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Clip based sampling of seed for the removal of specific seed tissue or structures for seed analysis

A seed and sample technology, applied in the direction of analyzing materials, seed and rhizome treatment, sampling devices, etc., to achieve the effect of reducing the amount of site space

Inactive Publication Date: 2011-08-17
PIONEER HI BRED INT INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, automation introduces not only cost and complexity but also real outcomes

Method used

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  • Clip based sampling of seed for the removal of specific seed tissue or structures for seed analysis
  • Clip based sampling of seed for the removal of specific seed tissue or structures for seed analysis
  • Clip based sampling of seed for the removal of specific seed tissue or structures for seed analysis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 2

[0123] 2. Example 2-Cut samples individually with a cigar-type multi-blade cutter ( Figure 2A )

[0124] a) Device ( Figure 2A )

[0125] Figure 2A A single seed sample tool 300 is shown hand or bench mounted. The funnel-shaped housing or body 302 clamps the curved cutting blade 310 of a traditional commercially available cigar cutter 309 (for example, three tangentially moving blades with scissors-shaped handles), which will be referred to as the cutting plane. Some cigar cutters have more than three blades.

[0126] This cigar cutter is available in the market. Examples are the XiMTX multitool available from Xikor, Kansas City, Missouri 64102, and the 8173B Scissor Cigar Cutter available from Cubanoz.biz, 33119 Miami Beach, Florida.

[0127] The hinge cover 306 attached around the body 302 has a rubber or flexible gasket 308 at its center, and the size of the gasket 308 is suitable to accommodate a single corn seed 3 when the seed 3 is pushed into the center of the gasket 308. ...

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Abstract

Apparatus, methods, and systems for useful sampling of seed, wherein viability is maintained, are disclosed. Seed from one generation in a plant advancement experiment is individually sampled by removal and collection of tissue from the seed using a hand-held and manually operated tool having one or more cutting edges. The tissue is then processed to derive one or more biochemical, genetic, or phenotypic characteristic of the seed before a decision is made whether to utilize that seed further in a plant advancement experiment or other plant research and development. In some embodiments of the method, the sampling is controlled to remove a useful amount of tissue for analytical purposes without significant effect on viability potential of the sampled seed. In some embodiments, the sampling is controlled to deter contamination of the sample. In some embodiments, the seed is held in pre-determined orientation to facilitate efficient and accurate sampling.

Description

[0001] This application is a divisional application with the original application number 200780049369.X, the filing date being November 13, 2007, and the title of the invention being "pruning-based seed sampling for seed analysis to remove specific seed tissue or structure". 【Technical Field】 [0002] The invention relates to obtaining tissue samples from a single seed in an efficient way. 【Background technique】 [0003] The common practice in plant breeding or plant evolution experiments is to grow plants from seeds of known sources. The seeds are planted in experimental fields, growth chambers, greenhouses or other growing environments, where they are either cross-pollinated with plants of other known sources or self-pollinated. The seeds produced are progeny from two parent plants or self-pollinated plants, and the seeds are harvested, processed, and planted to continue the plant breeding cycle. To assist in cultivation or to advance the selection process, specific laboratory ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/04G01N1/28A01C1/00
CPCG01N1/28A01C1/025B26B17/00G01N35/00A24F13/24G01N35/0098G01N2033/0077A01G3/00G01N1/04G01N2035/00564B26B13/00G01N33/0077
Inventor S·M·贝克J·科普
Owner PIONEER HI BRED INT INC
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