] Chinese herbal medicine burdock root is crushed into coarse powder of 100 mesh sieves, mixed with nutrient solution (peptone 1g, glucose 2g, NaCl 0.05g, K 2 HPO 4 0.01g, MgSO 4 ·7H 2 O 0.05g, water 1000mL) were mixed uniformly at a mass ratio of 50:1, and then sterilized at 115°C for 20min.

[0037] Inoculate the treated seed liquid into the aseptic burdock seed powder according to the mass ratio of 1:50, stir, so that the coarse powder...

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Abstract

The invention discloses a method for extracting an additive from geotrichum candidum fermented burdock by using alcohol and application of the additive, belonging to the technical field of cigarette spice extraction. In the method, geotrichum candidum with the capacity of efficiently degrading grease is adopted to ferment the burdock, and then the alcohol is utilized to extract the burdock to obtain an alcohol extract. The invention also provides the application of the alcohol extract from the burdock extracted by using the alcohol to the cigarette additive. The method for extracting can effectively degrade the grease component which is not beneficial to cigarette sense organs and generate micromolecular acid components which are beneficial to the cigarette sense organs; the obtained additive contains a large number of fragrant substances required by tobacco and a few substances influencing the tobacco smoking taste and quality. The alcohol extract obtained by the extracting method is applied to the tobacco to take the effect of improving the tobacco product quality.

Description

technical field [0001] The invention belongs to the technical field of cigarette spice extraction, and specifically relates to a method for obtaining ethanol extract by soaking burdock fruit of the botanical medicine through Geotrichum candidum through room temperature immersion, or reflux extraction, or ultrasonic extraction, and the obtained ethanol extraction The application of substances as tobacco additives. Background technique [0002] In the prior art, Burdock Fructus is recorded in "Pharmacopoeia of the People's Republic of China (Part One)" (2005 Edition), which is the dry and mature fruit of Arctium lappa L., a composite plant. Harvest the infructome when the fruit is ripe in autumn, dry it in the sun, and lay down the fruit. It is distributed all over the country, and it was first recorded in "Bielu of Famous Doctors" and listed as a medium grade. Burdock seed is also known as: evil real, mouse sticky, millet sticky, vigorous, bat thorn, Mao Ranran, black wind,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C11B9/00A24B15/30A24B3/12
Inventor 黄龙肜霖吴昭
Owner HUBEI CHINA TOBACCO IND
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