Various important aquagenic zoonoses protozoa simultaneous assay kit and preparation method thereof

Abstract

The invention discloses a various important aquagenic zoonoses protozoa simultaneous assay kit, and further provides a preparation method of the kit. A primer and a probe are designed according to the special gene sequence of the giardia lamblia, the cryptosporidium parvum and the toxoplasma gondii by a multi-fluorescence quantitative PCR (polymerase chain reaction) specific assay method, the sensitivity is improved by optimizing the fluorescence quantitative PCR reaction system and condition, the amplification can be performed, and an amplification result can be directly and timely observed.The invention can be used for fast and exactly assaying three important aquagenic zoonoses protozoa, and has the characteristics of being precise in design, simple and easy to operate, high in sensitivity, high in specificity, exact and objective in judgment, and the like.

Description

technical field [0001] The invention discloses a simultaneous detection kit for multiple important water-borne zoonotic protozoa, which is used for rapid fluorescent quantitative PCR detection of Giardia lamblia, Cryptosporidium parvum and Toxoplasma gondii. The invention also provides the kit The preparation method of the kit belongs to the technical field of parasite detection. Background technique [0002] Giardiasis is an intestinal disease caused by Giardia, with diarrhea as the main symptom. One of the parasitic diseases. [0003] Cryptosporidiosis is a serious zoonosis caused by Cryptosporidium, which can cause severe diarrhea in mammals, especially ruminants and humans. At present, there are 27 species of Cryptosporidium that have been named, and there are many kinds of Cryptosporidium that can infect humans. Among them, the infection of Cryptosporidium parvum is the most common and serious. Cryptosporidium parvum has a wide range of hosts and serious harm. and ot...

AI Technical Summary

Problems solved by technology

[0005] The pathogens of the above three diseases are Giardia lamblia, Cryptosporidium parvum and Toxoplasma gondii, all of which are intracellular parasitic protozoa. Polluted water survives for a long time and is difficult to be killed by ordinary disinfectants, so it brings great difficulties to disease diagnosis, pathogen detection and environmental monitoring. It is urgent to develop a multiple rapid detection kit

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